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[(po)20.8(rt)15(ed)-176.2(th)15(at)-176.3(CM)-182.7(pr)20.9(om)19.6(ot)15(ed)-181.9(bo)20.8(ne)-181.9(gr)20.9(ow)20(th)-180.9([3)20.9(4,)18.5(35)20.8(])-191.1(a)0(n)19.7(d)-191.2(pe)19.7(ri)20.7(od)20.8(on)15.1(ta)19.6(l)]TJ
0 -1.1116 TD
[(ti)20.6(ss)15.5(ue)-521.7(re)19.7(ge)19.7(ne)13.9(ra)19.7(ti)20.6(on)-520.6([3)15.1(6,)24.3(37)15.1(])-531(a)0(n)19.7(d)-531(im)19.5(pr)15.1(ov)20.8(ed)-521.7(bi)15(sp)21(ho)20.8(sp)21(ho)20.8(na)13.9(te)19.5(-)]TJ
T*
[(re)19.7(la)19.6(te)13.8(d)-260.3(o)0(s)21(t)0(e)19.5(o)0(n)20.8(e)0(c)18.5(r)0(o)20.9(s)0(i)15.1(s)-260.1(of)-249.8(th)20.7(e)-261.4(j)0(a)19.6(w)-261.1(in)-250(a)-261.4(r)0(a)19.7(t)-260.4(mo)19.6(de)19.7(l)-260.4([)0(3)15.1(8)0(])20.9(.)]TJ
1.0137 -1.1174 TD
[(Stem)-778.6(cells)-773.9(from)-777.4(human)-779.7(exfoliated)-775.1(decid)-8(uous)-776(teeth)]TJ
-1.0137 -1.1116 TD
[(\(SHEDs\))-522.8(harbor)-520.3(a)-520.6(self-renewal)-525.6(capability)-521.9([39])-522.7(and)-517.1(exist)]TJ
T*
[(within)-238.3(a)-238.4(perivascular)-242.5(niche)-238.4(in)-232.7(the)-240.7(dental)-239.6(pulp.)-238.4(SHEDs)-238.5(have)-237.1(a)]TJ
0 -1.1174 TD
[(high)-402(capacity)-405.4(for)-394.9(proliferation)-408.7(and)-396.1(can)-403(differentiate)-405.3(into)-402.1(a)]TJ
0 -1.1116 TD
[(variety)-338.6(of)-336.2(cell)-341.1(types,)-332.6(including)-339.8(neural)-337.3(cells,)-339.6(adipocytes,)-340.6(os-)]TJ
T*
[(teoblasts,)-326.7(and)-327(chondrocytes)-327.6([40,41].)-324.4(SHEDs)-330.6(secrete)-329(trophic)]TJ
T*
[(factors)-499.5(that)-495.4(promote)-494(neuronal)-500.8(migration,)-494.2(induce)-498.6(vasculo-)]TJ
0 -1.1174 TD
[(genesis)-424.5([32],)-422.5(promote)-424.9(axon)-426(regeneration)-427([40],)-422.5(and)-424.9(protect)]TJ
0 -1.1116 TD
[(neurons)-630.7(from)-627.6(apoptosis)-627.2([33].)-629.8(Therefore,)-628.5(these)-629.8(previous)]TJ
T*
[(studies)-481(support)-479.9(the)-476.9(use)-482.3(of)-474.5(SHEDs)-480.4(as)-481.2(a)-480.3(potential)-479.3(cellular)]TJ
0 -1.1174 TD
[(resource)-340.3(for)-331.5(peripheral)-337.2(nerve)-336.1(regeneration)-340.6(therapies.)]TJ
1.0137 -1.1116 TD
[(The)-449.2(present)-447.7(study)-443.2(focuses)-453.1(on)-445.7(CM)-447.6(containing)-445.7(paracrine)]TJ
-1.0137 -1.1116 TD
[(factors)-390(secreted)-387.7(by)-382.3(cultured)-385.7(SHEDs.)-385.9(Herein,)-387.7(we)-384.4(examined)]TJ
0 -1.1174 TD
[(the)-292.6(inuences)-295.4(of)-290.2(SHED-CM)-300.4(on)-290.2(dorsal)-289.9(root)-292.5(ganglion)-292.5(\(DRG\))]TJ
0 -1.1116 TD
[(neurons)-463.7(and)-465.2(SCs)-464.8(in)-457.3(vitro.)-464.2(Moreover,)-464.7(we)-465(investigated)-466.3(the)]TJ
T*
[(ability)-268.6(of)-261.4(SHED-CM)-271.6(to)-261.5(stimulate)-267.2(nerve)-267(regeneration)-271.5(in)-261.5(vivo)]TJ
T*
[(in)-336.4(a)-330.6(rat)-332.9(sciatic)-339.7(nerve)-336.1(defect)-338.5(model.)]TJ
/F4 1 Tf
9.9626 0 0 9.9626 62.022 297.7511 Tm
[(Mater)-8.1(ials)-337.1(and)-336.9(Methods)]TJ
/F7 1 Tf
0 -1.673 TD
[(Ethic)-8(s)-330.8(stat)-7.8(ement)]TJ
/F3 1 Tf
9.843 0 0 9.843 72 263.2251 Tm
[(Exfoliated)-441.1(deciduous)-444.2(teeth)-445.8(were)-439.5(collected)-446.9(under)-444.4(guide-)]TJ
-1.0137 -1.1116 TD
[(lines)-323.5(approved)-322.1(by)-324.7(Nagoya)-320.8(University)-324.2(\(H-73,)-326.5(2003\).)-319.9(Ethical)]TJ
0 -1.1174 TD
[(approval)-414.4(was)-408.3(obtained)-414.6(from)-414.5(the)-407.8(Ethics)-412.2(Committee)-413.5(of)-411.1(Na-)]TJ
0 -1.1116 TD
[(goya)-333.9(University)-335.8(\(permission)-336.9(number)-337.3(82\).)]TJ
1.0137 -1.1116 TD
[(All)-684.1(experiment)-10.2(al)-683.1(procedures)-693.9(involving)-690(animals)-685.2(were)]TJ
-1.0137 -1.1174 TD
[(conducted)-508.9(according)-514.6(to)-503.4(the)-511.4(National)-507.7(Institutes)-513.4(of)-509(Health)]TJ
0 -1.1116 TD
[(Guide)-249.6(for)-245.1(the)-252.3(Care)-249.6(and)-246.4(Use)-247(of)-249.8(Laboratory)-250.8(Animals)-247.2(and)-246.4(were)]TJ
T*
[(approved)-512.2(by)-514.8(the)-511.4(Nagoya)-516.7(University)-514.3(School)-514.6(of)-509(Medicine)]TJ
T*
[(Animal)-338.4(Care)-336(and)-332.8(Use)-333.4(Committee.)]TJ
/F7 1 Tf
9.9626 0 0 9.9626 62.022 151.1433 Tm
[(Isola)-8.2(tion)-337.5(of)-333.6(SHED)-9(s)-330.8(and)-341.4(cell)-333(cu)-6.4(lture)]TJ
/F3 1 Tf
9.843 0 0 9.843 72 133.285 Tm
[(Human)-210.4(SHEDs)-203.9(were)-209.2(isolated)-212.9(as)-204.8(described)-214.9(previously)-208([41].)]TJ
-1.0137 -1.1116 TD
[(In)-422.6(brief,)-424.9(human)-422.6(exfoliated)-429.6(deciduous)-421.1(teeth)-428.5(were)-422.3(collected)]TJ
T*
[(from)-270.5(patients)-270.5(aged)-271.7(612)-263.6(years.)-276(All)-269.4(patients)-270.5(provided)-269.3(written)]TJ
0 -1.1174 TD
[(informed)-374.1(consent.)-376.3(The)-374.3(dental)-372.1(pulp)-373.2(was)-373.8(separated)-377.3(from)-374.2(the)]TJ
0 -1.1116 TD
[(crown)-237.9(and)-234.9(root.)-238.3(The)-236(isolated)-241.7(pulp)-234.9(was)-235.5(subsequently)-240(digested)]TJ
T*
[(in)-169.4(a)-175(solution)-170.3(of)-175(3)-168.1(mg/mL)-170.6(of)-169.2(collagenase)-179.3(type)-172.7(I)-173.9(and)-171.5(4)-168.1(mg/mL)-170.6(of)]TJ
T*
[(dispase)-205.6(for)-199.1(1)-168.1(h)-196.9(at)-205.1(37)]TJ
/F6 1 Tf
7.8102 0 TD
()Tj
/F3 1 Tf
.3974 0 TD
[(C.)-201.4(Single-cell)-206(suspensions)-202.7(were)-203.4(cultured)]TJ
-8.2076 -1.1174 TD
[(in)-445.8(Dulbeccos)-447.3(modied)-447.8(Eagles)-448.9(medium)-442.3(\(DMEM;)-448.3(Gibco,)]TJ
25.504 67.9242 TD
[(Rockv)-7.7(ille,)-291.6(MD\))-293(supplemented)-294.5(with)-293.5(10%)-292.4(fetal)-290.3(bovine)-295.9(serum)]TJ
0 -1.1174 TD
[(\(FBS\))-536(and)-534.4(antibioticantimycotic)-539.3(solution)-538.9(\(100)-534.3(units/mL)]TJ
0 -1.1116 TD
[(penic)-8(illin)-328.6(G,)-327.9(100)]TJ
/F8 1 Tf
7.1017 0 TD
(m)Tj
/F3 1 Tf
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[(g/mL)-329.5(streptomycin,)-331.4(and)-332.8(0.25)]TJ
/F8 1 Tf
12.0205 0 TD
(m)Tj
/F3 1 Tf
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[(g/mL)-335.3(am-)]TJ
-20.2223 -1.1116 TD
[(photericin)-226.8(B;)-222.2(Gibco\))-221.9(and)-223.3(incubated)-226.8(at)-222.4(37)]TJ
/F6 1 Tf
16.2136 0 TD
()Tj
/F3 1 Tf
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[(C)-221(i)0(n)-221.2(a)0(n)-222.2(atmosphere)]TJ
-16.611 -1.1116 TD
[(of)-272.9(5%)-268.2(CO)]TJ
7.3823 0 0 6.5614 353.3668 679.6912 Tm
(2)Tj
9.843 0 0 9.843 357.0518 681.3352 Tm
[(/95%)-270.5(air.)]TJ
-3.4558 -1.1174 TD
[(Th)19.6(e)-226.9(p)0(r)20.9(i)0(m)19.5(a)0(r)14(y)-225.7(ch)19.7(ar)19.7(ac)18.5(te)19.6(ri)20.7(st)15.1(ic)19.5(s)-225.5(o)0(f)-215.3(t)0(h)20.7(e)-226.9(SH)20.1(ED)18.8(s)-225.5(w)0(e)18.8(r)0(e)-216.4(a)0(s)14.1(s)0(e)19.9(s)0(s)21.2(e)0(d)-216.5(b)0(y)]TJ
-1.0137 -1.1116 TD
[(o)15.2(w)-272.7(c)0(y)19.7(t)0(o)20.7(m)0(e)18.4(t)0(r)15(y)-271.8(as)-262.4(de)19.7(sc)14.1(ri)20.7(be)19.7(d)-271.8(p)0(r)15.1(e)0(v)19.7(i)0(o)20.7(u)0(s)21(l)0(y)-267.3([)0(4)20.9(0)0(])20.9(.)-274.1(Th)19.6(e)-273(S)0(H)14.4(E)15(Ds)-267.8(ex)19.7(-)]TJ
T*
[(hi)14.9(bi)20.7(te)19.6(d)-289.1()0(b)15.2(r)0(o)20.9(b)0(l)20.7(a)0(s)19.9(t)0(i)14.8(c)-290.2(mo)19.6(rp)20.9(ho)20.8(lo)15(gy)-278.7(wi)19.8(th)-284.6(a)-290.2(b)0(i)20.7(p)0(o)20.8(l)0(a)13.8(r)-289(sp)21(in)15(dl)20.7(e)-290.2(s)0(h)21(a)0(p)13.9(e)0(.)]TJ
0 -1.1174 TD
[(Fl)15.1(ow)-279.5(cy)19.7(to)20.7(me)18.4(tr)20.7(y)-289.1(r)0(e)19.7(v)0(e)19.7(a)0(l)19.5(e)0(d)-279.8(t)0(h)20.7(a)0(t)-280(t)0(h)20.7(e)-290.2(SH)20.1(ED)18.8(s)-288.9(e)0(x)19.7(p)0(r)15.1(e)0(s)19.9(s)0(e)19.9(d)-289.1(th)20.7(e)-290.2(m)0(e)18.4(s)0(-)]TJ
0 -1.1116 TD
[(en)13.9(ch)19.7(ym)19.6(al)-159(st)20.9(em)-160.1(ce)18.5(ll)-163.7(ma)18.4(rk)20.9(er)19.7(s,)-159.8(CD)18.9(90)20.8(,)-170.5(C)0(D)18.9(7)0(3)20.8(,)-170.5(an)19.7(d)-173.9(C)0(D)18.9(1)0(0)20.8(5)0(,)-160(b)0(u)20.8(t)-168.3(no)20.8(t)]TJ
T*
[(th)15(e)-232.7(e)0(n)19.7(d)0(o)20.8(t)0(h)20.7(e)0(l)19.5(i)0(a)19.6(l)0(/)14.8(h)0(e)19.7(m)0(a)18.4(t)0(o)20.7(p)0(o)20.8(i)0(e)19.6(t)0(i)20.6(c)-232.6(ma)18.4(rk)20.9(er)13.9(s,)-217.4(CD)18.9(34)15.1(,)-228.1(C)0(D)18.9(4)0(5)20.8(,)-233.8(CD)18.9(11)20.8(b/)]TJ
0 -1.1174 TD
[(c,)-328.2(an)19.7(d)-335.2(h)0(u)20.8(m)0(a)18.4(n)-335.2(le)19.6(uk)20.8(oc)19.7(yt)20.7(e)-336.3(a)0(n)19.7(t)0(i)20.6(g)0(e)19.7(n)-335.2(HL)18.8(A-)20(DR)13.2(.)-331.7(T)0(h)19.6(e)-336.3(SH)20.1(ED)18.8(s)-335(e)0(f)19.7()0(-)]TJ
0 -1.1116 TD
[(ci)13.8(en)19.7(tl)20.6(y)-467.6(d)0(i)14.9(f)0(f)20.9(e)0(r)19.7(e)0(n)19.7(t)0(i)20.6(a)0(t)13.8(e)0(d)-458.4(a)0(d)19.7(i)0(p)20.7(o)0(g)15.1(e)0(n)19.7(i)0(c)19.5(,)-470(ch)19.7(on)20.8(dr)20.9(og)15.1(en)19.7(ic)19.5(,)-470(a)0(n)19.7(d)-473.4(os)21(te)19.6(o-)]TJ
T*
[(ge)13.9(ni)20.7(c)-203.8(l)0(i)20.6(n)0(e)13.9(a)0(g)19.7(e)0(s)19.9(.)-205(Ma)18.8(jo)20.7(ri)20.7(ty)-198.2(of)-198(SH)20.1(ED)18.8(s)-202.5(c)0(o)19.7(e)0(x)13.9(p)0(r)20.9(e)0(s)19.9(s)0(e)19.9(d)-208.5(se)19.9(ve)19.7(ra)19.7(l)-208.6(n)0(e)19.7(u)0(r)20.9(a)0(l)]TJ
T*
[(li)14.8(ne)19.7(ag)19.7(e)-491.8(m)0(a)18.4(r)0(k)15.1(e)0(r)19.7(s)0(,)-482.4(n)0(e)19.7(s)0(t)20.9(i)0(n)15(,)-493(do)20.8(ub)20.8(le)19.6(co)19.7(rt)15(in)20.7(,)]TJ
/F8 1 Tf
16.0235 0 TD
(b)Tj
/F3 1 Tf
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[(-I)20.9(II)20.9(-t)15(ub)20.8(ul)20.7(in)20.7(,)-493(N)0(e)18.8(u)0(N)14.2(,)]TJ
-16.5649 -1.1174 TD
[(GF)14.4(AP)20.1(,)-544.8(S)0(-)21(1)0(0)20.8(0)0(,)-534.4(A)0(2)20(B)0(5)19.8(,)-544.8(an)19.7(d)-542.5(C)0(N)18.9(P)0(a)14.1(s)0(e)19.9(,)-544.8(bu)20.8(t)-542.6(n)0(o)20.8(t)-542.6(ad)13.9(en)19.7(om)19.6(at)19.5(ou)20.8(s)]TJ
0 -1.1116 TD
[(po)15.1(ly)20.7(po)20.8(si)20.9(s)-260.1(o)0(r)-255.6(m)15(ye)13.9(li)20.6(n)-260.3(b)0(a)19.7(s)0(i)20.9(c)-261.4(pr)20.9(ot)14.9(ei)19.5(n.)]TJ
/F7 1 Tf
9.9626 0 0 9.9626 313.0582 527.8676 Tm
[(Pr)-8.2(eparation)-340.1(of)-333.6(CM)]TJ
/F3 1 Tf
9.843 0 0 9.843 323.0361 510.066 Tm
[(After)-405(the)-407.8(SHEDs)-405.5(reached)-404(80%)-401.8(conuency,)-408.3(the)-402(medium)]TJ
-1.0137 -1.1174 TD
[(was)-264.3(replaced)-267(with)-259(serum-free)-268.8(DMEM)-261.7(containing)-261.4(antibiotic)]TJ
0 -1.1116 TD
[(antimycoti)-9.3(c)-405.4(solution.)-415.6(This)-408.7(cell-cultured)-416.8(CM)-407.3(was)-414.1(collected)]TJ
T*
[(after)-422.6(a)-417(48-h)-419.1(incubation.)-414.6(The)-420.4(CM)-418.8(was)-419.8(collected)-418.1(by)-416.9(centri-)]TJ
T*
[(fugation)-384.6(for)-383.4(5)-162.4(min)-384.8(at)-377.9(1,500)-169.1(rpm)-378.9(and)-378.8(was)-385.3(centrifuged)-384.4(again)]TJ
0 -1.1174 TD
[(for)-377.6(3)-168.1(min)-373.3(at)-372.1(3,000)-163.3(rpm)-378.9(to)-370.9(remove)-373(cell)-375.6(debris.)-374(We)-374.3(used)-379.7(the)]TJ
0 -1.1116 TD
[(SHED-CM)-381(without)-371.8(either)-372(enrichment)-378.8(or)-370.8(dilution.)-375.6(The)-368.5(CM)]TJ
T*
[(was)-327.7(collected)-331.7(and)-321.2(stored)-330.2(at)-320.3(4)]TJ
/F6 1 Tf
11.9341 0 TD
()Tj
/F3 1 Tf
.3974 0 TD
.3247 Tc
[(Co)324.7(r)]TJ
/F6 1 Tf
2.1484 0 TD
0 Tc
(-)Tj
/F3 1 Tf
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(80)Tj
/F6 1 Tf
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()Tj
/F3 1 Tf
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[(C)-324.7(before)-325.7(use)-326.8(in)-324.9(the)]TJ
-16.4267 -1.1174 TD
[(following)-339.5(experiments.)]TJ
/F7 1 Tf
9.9626 0 0 9.9626 313.0582 400.4786 Tm
[(SC)-340.2(migrat)-8(ion)-333.9(study)]TJ
/F3 1 Tf
9.843 0 0 9.843 323.0361 382.6204 Tm
[(SC)20(s)-219.8(\()0(c)19.7(e)0(l)19.5(l)-214.3(li)14.8(ne)-205(RT)18.6(4-)20.9(D6)20(P2)15.2(T\))-205(we)18.8(re)-204.9(pu)20.8(rc)14(ha)19.7(se)19.9(d)-214.2(f)0(r)15.1(o)0(m)-205(A)0(m)18.7(e)0(r)19.7(i)0(c)19.5(a)0(n)]TJ
-1.0137 -1.1116 TD
[(Ty)13.9(pe)-325.9(Cu)19.8(lt)14.8(ur)20.9(e)-336.3(C)0(o)19.8(l)0(l)14.8(e)0(c)18.5(t)0(i)20.6(o)0(n)-330.5(\()0(A)20(T)0(C)18.6(C)0(,)-328.1(M)0(a)18.8(n)0(a)19.7(s)0(s)15.5(a)0(s)19.9(,)-337.5(VA)19.1(\).)-327(Tr)13.9(an)19.7(sw)20.2(el)19.5(l)]TJ
T*
[(ch)13.9(am)18.5(be)19.7(rs)-209.3(wi)14.1(th)-209.7(8-)]TJ
/F8 1 Tf
6.7849 0 TD
(m)Tj
/F3 1 Tf
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[(m)-221.2(p)0(o)20.8(r)0(e)-216.4()0(l)20.9(t)0(e)19.5(r)0(s)-215.1(\()0(B)19.8(D)-220.8(Bi)19.7(oC)14.1(oa)19.7(t)-220.1(C)0(o)19.8(n)0(t)14.9(r)0(o)20.9(l)-220.1(In)15.1(se)19.9(rt)20.7(s;)]TJ
-7.3321 -1.1174 TD
[(Be)12.9(ct)19.6(on)-272.9(Di)19.8(ck)19.7(in)15(so)21(n)-283.3(a)0(n)19.7(d)-289.1(Co)19.8(.)13.9(,)-285.7(Fr)21(an)13.9(kl)20.7(in)-273(La)18.5(ke)19.7(,)-285.7(N)0(J)20.2(\))-289(we)18.8(re)-274(us)15.3(ed)-274.1(fo)20.9(r)]TJ
0 -1.1116 TD
[(mi)13.7(gr)20.9(at)19.5(io)20.7(n)-369.7(a)0(n)13.9(a)0(l)19.5(y)0(s)21(i)0(s)20.9(.)-372(A)-376.3(t)0(o)20.7(t)0(a)19.6(l)-369.8(of)-365(1.)18.5(0)]TJ
/F6 1 Tf
13.7715 0 TD
()Tj
/F3 1 Tf
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(10)Tj
7.3823 0 0 6.5614 465.2219 343.1621 Tm
(5)Tj
9.843 0 0 9.843 472.592 338.7968 Tm
[(SC)20(s)-369.5(i)0(n)-365.2(0)0(.)24.3(2)0(5)15.1(%)-370.8(FB)20(S-)]TJ
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T*
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[(vascular)-334.7(endothelial)-332.9(growth)-335.9(factor)-331.5(\(VEGF\),)-329.5(and)-332.8(hepatocyte)]TJ
T*
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T*
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T*
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T*
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[(an)19.7(al)19.5(yz)19.7(ed)-619.6(us)21(in)20.7(g)-628.9(S)0(e)19.8(q)0(u)20.8(e)0(n)19.7(c)0(e)-620.8(D)0(e)18.8(t)0(e)19.5(c)0(t)13.8(o)0(r)-612.7(s)0(o)15.3(f)0(t)20.7(w)0(a)18.8(r)0(e)19.7(,)-625.5(ve)13.9(rs)21.1(io)20.7(n)-628.9(1)0(.)24.3(1)]TJ
T*
[(\(P)21(er)19.7(ki)20.7(nE)13.9(lm)19.5(er)19.7(\).)-171.5(Th)19.6(e)-186.6(P)0(C)20(R)-186.4(co)19.7(nd)20.8(it)20.6(io)20.7(ns)-174.8(we)18.8(re)-176.1(as)-176(fo)20.9(ll)20.6(ow)14.2(s:)-169.2(on)15.1(e)-180.8(c)0(y)19.7(c)0(l)13.8(e)]TJ
0 -1.1174 TD
[(at)-199.3(50)]TJ
/F6 1 Tf
1.9065 0 TD
()Tj
/F3 1 Tf
.3859 0 TD
[(C)-215.2(f)0(o)20.9(r)-208.4(5)-156.6(m)0(i)19.5(n)-214.2(\(r)20.9(ev)19.7(er)19.7(se)-204.8(tr)20.7(an)19.7(sc)19.9(ri)15(pt)20.7(io)20.7(n\))20.9(,)-210.8(o)0(n)20.8(e)-215.4(cy)19.7(cl)19.6(e)-209.6(a)0(t)-205.1(9)0(5)]TJ
/F6 1 Tf
19.5888 0 TD
()Tj
/F3 1 Tf
.3859 0 TD
.2095 Tc
[(Cf)209.5(o)230.4(r)]TJ
-22.267 -1.1116 TD
0 Tc
[(20)-146.2(s)-306.2(\()0(i)20.7(n)0(a)19.7(c)0(t)19.5(i)0(v)20.7(a)0(t)13.8(i)0(o)20.7(n)0(/)20.7(i)0(n)20.7(i)0(t)20.6(i)0(a)13.8(l)-300.7(de)19.7(na)13.9(tu)20.7(ra)19.7(ti)20.6(on)20.8(\),)-298.2(an)19.7(d)-306.4(4)0(0)-290.2(c)0(y)13.9(c)0(l)19.6(e)0(s)-296.9(a)0(t)-291.5(9)0(5)]TJ
/F6 1 Tf
23.2404 0 TD
()Tj
/F3 1 Tf
.3859 0 TD
(C)Tj
-23.6263 -1.1116 TD
[(fo)20.9(r)-214.2(1)0(5)-146.2(s)-219.8(an)19.7(d)-214.2(6)0(0)]TJ
/F6 1 Tf
5.7309 0 TD
()Tj
/F3 1 Tf
.3859 0 TD
[(C)-215.2(f)0(o)15.1(r)-214.2(60)-146.2(s)-214(\()0(a)19.7(m)0(p)19.6(l)0(i)14.8()0(c)19.8(a)0(t)19.5(i)0(o)20.7(n)0(\))20.9(.)-216.5(Th)19.6(e)-215.4(r)0(e)14(l)0(a)19.5(t)0(i)20.6(v)0(e)-205(a)0(m)18.5(o)0(u)20.8(n)0(t)]TJ
-6.1168 -1.1174 TD
[(of)-370.8(ea)18.5(ch)-372(mR)18.6(NA)-366.8(pe)13.9(r)-375.4(s)0(a)14.1(m)0(p)19.6(l)0(e)-366.4(w)0(a)13(s)-375.3(ob)15.1(ta)19.5(in)20.7(ed)-372(by)-370.8(ca)18.5(lc)19.5(ul)20.7(at)19.5(in)20.7(g)-381.2(r)0(e)19.7(-)]TJ
0 -1.1116 TD
[(sp)21(ec)18.5(ti)20.6(ve)-205(st)20.9(an)19.7(da)19.7(rd)-203.8(cu)19.7(rv)20.9(es)14.1(.)-210.8(T)0(h)19.6(e)-209.6(st)20.9(an)19.7(da)13.9(rd)-198(cu)19.7(rv)15.1(es)-199(fo)20.9(r)-214.2(e)0(a)18.5(c)0(h)-199.2(m)0(R)18.6(N)0(A)]TJ
T*
[(we)18.8(re)-193.4(ca)18.5(lc)19.6(ul)14.9(at)19.6(ed)-193.4(us)21(in)20.7(g)-208.4(d)0(i)20.7(f)0(f)20.9(e)0(r)19.7(e)0(n)19.7(t)-202.8(co)13.9(nc)19.7(en)19.7(tr)20.7(at)19.5(io)20.7(ns)-197.8(\(2)20.9(,0)18.5(00)20.8(,)-205(4)0(0)20.8(0)0(,)-194.6(8)0(0)20.8(,)]TJ
T*
[(16)20.8(,)-182(a)0(n)13.9(d)-179.6(3.)18.5(2)-156.6(n)0(g)20.8(\))-185.4(of)-175(to)20.7(ta)13.8(l)-179.8(S)0(C)-175.9(R)0(N)18.9(A)0(.)-178.2(T)0(h)19.6(e)-186.6(re)19.7(la)19.5(ti)20.6(ve)-176.2(ex)19.7(pr)15.1(es)19.9(si)20.9(on)-175(le)19.6(ve)19.7(ls)]TJ
0 -1.1174 TD
[(we)18.8(re)-251(no)20.8(rm)19.6(al)19.5(iz)13.8(ed)-251(to)-250(GA)19.1(PD)20.1(H)-261.2(e)0(x)19.7(p)0(r)20.9(e)0(s)19.9(s)0(i)15.1(o)0(n)-249.9(l)0(e)19.5(v)0(e)19.7(l)0(s)20.9(.)]TJ
/F7 1 Tf
9.9626 0 0 9.9626 310.8472 538.866 Tm
[(Tube)-336.2(forma)-9.7(tion)-337.5(assay)]TJ
/F3 1 Tf
9.843 0 0 9.843 320.8251 521.0078 Tm
[(An)-204.7(an)19.7(gi)20.7(og)20.8(en)19.7(es)14.1(is)-203.7(as)19.9(sa)19.9(y)-214.2(k)0(i)14.9(t)-214.3(\(K)20(ur)20.9(ab)19.7(o,)-206.1(Os)20.2(ak)19.7(a,)-207.3(Ja)19.9(pa)13.9(n\))-203.8(wa)18.8(s)-214(u)0(s)21(e)0(d)]TJ
-1.0137 -1.1116 TD
[(as)-262.4(de)19.7(sc)14.1(ri)20.7(be)19.7(d)-271.8(p)0(r)15.1(e)0(v)19.7(i)0(o)20.7(u)0(s)21(l)0(y)-267.3([)0(4)20.9(2)0(])20.9(.)-274.1(Hu)20(ma)18.4(n)-271.8(u)0(m)19.6(b)0(i)14.9(l)0(i)20.6(c)0(a)18.5(l)-271.9(ve)19.7(in)-267.3(en)19.7(do)20.8(th)20.7(e-)]TJ
0 -1.1174 TD
[(li)20.6(al)-170.5(ce)18.5(ll)20.6(s)-179.5(\()0(H)14.2(U)0(V)19.1(E)0(C)18.6(s)0(\))-169(a)0(n)19.7(d)-179.6(hu)20.8(ma)18.4(n)-179.6(d)0(i)14.9(p)0(l)20.7(o)0(i)20.7(d)-179.6(b)21(ro)20.9(bl)14.9(as)19.9(ts)-169.2(we)18.8(re)-170.4(mi)19.5(xe)19.7(d)]TJ
0 -1.1116 TD
[(an)19.7(d)-243(s)0(e)14.1(e)0(d)19.7(e)0(d)-233.7(i)0(n)15(t)0(o)-232.7(t)0(h)20.7(e)-249.9(in)20.7(di)20.7(vi)20.7(du)15.1(al)-233.9(cu)19.7(lt)20.6(ur)15.1(e)-244.2(w)0(e)18.8(l)0(l)20.6(s)-248.6(of)-238.3(a)-244.2(2)0(4)20.8(-)0(w)14.3(e)0(l)19.5(l)-243.1(pl)20.7(at)13.8(e)]TJ
T*
[(un)20.8(de)19.7(r)-404.2(o)0(p)20.8(t)0(i)20.6(m)0(a)18.4(l)-398.6(tu)20.7(bu)15.1(le)-389.4(fo)20.9(rm)19.6(at)13.8(io)20.7(n)-398.5(c)0(o)19.7(n)0(d)20.8(i)0(t)14.8(i)0(o)20.7(n)0(s)21(.)-400.8(En)19.6(do)20.8(th)15(el)19.5(ia)19.5(l)-398.7(c)0(e)18.5(l)0(l)]TJ
T*
[(me)18.4(di)20.7(um)-331.7(\(E)13.9(M\))-331.4(\(K)20(ur)15.1(ab)19.7(o\))-330.5(co)13.9(nt)20.7(ai)19.5(ni)20.7(ng)-336.3(VE)18.8(GF)-331.2(\(1)15.1(0)-156.6(n)0(g)20.8(/)0(m)19.5(L)0(;)-337.6(R)0(&)24.3(D)]TJ
0 -1.1174 TD
[(Sy)21(st)20.9(em)18.5(s\))15.3(,)-187.7(H)0(G)19.1(F)-191(\(1)20.9(0)-162.4(n)0(g)20.8(/)0(m)19.5(L)0(;)-182.1(R)0(&)18.6(D)-192(Sy)21(st)20.9(em)18.5(s\))21.1(,)-193.5(o)0(r)-180.7(S)0(H)20.1(E)0(D)18.8(-)0(C)19.8(M)-192.1(wa)18.8(s)]TJ
0 -1.1116 TD
[(re)19.7(pl)20.7(ac)18.5(ed)-239.5(on)-244.1(da)19.7(ys)-238.2(1,)-240.7(4,)-240.7(7,)-234.9(an)13.9(d)-248.8(9)0(.)-240.7(A)0(f)20(t)0(e)19.6(r)-248.7(11)-238.3(da)19.7(ys)15.3(,)-245.3(t)0(h)15(e)-249.9(pl)20.7(at)19.5(es)-239.3(we)18.8(re)]TJ
T*
[(wa)18.8(sh)21(ed)-320.1(wi)19.8(th)-319.1(PB)20(S)-323.5(a)0(n)19.7(d)-329.4(x)21(ed)-320.1(wi)19.8(th)-319.1(70)20.8(%)-324.7(e)0(t)19.5(h)0(a)19.7(n)0(o)15.1(l)0(.)-315.7(A)0(f)20(t)0(e)19.5(r)-329.4(ri)20.7(ns)21(in)20.7(g)]TJ
0 -1.1174 TD
[(wi)19.8(th)-422.8(PB)14.2(S,)-424.8(th)20.7(e)-434.2(c)0(e)18.5(l)0(l)20.6(s)-438.6(we)18.8(re)-423.8(in)15(cu)19.7(ba)19.7(te)19.5(d)-433.1(w)0(i)14.1(t)0(h)-422.8(p)0(r)20.9(i)0(m)19.5(a)0(r)19.7(y)-438.8(an)19.7(ti)20.6(bo)20.8(dy)]TJ
ET
59.754 383.017 490.167 .28348 re
f
59.754 56.353 490.167 .22681 re
f
59.754 364.025 490.167 .28345 re
f
BT
/F5 1 Tf
9.845 0 0 9.845 80.9008 388.6865 Tm
(Table)Tj
/F3 1 Tf
3.0002 0 TD
(1.)Tj
/F5 1 Tf
1.2496 0 TD
[(Primer)-335.2(Sequences)-333.2(Used)-332.7(for)-332.2(Real-Time)-340.3(Reverse)-330(TranscriptasePolymerase)-337.8(Chain)-336.5(Reaction)]TJ
/F9 1 Tf
-6.3977 -1.86 TD
[(Gene)-20064.1(Sequence)-17725.9(Accession)-335.9(no.)]TJ
0 -1.9061 TD
(NGF)Tj
/F3 1 Tf
13.5959 0 TD
[(F:)-330.2(GTTGGAGATAAGACCACA)-15968(XM_227525)]TJ
0 -1.0135 TD
[(R:)-331.4(CACTGTTGTTAATGTTCAC)]TJ
T*
[(Probe:)-335.3(CCAGCACTGTCACCTCCTTG)]TJ
/F9 1 Tf
-13.5959 -1.0077 TD
(BDNF)Tj
/F3 1 Tf
13.5959 0 TD
[(F:)-330.2(GCCCTTACTATGGATAGC)-16280(NM_012513)]TJ
0 -1.0135 TD
[(R:)-331.4(CAGTGTACATACACAGGAA)]TJ
T*
[(Probe:)-335.3(TCTATCCTTATGAACCGCCAGCC)]TJ
/F9 1 Tf
-13.5959 -1.0135 TD
(NT-3)Tj
/F3 1 Tf
13.5959 0 TD
[(F:)-330.2(GGAAGATTATGTGGGCAA)-15968(NM_031073)]TJ
0 -1.0077 TD
[(R:)-331.4(GGTGACTCTTATGCTCTG)]TJ
0 -1.0135 TD
[(Probe:)-335.3(TAACCAATAGAACATCACCACGGAGG)]TJ
/F9 1 Tf
-13.5959 -1.0135 TD
(CNTF)Tj
/F3 1 Tf
13.5959 0 TD
[(F:)-330.2(CTAGCAAGGAAGATTCGTTC)-14792(NM_013166)]TJ
0 -1.0135 TD
[(R:)-331.4(CACTGAGTCAAGGTTGATA)]TJ
0 -1.0077 TD
[(Probe:)-335.3(CCTGACTGCTCTTATGGAATCTTATGT)]TJ
/F9 1 Tf
-13.5959 -1.0135 TD
(GDNF)Tj
/F3 1 Tf
13.5959 0 TD
[(F:)-330.2(CGTCTTAACTGCAATACAC)-15614(NM_019139)]TJ
0 -1.0135 TD
[(R:)-331.4(GAACCGCTACAATATCGA)]TJ
0 -1.0077 TD
[(Probe:)-335.3(TCAGTTCCTCCTTGGTTTCGTAGC)]TJ
/F9 1 Tf
-13.5959 -1.0135 TD
(Laminin)Tj
/F3 1 Tf
13.5959 0 TD
[(F:)-330.2(GGCTTTTGACATCACTTA)-16436(NM_053966)]TJ
0 -1.0135 TD
[(R:)-331.4(CGCTTATAAATGGCGAAA)]TJ
T*
[(Probe:)-335.3(CGTCTCAAGTTCCACACCAGC)]TJ
/F9 1 Tf
-13.5959 -1.0077 TD
(Fibronectin)Tj
/F3 1 Tf
13.5959 0 TD
[(F:)-330.2(GGTCACCTACAACATCATA)-15510(NM_019143)]TJ
0 -1.0135 TD
[(R:)-331.4(CAGTGTTGCCTACAGTAA)]TJ
T*
[(Probe:)-335.3(CTCTTCTCGGACCTTGTGCCT)]TJ
/F9 1 Tf
-13.5959 -1.0135 TD
[(Collagen)-338.7(IV)]TJ
/F3 1 Tf
13.5959 0 TD
[(F:)-330.2(TGGCTTCAAAGGTGATAA)-16124(NM_001135009)]TJ
0 -1.0077 TD
[(R:)-331.4(GAACCTTTATCACCAGTG)]TJ
0 -1.0135 TD
[(Probe:)-335.3(TTTGTCCTTTCTCTCCCTGGTCTC)]TJ
/F9 1 Tf
-13.5959 -1.0135 TD
(VEGF)Tj
/F3 1 Tf
13.5959 0 TD
[(F:)-330.2(ATCCCGGTTTAAATCCTG)-16384(NM_031836)]TJ
0 -1.0077 TD
[(R:)-331.4(GGAACATTTACACGTCTG)]TJ
0 -1.0135 TD
[(Probe:)-335.3(CACTGTGAGCCTTGTTCAGAGC)]TJ
/F9 1 Tf
-13.5959 -1.0135 TD
(GAPDH)Tj
/F3 1 Tf
13.5959 0 TD
[(F:)-330.2(GTTCCAGTATGACTCTACC)-15718(NM_017008)]TJ
0 -1.0135 TD
[(R:)-331.4(TCACCCCATTTGATGTTA)]TJ
0 -1.0077 TD
[(Probe:)-335.3(TTCAACGGCACAGTCAAGC)]TJ
/F4 1 Tf
8.9663 0 0 8.9663 59.7543 749.7069 Tm
[(SHED-CM)-336.8(ENHANCES)-342.9(PERIPHERAL)-334.5(NERVE)-334.5(REGENERATION)-22962.6(2689)]TJ
ET
endstream
endobj
87 0 obj
<>stream
/GS1 gs
BT
/F3 1 Tf
9.843 0 0 9.843 62.022 725.1588 Tm
0 0 0 rg
0 Tc
0 Tw
[(\(m)19.6(ou)20.8(se)-394.8(an)13.9(ti)20.6(-h)20.9(um)19.6(an)-395(CD)18.9(31)20.8(,)-406.6(1)0(:)20.7(4)0(0)20.8(0)0(;)-394(K)0(u)20(r)0(a)14(b)0(o)20.8(\))-404.2(fo)20.9(r)-404.2(1)-156.6(h)-404.3(a)0(t)-395.2(3)0(7)]TJ
/F6 1 Tf
22.9985 0 TD
()Tj
/F3 1 Tf
.3859 0 TD
(C,)Tj
-23.3844 -1.1174 TD
[(fo)20.9(ll)14.8(ow)20(ed)-360.5(by)-359.3(a)-376.6(s)0(e)19.9(c)0(o)19.7(n)0(d)20.8(a)0(r)19.7(y)-375.5(an)19.7(ti)20.6(bo)20.8(dy)-359.3(\(g)15.1(oa)19.7(t)-369.8(a)0(n)19.7(t)0(i)20.6(-)0(m)13.9(o)0(u)20.8(s)0(e)-360.3(I)0(g)20.9(G)-376.3(al)19.5(-)]TJ
0 -1.1116 TD
[(ka)19.7(li)20.6(ne)-360.5(ph)20.8(os)15.3(ph)20.8(at)19.5(as)19.9(e-)19.7(co)13.9(nj)20.7(ug)20.8(at)19.5(ed)-360.5(an)19.7(ti)20.6(bo)20.8(dy)15.1(,)-366.3(1)0(:)20.7(5)0(0)20.8(0)0(;)-359.4(K)0(u)20(r)0(a)14(b)0(o)20.8(\))-363.9(fo)15.1(r)]TJ
T*
[(1)-156.6(h)-266(at)-256.9(37)]TJ
/F6 1 Tf
3.3867 0 TD
()Tj
/F3 1 Tf
.3859 0 TD
[(C.)-259(Th)19.6(e)-261.4(r)0(e)14(a)0(c)18.5(t)0(i)20.6(o)0(n)-255.6(y)0(i)20.7(e)0(l)19.5(d)0(e)19.7(d)-266(5-)15.1(br)20.9(om)19.6(o-)20.9(4-)20.9(ch)19.7(lo)15(ro)20.9(-3)20.9(-i)20.7(nd)20.8(ol)14.9(yl)]TJ
-3.7726 -1.1116 TD
[(ph)20.8(os)15.3(ph)20.8(at)19.5(e/)19.6(ni)20.7(tr)20.7(o)-623.1(b)0(l)20.7(u)0(e)-613.9(t)0(e)19.6(t)0(r)20.7(a)0(z)18.5(o)0(l)20.7(i)0(u)20.7(m)0(.)-610.5(T)0(o)19.6(t)0(a)13.8(l)-617.5(tu)20.7(be)-613.9(le)19.6(ng)20.8(th)20.7(s)-622.9(a)0(n)19.7(d)]TJ
0 -1.1174 TD
[(nu)20.8(mb)19.6(er)19.7(s)-173.7(o)0(f)-157.7(c)0(a)12.8(p)0(i)20.7(l)0(l)20.6(a)0(r)19.7(y)-173.9(co)19.7(nn)20.8(ec)18.5(ti)20.6(on)20.8(s)-173.7(\()0(j)20.7(o)0(i)20.7(n)0(t)20.7(s)0(\))-163.2(i)0(n)-163.6()0(v)21(e)-169.3(di)20.7(ff)15.1(er)19.7(en)19.7(t)-168.3()0(e)14.1(l)0(d)20.7(s)]TJ
0 -1.1116 TD
[(\(5)20.9(.5)-148.5(mm)]TJ
7.3823 0 0 6.5614 94.0535 663.7605 Tm
(2)Tj
9.843 0 0 9.843 100.2897 659.3951 Tm
[(pe)19.7(r)-260.2()0(e)19.8(l)0(d)20.7(\))-260.2(pe)19.7(r)-260.3(w)0(e)18.8(l)0(l)-250.1(w)0(e)18.8(r)0(e)-251(m)0(e)18.4(a)0(s)19.9(u)0(r)15.1(e)0(d)-251(q)0(u)20.8(a)0(n)19.7(t)0(i)20.6(t)0(a)19.6(t)0(i)20.6(v)0(e)13.9(l)0(y)-244.2(u)0(s)15.3(-)]TJ
-3.8878 -1.1116 TD
[(in)20.7(g)-191.2(t)0(h)20.7(e)-192.3(An)20(gi)20.7(og)20.8(en)19.7(es)14.1(is)-174.9(Im)19.6(ag)13.9(e)-186.6(A)0(n)20(a)0(l)19.6(y)0(z)19.7(e)0(r)14(,)-187.7(ve)19.7(rs)21.1(io)15(n)-185.4(2)-191.2(\(K)20(ur)20.9(ab)19.7(o\))15.1(,)-187.7(a)0(n)19.7(d)]TJ
0 -1.1174 TD
[(th)20.7(e)-261.4(r)0(e)19.7(s)0(u)15.3(l)0(t)20.6(s)-260.1(we)18.8(re)-251(su)21(bj)14.9(ec)18.5(te)19.5(d)-260.3(t)0(o)-250(s)0(t)20.9(a)0(t)19.5(i)0(s)15.2(t)0(i)20.6(c)0(a)18.5(l)-260.4(an)19.7(al)19.5(ys)21(is)15.1(.)]TJ
/F7 1 Tf
9.9626 0 0 9.9626 62.022 615.5715 Tm
[(DRG)-338(neur)-8.4(ite)-331.8(gro)-8.4(wth)-332.6(as)-6.4(say)-331.6(and)-341.4(cell)-333(v)-6.5(iability)-338.6(assa)-7.2(y)]TJ
/F3 1 Tf
9.843 0 0 9.843 72 597.7133 Tm
[(DR)18.9(G)-439.7(n)0(e)19.7(u)0(r)20.9(o)0(n)20.8(s)-438.6(we)18.8(re)-429.5(ha)19.7(rv)20.9(es)14.1(te)19.6(d)-438.8(f)0(r)20.9(o)0(m)-429.6(3)0(-)20.9(d)0(a)19.7(y)0(-)20.9(o)0(l)20.7(d)-438.8(Wi)18.4(st)20.9(ar)14(/S)20.9(T)]TJ
-1.0137 -1.1116 TD
[(ra)19.7(ts)-342(us)21(in)20.7(g)-352.4(a)-347.8(pr)15.1(ev)19.7(io)20.7(us)21(ly)-342.1(de)19.7(sc)19.9(ri)15(be)19.7(d)-346.7(p)0(r)20.9(o)0(t)14.9(o)0(c)19.7(o)0(l)-336.4([)0(4)15.1(3)0()20.8(4)0(5)20.8(])0(.)-338.6(N)0(e)18.8(u)0(r)15.1(o)0(n)20.8(s)]TJ
T*
[(we)18.8(re)-331.6(re)14(su)21(sp)21(en)19.7(de)13.9(d)-340.9(i)0(n)-330.6(n)0(e)19.7(u)0(r)20.9(o)0(b)15.1(a)0(s)19.9(a)0(l)-331.8(m)0(e)18.4(d)0(i)20.7(u)0(m)-331.7(\()0(G)20(i)0(b)15(c)0(o)19.7(\))-340.9(co)19.7(nt)20.7(ai)19.5(ni)15(ng)]TJ
0 -1.1174 TD
.1566 Tc
[(2m)156.6(M)]TJ
/F5 1 Tf
2.6034 0 TD
0 Tc
(l)Tj
/F3 1 Tf
.4896 0 TD
[(-g)15.1(lu)20.7(ta)19.6(mi)19.5(ne)-279.8(\(G)20(ib)15(co)19.7(\),)-281(2%)-279.7(B2)19.8(7)-289.1(\()0(G)20(i)0(b)15(c)0(o)19.7(\))0(,)-281(a)0(n)19.7(d)-289.1(an)19.7(ti)20.6(bi)20.7(ot)14.9(ic)19.6()]TJ
-3.093 -1.1116 TD
[(an)19.7(ti)20.6(my)19.6(co)13.9(ti)20.6(c)-520.6(s)0(o)15.3(l)0(u)20.7(t)0(i)20.6(o)0(n)20.8(,)-521.8(an)19.7(d)-525.2(t)0(h)20.7(e)0(n)-516(s)0(e)19.9(e)0(d)19.7(e)0(d)-516(o)0(n)20.8(t)0(o)-509.2(p)0(o)15.1(l)0(y)20.7(-)]TJ
/F5 1 Tf
20.8098 0 TD
(d)Tj
/F3 1 Tf
.5414 0 TD
[(-l)20.7(ys)15.3(in)20.7(e/)]TJ
-21.3512 -1.1116 TD
[(la)19.5(mi)19.5(ni)20.7(n-)15.1(co)19.7(at)19.5(ed)-377.7(24)20.8(-w)20(el)19.5(l)-387.1(p)0(l)20.7(a)0(t)19.5(e)0(s)-377.5(a)0(t)-372.1(a)-388.2(de)19.7(ns)21(it)20.6(y)-387(o)0(f)-370.8(4)0(0)15.1(0)0()20.8(5)0(0)20.8(0)-381.2(ne)13.9(u-)]TJ
0 -1.1174 TD
[(ro)20.9(ns)-278.5(pe)19.7(r)-283.3(w)0(e)13(l)0(l)20.6(.)-285.7(DR)18.9(G)-284.2(n)0(e)19.7(u)0(r)15.1(o)0(n)20.8(s)-283.1(we)18.8(re)-279.8(in)20.7(cu)19.7(ba)19.7(te)19.6(d)-289.1(w)0(i)19.8(t)0(h)-273(S)0(H)14.4(E)0(D)18.8(-)0(C)19.8(M)]TJ
0 -1.1116 TD
[(or)-336.2(DM)13.3(EM)-338.4(\()]TJ
/F6 1 Tf
4.9188 0 TD
(-)Tj
/F3 1 Tf
.5414 0 TD
[(\))-346.6(f)0(o)15.1(r)-346.6(48)-146.2(h.)-338.6(Th)19.6(e)-347.8(n)0(e)19.7(u)0(r)20.9(o)0(n)15.1(s)-346.5(we)18.8(re)-337.4(th)15(en)-337.4(x)21(ed)-337.4(in)-342.1(4%)]TJ
-5.4602 -1.1116 TD
[(pa)19.7(ra)19.7(fo)15.1(rm)19.7(al)19.5(de)19.7(hy)20.8(de)-383.5(fo)20.9(r)-392.7(1)0(5)-146.2(m)0(i)19.5(n)-387(an)19.7(d)-392.8(i)0(n)20.7(c)0(u)19.7(b)0(a)19.7(t)0(e)13.8(d)-387(wi)19.8(th)-382.5(a)-388.2(p)0(r)15.1(i)0(m)19.5(a)0(r)19.7(y)]TJ
0 -1.1174 TD
[(an)19.7(ti)20.6(bo)15.1(dy)-543.6(ag)13.9(ai)19.5(ns)21(t)-559.9(m)0(o)19.6(n)0(o)20.8(c)0(l)19.5(o)0(n)20.8(a)0(l)]TJ
/F8 1 Tf
12.5504 0 TD
(b)Tj
/F3 1 Tf
.5414 0 TD
[(-I)20.9(II)15.1(-t)20.7(ub)20.8(ul)20.7(in)-549.5(\(1)20.9(:5)15(00)20.8(;)-554.2(S)0(i)15.1(g)0(m)19.6(a)0(-)]TJ
-13.0918 -1.1116 TD
[(Al)19.8(dr)15.1(ic)19.5(h,)-401.9(St)15.1(.)-412.4(L)0(o)19.6(u)0(i)20.7(s)0(,)-407.5(M)0(O)19.1(\))-415.8(ov)20.8(er)14(ni)20.7(gh)20.8(t)-415.9(a)0(t)-406.7(4)]TJ
/F6 1 Tf
16.1733 0 TD
()Tj
/F3 1 Tf
.3859 0 TD
[(C,)-408.7(fo)20.9(ll)20.6(ow)20(ed)-406.5(by)-411.1(in)20.7(-)]TJ
-16.5592 -1.1116 TD
[(cu)19.7(ba)19.7(ti)14.8(on)-232.6(wi)19.8(th)-238.5(an)-233.7(Al)19.8(ex)13.9(a)-244.2(F)0(l)20.9(u)0(o)20.8(r)-248.7(63)20.8(3-)20.9(co)19.7(nj)14.9(ug)20.8(at)19.5(ed)-233.7(an)13.9(ti)20.6(-m)19.6(ou)20.8(se)-239.3(Ig)20.9(G)]TJ
T*
[(se)19.9(co)19.7(nd)15.1(ar)19.7(y)-277.6(a)0(n)13.9(t)0(i)20.6(b)0(o)20.8(d)0(y)-272.9(\()0(1)20.9(:)0(5)20.7(0)0(0)20.8(;)-283.5(In)20.9(vi)20.7(tr)15(og)20.8(en)19.7(,)-279.9(C)0(a)18.6(r)0(l)20.7(s)0(b)15.3(a)0(d)19.7(,)-279.9(CA)18.9(\))-283.3(f)0(o)20.9(r)-283.3(1)-156.6(h)]TJ
0 -1.1174 TD
[(at)-470(ro)20.9(om)-470(te)13.8(m)15(p)0(e)13.9(r)0(a)19.7(t)0(u)20.7(r)0(e)19.7(.)-481.5(Th)19.6(e)-480.3(s)0(t)20.9(a)0(i)19.5(n)0(e)19.7(d)-479.2(ne)13.9(ur)20.9(on)20.8(s)-479(w)0(e)18.8(r)0(e)-469.9(e)0(x)13.9(a)0(m)18.4(i)0(n)20.7(e)0(d)]TJ
0 -1.1116 TD
[(un)20.8(de)19.7(r)-323.6(a)-324.8(u)21(or)15.1(es)19.9(ce)18.5(nc)19.7(e)-324.8(m)0(i)19.5(c)0(r)19.7(o)0(s)21(c)0(o)13.9(p)0(e)-308.6(\()0(B)14.1(Z)0(9)19.6(0)0(0)20.8(0)0(;)-313.4(K)0(e)18.8(y)0(e)19.7(n)0(c)19.7(e)0(,)-316.7(O)0(s)20.2(a)0(k)19.7(a)0(,)]TJ
T*
[(Ja)19.9(pa)19.7(n\))15.1(.)-435.4(T)0(h)19.6(e)-434.2(av)19.7(er)14(ag)19.7(e)-434.2(n)0(e)19.7(u)0(r)15.1(i)0(t)20.6(e)-434.2(le)19.6(ng)15.1(th)-422.8(wa)18.8(s)-438.6(q)0(u)20.8(a)0(n)19.7(t)0(i)20.6(t)0(a)13.8(t)0(i)20.6(v)0(e)19.7(l)0(y)-428.5(a)0(n)19.7(a)0(-)]TJ
0 -1.1174 TD
[(ly)20.7(ze)18.5(d)-340.9(u)0(s)21(i)0(n)20.7(g)-335.2(BZ)18.6(90)20.8(00)-330.5(an)19.7(al)19.5(ys)21(is)-330.4(so)21(ft)20.7(wa)18.8(re)-331.6(\(K)20(ey)19.7(en)19.7(ce)18.5(\).)-327(A)-336(C)0(C)13(K)0(-)20(8)]TJ
0 -1.1116 TD
[(as)19.9(sa)14.1(y)-271.8(w)0(a)18.8(s)-277.4(pe)19.7(rf)15.1(or)20.9(me)18.4(d)-277.6(t)0(o)-261.5(a)0(s)14.1(s)0(e)19.9(s)0(s)-266.8(c)0(e)18.5(l)0(l)-267.4(v)0(i)20.7(a)0(b)19.7(i)0(l)20.6(i)0(t)14.8(y)0(.)-263.7(A)0(b)20(s)0(o)21(r)0(b)15.1(a)0(n)19.7(c)0(e)-269.5(w)0(a)18.8(s)]TJ
T*
[(me)18.4(as)19.9(ur)15.1(ed)-251(at)-256.9(45)20.8(0)-156.6(n)0(m)-251.1(u)0(s)15.3(i)0(n)20.7(g)-260.3(a)-267.2(m)0(i)19.5(c)0(r)19.7(o)0(p)20.8(l)0(a)19.6(t)0(e)-256.9(r)0(e)19.7(a)0(d)19.7(e)0(r)-256.8(\()0(P)21(o)0(w)20(e)0(r)14(S)0(c)19.8(a)0(n)19.7(4)0(\))20.9(.)]TJ
/F7 1 Tf
9.9626 0 0 9.9626 62.022 378.5385 Tm
[(Sciat)-8(ic)-334.7(nerve)-339.2(transe)-7(ction)-338.2(and)-335.7(implant)-9.1(ation)]TJ
/F3 1 Tf
9.843 0 0 9.843 72 360.7369 Tm
[(Ma)18.8(le)-303(Wi)18.4(st)20.9(ar)19.7(/S)20.9(T)-313.3(r)0(a)19.7(t)0(s)-301.6(w)0(e)18.8(i)0(g)15(h)0(i)20.7(n)0(g)-301.7(2)0(5)20.8(0)0()20.8(3)0(0)20.8(0)-156.6(g)-312.1(\()0(7)20.9()0(8)-301.7(w)0(e)18.8(e)0(k)13.9(s)-306.2(ol)14.9(d\))]TJ
-1.0137 -1.1174 TD
[(we)18.8(re)-354.7(an)19.7(es)19.9(th)20.7(et)19.5(iz)19.6(ed)-354.7(us)21(in)20.7(g)-364(p)0(e)19.7(n)0(t)20.7(o)0(b)20.8(a)0(r)19.7(b)0(i)20.7(t)0(a)13.8(l)-358.3(\(2)20.9(0)-156.6(m)0(g)19.6(/)0(k)20.7(g)-364(in)20.7(tr)20.7(ap)19.7(er)19.7(it)14.8(o-)]TJ
0 -1.1116 TD
[(ne)19.7(al)19.5(ly)15(;)-536.9(K)0(y)20(o)0(r)15.1(i)0(t)20.6(s)0(u)-531.9(S)0(e)19.8(i)0(y)20.7(a)0(k)19.7(u)0(,)-528.7(T)0(o)19.6(k)0(y)15.1(o)0(,)-528.7(J)0(a)19.9(p)0(a)19.7(n)0(\))20.9(.)-544.8(Th)19.6(e)-537.9(l)0(e)19.5(f)0(t)-532.2(s)0(c)19.9(i)0(a)13.8(t)0(i)20.6(c)]TJ
T*
[(ne)19.7(rv)20.9(es)-343(we)18.8(re)-343.2(ca)18.5(re)19.7(fu)15.1(ll)20.6(y)-346.7(e)0(x)13.9(p)0(o)20.8(s)0(e)19.9(d)-352.4(an)19.7(d)-346.7(i)0(s)15.1(o)0(l)20.7(a)0(t)19.5(e)0(d)-343.2(a)0(t)-337.6(t)0(h)15(e)-347.8(mi)19.5(d-)20.9(th)15(ig)20.7(h)]TJ
0 -1.1174 TD
[(le)19.5(ve)19.7(l.)-165.9(Us)20.2(in)15(g)-173.9(a)-175(mi)19.5(cr)19.7(os)21(co)19.7(pe)-164.6(an)13.9(d)-173.9(m)0(i)19.5(c)0(r)19.7(o)0(s)21(u)0(r)20.9(g)0(i)14.9(c)0(a)18.5(l)-174(in)20.7(st)20.9(ru)15.1(m)15(e)0(n)13.9(t)0(s)20.9(,)-176.2(a)-175(1)0(2)20.8(-)]TJ
0 -1.1116 TD
[(mm)-304.2(se)19.9(gm)19.6(en)13.9(t)-312.3(o)0(f)-301.7(t)0(h)15(e)-313.3(ne)19.7(rv)20.9(e)-313.3(w)0(a)13(s)-311.9(ex)19.7(ci)19.5(se)19.9(d)-317.9(a)0(n)19.7(d)-312.1(th)20.7(e)-319(n)0(e)19.7(r)0(v)20.9(e)-313.3(st)15.1(um)19.6(ps)]TJ
T*
[(we)18.8(re)-458.3(pu)20.8(ll)20.6(ed)-452.6(1)-156.6(m)0(m)-453.9(i)0(n)15(s)0(i)20.9(d)0(e)-452.6(e)0(a)18.5(c)0(h)-458.4(e)0(n)19.7(d)-461.9(of)-451.4(th)20.7(e)-468.8(1)0(2)20.8(-)0(m)19.6(m)-463.1(si)20.9(li)14.8(co)19.7(ne)]TJ
0 -1.1174 TD
[(co)19.7(nd)20.8(ui)15(t)-168.3(\()0(1)15.1(.)13.9(6)-156.6(m)0(m)-165.9(i)0(n)20.7(n)0(e)19.7(r)-173.9(di)15(am)24.2(et)13.8(er)19.7(\))-173.9(a)0(n)19.7(d)-173.9(se)19.9(cu)19.7(re)19.7(d)-173.9(t)0(o)-163.6(t)0(h)20.7(e)-175(ep)19.7(in)15(eu)19.7(ri)20.7(um)]TJ
0 -1.1116 TD
[(wi)19.8(th)-192.4(8)20.8(0)-196.9(n)0(y)20.8(l)0(o)20.7(n)-196.9(su)15.3(tu)20.7(re)19.7(s)-196.7(\()0(N)14.2(a)0(t)19.5(s)0(u)21(m)0(e)18.4(,)-199.3(To)19.6(ky)20.8(o,)-188.8(Ja)19.9(pa)19.7(n;)-192.4(Fi)20.9(g.)-188.8(5A)20(\).)-188.8(Th)19.6(e)]TJ
T*
[(mu)19.6(sc)19.9(le)-343.3(an)19.7(d)-346.7(s)0(k)21(i)0(n)-342.1(l)0(a)19.5(y)0(e)19.7(r)0(s)-341.8(w)0(e)18.8(r)0(e)-337.4(c)0(l)19.5(o)0(s)15.3(e)0(d)-337.4(w)0(i)19.8(t)0(h)-342.1(4)0()20.8(0)-346.7(Vi)19.8(cr)14(yl)-336.4(su)21(tu)20.7(re)14(s)]TJ
0 -1.1174 TD
[(\(E)19.7(th)20.7(ic)13.8(on)-365.1(In)20.9(c.)17.3(,)-377.8(S)0(o)21(m)0(e)18.4(r)0(v)20.9(i)0(l)14.8(l)0(e)19.5(,)-377.8(NJ)20.2(\).)-367.4(Th)19.6(e)-382.4(a)0(n)19.7(i)0(m)19.5(a)0(l)19.5(s)-381(we)18.8(re)-366.2(ra)19.7(nd)15.1(om)19.6(ly)]TJ
0 -1.1116 TD
[(as)19.9(si)15.1(gn)20.8(ed)-245.3(to)-250(th)20.7(e)-261.4(f)0(o)20.9(l)0(l)20.6(o)0(w)14.2(i)0(n)20.7(g)-254.5(v)15.2(e)-255.7(g)0(r)20.9(o)0(u)15.1(p)0(s)21(:)-260.4(\(1)20.9(\))-254.5(S)0(h)15.2(a)0(m)18.5(:)-254.7(sc)14.1(ia)19.6(ti)20.6(c)-255.7(n)0(e)13.9(r)0(v)20.9(e)]TJ
T*
[(ex)19.7(po)20.8(su)15.3(re)-308.6(wi)19.8(th)20.7(ou)20.8(t)-318(a)0(n)19.7(y)-317.9(da)19.7(ma)18.4(ge)-308.6(to)-307.6(th)20.7(e)-319(n)0(e)19.7(r)0(v)15.1(e)-313.3(ti)14.8(ss)21.2(ue)19.7(;)-318(\()0(2)20.9(\))-317.8(Tr)19.7(an)13.9(s-)]TJ
T*
[(ec)18.5(ti)20.6(on)15.1(:)-565.7(u)0(n)20.8(r)0(e)19.7(p)0(a)19.7(i)0(r)20.7(e)0(d)-556.3(s)0(c)14.1(i)0(a)19.6(t)0(i)20.6(c)-566.7(ne)19.7(rv)20.9(e)-566.7(d)0(e)19.7(f)0(e)14(c)0(t)19.5(s)0(;)-555.1(\()0(3)20.9(\))-565.5(DM)19.1(EM)-557.2(\()]TJ
/F6 1 Tf
23.1425 0 TD
(-)Tj
/F3 1 Tf
.5472 0 TD
(\):)Tj
-23.6897 -1.1174 TD
[(DM)19.1(EM)-200.1(\()]TJ
/F6 1 Tf
3.6056 0 TD
(-)Tj
/F3 1 Tf
.5414 0 TD
[(\)-)20.9(l)15.1(le)19.6(d)-202.7(s)0(i)15.1(l)0(i)20.6(c)0(o)19.7(n)-208.4(co)19.7(nd)20.8(ui)20.7(t;)-198.3(\(4)20.9(\))-208.4(S)0(H)20.1(E)0(D)18.8(-)0(C)14.1(M)0(:)-193.3(S)0(H)20.1(E)0(D)13(-)0(C)19.8(M)0(-)]TJ
-4.147 -1.1116 TD
[(l)20.9(le)19.5(d)-369.7(s)0(i)20.9(l)0(i)20.6(c)0(o)19.7(n)-364(co)19.7(nd)15.1(ui)20.7(t;)-353.8(an)19.7(d)-364(\()0(5)15.1(\))-363.9(Au)20(to)20.7(gr)20.9(af)14(t:)-353.8(th)20.7(e)-365.1(r)0(e)19.7(m)0(o)19.6(v)0(e)19.7(d)-364(ne)13.9(rv)20.9(e)]TJ
T*
[(se)19.9(gm)19.6(en)19.7(t)-260.4(w)0(a)18.8(s)-254.3(ro)20.9(ta)19.6(te)19.6(d)-260.3(1)0(8)20.8(0)]TJ
/F6 1 Tf
9.7857 0 TD
()Tj
/F3 1 Tf
.6508 0 TD
-.0189 Tc
[(a)-18.9(n).8(d)-273.4(r)-18.9(ei)-18.9(mp)-18.9(la)-18.9(nt)-18.9(ed)-18.9(.)-265.3(A)-18.9(ft)-18.9(er)-273.4(t)-18.9(h)1.8(e)-274.6(s)-18.9(ur)-18.9(ge)-18.9(ry)-18.9(,)]TJ
-10.4366 -1.1174 TD
0 Tc
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T*
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()Tj
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(C)Tj
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()Tj
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()Tj
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T*
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T*
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T*
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T*
[(value)-342(near)]TJ
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(-)Tj
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[(100)-337.4(indicates)-341.9(complete)-342.1(dysfunction,)-339.3(whereas)-340.1(a)]TJ
-5.1377 -1.1174 TD
[(value)-336.3(near)-335(0)-335.2(indicates)-336.1(normal)-337.4(motor)-335.1(function)-332.7([47].)]TJ
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9.9626 0 0 9.9626 313.0582 582.6896 Tm
[(Ele)-9.4(ctrophys)-8.5(iologic)-336.8(test)-7.8(ing)]TJ
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[(At)-464(12)-457.2(weeks)-463.5(after)-462.9(surgery,)-461.3(electromyography)-461.4(was)-465.9(per-)]TJ
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T*
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[(trical)-786.9(stimuli)-786.8(were)-785.1(applied)-782.1(to)-785.6(the)-782.2(sciatic)-789(nerve)-785.4(and)]TJ
T*
[(compound)-258.9(muscle)-252(action)-256.9(potentials)-261.3(\(CMAPs\))-252(were)-255.2(recorded)]TJ
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T*
[(computer)-329.3(and)-321.2(the)-327.1(latency,)-325.9(amplitude,)-322.6(and)-327(nerve)-324.6(conduction)]TJ
T*
[(veloc)-8(ity)-331.9(of)-330.5(the)-338.7(CMPAs)-336.3(were)-335.9(calculated)-339.8(from)-333.9(these)-336.1(data.)]TJ
/F7 1 Tf
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[(trichr)-8(ome)-339.2(staining)]TJ
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-1.0137 -1.1116 TD
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T*
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T*
[(trocnemius)-625.1(muscle)-620.6(wet)-621.8(weight)-619.4(ratio)-623.2(was)-621.4(calculated)-622(by)]TJ
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[(injured)-366.2(side)-362.6(to)-359.4(that)-362.9(on)-359.3(the)-361.7(normal)-366.2(side.)-360.3(The)-362.8(gastrocnemius)]TJ
T*
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T*
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T*
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[(Hi)-8.6(stomorpho)-10.4(logical)-336.8(analys)-11.1(is)]TJ
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[(At)-325.7(12)-324.7(weeks)-325.3(after)-330.4(surgery,)-323.1(distal)-324.7(regenerating)-329.1(nerve)-330.3(seg-)]TJ
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[(ments)-330.4(were)-330.1(harvested)-330.1(and)-321.2(xed)-328(in)-324.9(2.5%)-324.6(glutaraldehyde)-331.5(so-)]TJ
T*
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[(subsequently)-326.4(xed)-316.4(in)-319.1(2%)-320(osmium)-320(tetroxide)-317.9(\(OsO)]TJ
7.3823 0 0 6.5614 512.0503 186.8031 Tm
(4)Tj
9.843 0 0 9.843 515.7353 188.4472 Tm
[(\))-317.8(\(TAAB)]TJ
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[(Labor)-7.8(atories)-286.5(Equipment)-290.2(Ltd.\))-285.6(for)-285.5(1)-168.1(h)0(,)-286.8(separately)-292.2(dehydrated)]TJ
T*
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[(66%,)-457.1(and)-459.5(100%;)-456(TAAB)-457.6(Laboratories)-461.4(Equipment)-457.3(Ltd.\))-452.6(in)]TJ
T*
[(propyl)-6.7(ene)-357(oxide)-352.4(\(Nacalai)-360.1(Tesque,)-359(Inc.,)-356.9(Kyoto,)-361.3(Japan\).)-357.7(Tis-)]TJ
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[(sues)-333.5(were)-330.1(embedded)-336.1(in)-330.6(EPON812)-327.3(in)-330.6(a)-330.6(6)0(0)]TJ
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()Tj
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[(number)-233.6(of)-232.6(myelinated)-235(axons)-236.9(was)-229.8(counted)-234.8(using)-235.8(a)-232.6(uorescent)]TJ
T*
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(2690)Tj
39.2969 0 TD
[(SUGIMURA-WAKAYAMA)-338.1(ET)-338.7(AL.)]TJ
ET
endstream
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[(lead)-508(citrate)-501.1(\(Sigma-Aldrich\))-510.6(and)-499.8(2%)-504.4(uranyl)-503.2(acetate.)-506.8(The)]TJ
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T*
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T*
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[(All)-378.8(histological)-384.6(and)-378.8(functional)-382.3(analyses)-380.7(were)-382(performed)]TJ
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T*
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(-)Tj
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[(\),)-234.9(SHED-CM,)-240.5(and)-234.9(15%)-234.8(FBS)-240.2(were)]TJ
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[(17,)-322.4(respectively.)-321(SHED-CM)]TJ
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T*
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[(0.05;)-330.5(Fig.)-335(1A\).)]TJ
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[(MT)18.7(T)-417(a)0(s)19.9(s)0(a)14.1(y)0(s)-405.2(w)0(e)18.8(r)0(e)-412.3(p)0(e)19.7(r)0(f)20.9(o)0(r)20.9(m)0(e)18.4(d)-415.8(to)-411.3(co)19.7(n)21(rm)-406.6(th)20.7(at)-412.4(SH)20.1(ED)18.8(-C)19.8(M)]TJ
-1.0137 -1.1116 TD
[(en)13.9(ha)19.7(nc)19.7(ed)-233.7(SC)-239.2(pr)20.9(ol)20.7(if)20.7(er)14(at)19.5(io)20.7(n.)-234.9(SH)20.1(ED)18.8(-C)19.8(M)-249.7(s)0(i)20.9(g)0(n)20.8(i)0()15.1(c)0(a)18.5(n)0(t)20.7(l)0(y)-232.7(i)0(n)14.9(c)0(r)19.7(e)0(a)18.5(s)0(e)19.9(d)]TJ
T*
[(th)15(e)-428.5(S)0(C)-423.5(p)0(r)20.9(o)0(l)20.7(i)0(f)20.7(e)0(r)19.7(a)0(t)13.8(i)0(o)20.7(n)-427.3(ra)19.7(te)-423.9(wh)20(en)-423.8(co)19.7(mp)19.6(ar)19.7(ed)-423.8(wi)19.8(th)-417(DM)19.1(EM)-424.8(\()]TJ
/F6 1 Tf
23.419 0 TD
(-)Tj
/F3 1 Tf
.5414 0 TD
(\))Tj
-23.9604 -1.1174 TD
(\(*)Tj
/F9 1 Tf
.8179 0 TD
(P)Tj
/F6 1 Tf
.7718 0 TD
(<)Tj
/F3 1 Tf
.7084 0 TD
[(0.)24.3(05)15.1(;)-260.4(F)0(i)20.9(g)0(.)-246.4(1)0(B)14.1(\))0(.)]TJ
/F7 1 Tf
9.9626 0 0 9.9626 59.7543 392.0314 Tm
[(Gr)-7(owth)-338.2(factors)-335.6(pre)-8.4(sent)-334.3(in)-334(SHE)-10(D-CM)]TJ
/F3 1 Tf
9.843 0 0 9.843 69.7323 374.1731 Tm
[(The)-616.2(concentrations)-625(of)-612.7(growth)-618.1(factors,)-618.1(such)-615.9(as)-619.5(NGF,)]TJ
-1.0137 -1.1116 TD
[(BDNF,)-402.1(NT-3,)-401.5(GDNF,)-402(CNTF,)-402.4(VEGF,)-402.3(and)-401.9(HGF)-402.3(in)-399.7(SHED-)]TJ
T*
[(CM,)-249.5(were)-249.5(quantied)-254.2(through)-244(ELISA.)-253.9(These)-250.7(factors)-246.1(were)-249.5(not)]TJ
0 -1.1174 TD
[(detected)-244.1(in)-238.5(DMEM)-238.7(\()]TJ
/F6 1 Tf
8.2882 0 TD
(-)Tj
/F3 1 Tf
.5529 0 TD
[(\),)-240.6(whereas)-242.2(SHED-CM)-237(conta)-8(ined)-236.1(NGF,)]TJ
-8.8411 -1.1116 TD
[(BDNF,)-269.6(NT-3,)-263.3(GDNF,)-269.5(CNTF,)-264.2(VEGF,)-269.8(and)-263.7(HGF)-264.1(at)-268.4(respective)]TJ
T*
[(concentrations)-371.6(of)-359.3(58.7)]TJ
/F6 1 Tf
9.3307 0 TD
()Tj
/F3 1 Tf
.72 0 TD
[(28,)-362.7(46.4)]TJ
/F6 1 Tf
3.5307 0 TD
()Tj
/F3 1 Tf
.7142 0 TD
[(5.3,)-366.2(24.9)]TJ
/F6 1 Tf
3.7841 0 TD
()Tj
/F3 1 Tf
.7142 0 TD
[(2.1,)-366.2(115)]TJ
/F6 1 Tf
3.5307 0 TD
()Tj
/F3 1 Tf
.72 0 TD
(50,)Tj
-23.0446 -1.1174 TD
(131)Tj
/F6 1 Tf
1.6703 0 TD
()Tj
/F3 1 Tf
.72 0 TD
[(71,)-328.2(556)]TJ
/F6 1 Tf
3.2485 0 TD
()Tj
/F3 1 Tf
.72 0 TD
[(65,)-333.9(and)-332.8(3,350)]TJ
/F6 1 Tf
5.777 0 TD
()Tj
/F3 1 Tf
.72 0 TD
[(560)-170.3(pg/mL)-330.6(\(Table)-338.5(2\).)]TJ
/F7 1 Tf
9.9626 0 0 9.9626 310.8472 725.0455 Tm
[(SHED)-9(-CM)-335.3(enhanc)-6.3(es)-336.5(the)-339.2(expressio)-8.9(n)-330(o)0(f)-339.2(NTF,)]TJ
0 -1.2007 TD
[(angioge)-9.5(nic)-334.7(molec)-8.1(ule,)-337.5(and)-335.7(ECM)-338(mark)-7(er)-332.8(genes)]TJ
/F3 1 Tf
9.843 0 0 9.843 320.8251 695.2817 Tm
[(The)-426.1(effects)-425.8(of)-428.4(SHED-CM)-427.1(and)-430.7(DMEM)-428.7(\()]TJ
/F6 1 Tf
16.9969 0 TD
(-)Tj
/F3 1 Tf
.5529 0 TD
[(\))-427.3(o)0(n)-428.4(S)0(C)-423.5(gene)]TJ
-18.5635 -1.1116 TD
[(expression)-243.4(were)-238(measured)-243.7(using)-241.6(real-time)-245.3(RT-PCR)-241.1(analysis.)]TJ
0 -1.1174 TD
[(The)-639.2(expression)-640.9(levels)-636.8(of)-635.7(genes)-641.2(encoding)-639.1(NTFs)-639.7(\()]TJ
/F9 1 Tf
22.0424 0 TD
(NGF)Tj
/F3 1 Tf
1.9986 0 TD
(,)Tj
/F9 1 Tf
-24.041 -1.1116 TD
(BDNF)Tj
/F3 1 Tf
2.6149 0 TD
(,)Tj
/F9 1 Tf
1.1692 0 TD
(NT-3)Tj
/F3 1 Tf
2.0505 0 TD
(,)Tj
/F9 1 Tf
1.1692 0 TD
(CNTF)Tj
/F3 1 Tf
2.4997 0 TD
(,)Tj
/F9 1 Tf
1.1692 0 TD
(GDNF)Tj
/F3 1 Tf
2.7186 0 TD
[(\),)-914.5(angiogenic)-924.8(molecules)]TJ
-13.3913 -1.1116 TD
(\()Tj
/F9 1 Tf
.3341 0 TD
(VEGF)Tj
/F3 1 Tf
2.5631 0 TD
[(\),)-234.9(and)-240.6(ECM)-236.8(molecules)]TJ
/F9 1 Tf
9.2213 0 TD
[(\(laminin,)-238.1(bronectin,)-241.5(collagen)]TJ
-12.1184 -1.1174 TD
[(type)-277.6(IV)]TJ
/F3 1 Tf
2.9029 0 TD
[(\))-277.5(were)-284(signicantly)-280.7(upregulated)-280.8(in)-278.8(SCs)-280.4(cultured)-282.1(with)]TJ
-2.9029 -1.1116 TD
[(SHED-CM)-646(relative)-640.5(to)-641.7(those)-645.9(cultured)-644.9(with)-639.1(DMEM)-647.6(\()]TJ
/F6 1 Tf
23.4074 0 TD
(-)Tj
/F3 1 Tf
.5472 0 TD
(\))Tj
-23.9546 -1.1116 TD
(\(*)Tj
/F9 1 Tf
.8294 0 TD
(P)Tj
/F6 1 Tf
.7833 0 TD
(<)Tj
/F3 1 Tf
.72 0 TD
[(0.05;)-330.5(Fig.)-335(2\).)]TJ
/F7 1 Tf
9.9626 0 0 9.9626 310.8472 596.636 Tm
[(SHED)-9(-CM)-335.3(stimul)-9.9(ates)-334.3(neur)-8.4(ite)-331.8(outgrow)-10.9(th)]TJ
0 -1.2007 TD
[(from)-334.1(and)-341.4(increa)-7.4(ses)-331.6(the)-339.2(viability)-339.4(of)-339.2(DRG)-338(neuro)-8.3(ns)]TJ
/F3 1 Tf
9.843 0 0 9.843 320.8251 566.8723 Tm
[(To)-262.6(determine)-274.1(the)-269.5(effects)-270.3(of)-267.1(SHED-CM)-271.6(on)-267.1(neuritogenesis,)]TJ
-1.0137 -1.1174 TD
[(a)-503.3(neurite)-505.6(outgrowth)-506.3(assay)-502.8(was)-506.2(performed.)-506.4(DRG)-501.4(neurons)]TJ
0 -1.1116 TD
[(were)-433.8(treated)-437.6(with)-431.8(SHED-CM)-438.6(or)-434.1(DMEM)-434.5(\()]TJ
/F6 1 Tf
17.8263 0 TD
(-)Tj
/F3 1 Tf
.5529 0 TD
[(\))-433(for)-429.5(48)-169.2(h)-433.1(and)]TJ
-18.3792 -1.1116 TD
[(subsequently)-418.6(immunostained)-416.7(with)-414.5(a)]TJ
/F8 1 Tf
15.3035 0 TD
(b)Tj
/F3 1 Tf
.5529 0 TD
[(-III-tubulin)-416.6(antibody)]TJ
-15.8565 -1.1174 TD
[(to)-474.6(measure)-473(neuritis.)-474.4(The)-478(average)-478.9(neurite)-471(outgrowth)-477.6(\()]TJ
/F8 1 Tf
22.6241 0 TD
(m)Tj
/F3 1 Tf
.5529 0 TD
(m\))Tj
-23.1771 -1.1116 TD
[(was)-293.1(signicantly)-292.2(greater)-293.5(in)-290.3(neurons)-290.9(treated)-293.6(with)-293.5(SHED-CM)]TJ
T*
[(than)-334(in)-330.6(those)-334.9(treated)-339.7(with)-333.8(DMEM)-336.6(\()]TJ
/F6 1 Tf
14.5836 0 TD
(-)Tj
/F3 1 Tf
.5529 0 TD
.3294 Tc
[(\)\()329.4(*)]TJ
/F9 1 Tf
1.4975 0 TD
0 Tc
(P)Tj
/F6 1 Tf
.7833 0 TD
(<)Tj
/F3 1 Tf
.7142 0 TD
[(0.05;)-336.3(Fig.)-335(3B\).)]TJ
-17.1178 -1.1116 TD
[(To)-331.7(compare)-334.9(the)-332.9(cell)-329.5(viabilities)-337.6(of)-330.5(DRG)-334.4(neurons)-337(cultured)]TJ
-1.0137 -1.1174 TD
[(with)-414.5(SHED-CM)-421.3(and)-419.2(DMEM)-417.2(\()]TJ
/F6 1 Tf
12.8211 0 TD
(-)Tj
/F3 1 Tf
.5529 0 TD
[(\),)-419.2(CCK8)-416.3(assays)-417.3(were)-422.3(per-)]TJ
-13.374 -1.1116 TD
[(formed.)-415.6(SHED-CM)-415.6(signicantly)-418.9(increased)-423.4(the)-413.5(viability)-420.7(of)]TJ
T*
[(DRG)-334.4(neurons)-337(relative)-335.2(to)-336.4(DMEM)-330.8(\()]TJ
/F6 1 Tf
14.1746 0 TD
(-)Tj
/F3 1 Tf
.5529 0 TD
.3351 Tc
[(\)\()335.1(*)]TJ
/F9 1 Tf
1.5033 0 TD
0 Tc
(P)Tj
/F6 1 Tf
.7776 0 TD
(<)Tj
/F3 1 Tf
.72 0 TD
[(0.05;)-336.3(Fig.)-329.2(3C\).)]TJ
/F7 1 Tf
9.9626 0 0 9.9626 310.8472 435.3448 Tm
[(SHED)-9(-CM)-335.3(stimul)-9.9(ates)-334.3(angio)-9.5(genesis)-341.1(in)-334(vitro)]TJ
/F3 1 Tf
9.843 0 0 9.843 320.8251 417.4865 Tm
[(T)15(o)-352.4(de)19.7(te)19.6(rm)19.6(in)20.7(e)-347.8(t)0(h)15(e)-347.8(ef)19.7(fe)19.7(ct)-337.6(of)-342(SH)20.1(ED)18.8(-C)19.8(M)-347.6(o)0(n)-342(a)0(n)19.7(g)0(i)20.7(o)0(g)20.8(e)0(n)19.7(e)0(s)19.9(i)0(s)15.1(,)-343.3(a)]TJ
-1.0137 -1.1116 TD
[(tu)20.7(be)-239.5(fo)15.1(rm)19.6(at)19.5(io)20.7(n)-248.8(a)0(s)19.9(s)0(a)14.1(y)-248.8(wa)18.8(s)-248.6(p)0(e)13.9(r)0(f)20.9(o)0(r)20.9(m)0(e)18.4(d)0(.)-240.7(H)0(U)19.1(V)0(E)18.8(C)0(s)-244.9(s)0(e)19.9(e)0(d)19.7(e)0(d)-239.5(i)0(n)-244.2(E)0(M)]TJ
T*
[(di)20.7(d)-254.5(n)0(o)20.8(t)-260.4(ex)19.7(hi)20.7(bi)20.7(t)-260.4(t)0(u)20.7(b)0(e)-245.3(f)0(o)15.1(r)0(m)19.6(a)0(t)19.5(i)0(o)20.7(n)0(.)-246.4(I)0(n)-249.8(c)0(o)19.7(n)0(t)20.7(r)0(a)19.7(s)0(t)20.9(,)-256.9(HU)19.1(VE)18.8(Cs)-250.7(ex)19.7(po)20.8(se)19.9(d)]TJ
T*
[(to)-267.3(SH)20.1(ED)18.8(-C)19.8(M,)-270.4(VE)18.8(GF)20.1(,)-274.1(o)0(r)-267.1(H)0(G)13.3(F)-277.4(ex)19.7(hi)20.7(bi)20.7(te)19.5(d)-277.6(n)0(o)20.8(t)0(i)20.6(c)0(e)12.8(a)0(b)19.7(l)0(e)-268.4(i)0(n)20.7(c)0(r)19.7(e)0(a)18.5(s)0(e)19.9(s)]TJ
0 -1.1174 TD
[(in)-486.1(le)19.5(ng)20.8(th)15(.)-493(Q)0(u)14.2(a)0(n)19.7(t)0(i)20.6()0(c)19.8(a)0(t)19.5(i)0(o)20.7(n)-502.2(of)-486(tu)20.7(be)-487.2(le)19.5(ng)15.1(th)-486.1(yi)20.7(el)19.5(de)19.7(d)-502.2(v)0(a)19.7(l)0(u)20.7(e)0(s)-487(o)0(f)]TJ
0 -1.1116 TD
[(3,)18.5(02)20.8(6)]TJ
/F6 1 Tf
2.3672 0 TD
()Tj
/F3 1 Tf
.7084 0 TD
[(69)20.8(0)-404.3(p)0(i)15(x)0(e)19.7(l)0(s)-393.8(w)0(i)14.1(t)0(h)-394(E)0(M)18.7(,)-406.6(9,)18.5(72)20.8(3)]TJ
/F6 1 Tf
11.3005 0 TD
()Tj
/F3 1 Tf
.7084 0 TD
[(97)-399.6(pi)20.7(xe)19.7(ls)-393.8(wi)14.1(th)-394(SH)20.1(ED)18.8(-)]TJ
-15.0847 -1.1116 TD
[(CM)18.9(,)-245.3(1)0(3)20.8(,)0(2)18.5(0)0(0)]TJ
/F6 1 Tf
4.8842 0 TD
()Tj
/F3 1 Tf
.7084 0 TD
[(38)20.8(6)-248.8(p)0(i)20.7(x)0(e)13.9(l)0(s)-232.5(w)0(i)14.1(t)0(h)-232.7(V)0(E)13(G)0(F)20.1(,)-245.3(an)19.7(d)-248.8(1)0(5)20.8(,)0(1)18.5(3)0(5)]TJ
/F6 1 Tf
13.9154 0 TD
()Tj
/F3 1 Tf
.7084 0 TD
[(97)20.8(2)-248.8(p)0(i)20.7(x)0(e)19.7(l)0(s)]TJ
-20.2166 -1.1174 TD
[(wi)19.8(th)-388.2(HG)19.1(F.)-384.5(Th)19.6(e)-399.7(r)0(e)19.7(s)0(p)21(e)0(c)18.5(t)0(i)20.6(v)0(e)-389.3(n)0(u)20.8(m)0(b)19.6(e)0(r)19.7(s)-398.3(of)-388.1(jo)20.7(in)20.7(ts)-388(we)18.8(re)-389.2(13)]TJ
/F6 1 Tf
22.1115 0 TD
()Tj
/F3 1 Tf
.7084 0 TD
[(5.)18.5(3,)]TJ
-22.82 -1.1116 TD
(42)Tj
/F6 1 Tf
1.1462 0 TD
()Tj
/F3 1 Tf
.7084 0 TD
[(4.)18.5(3,)-597.8(69)20.8(.3)]TJ
/F6 1 Tf
3.9569 0 TD
()Tj
/F3 1 Tf
.7084 0 TD
[(4.)18.5(2,)-597.8(an)19.7(d)-611.6(6)0(2)20.8(.)0(3)]TJ
/F6 1 Tf
5.9959 0 TD
()Tj
/F3 1 Tf
.7084 0 TD
[(4.)18.5(9.)-597.8(HU)19.1(VE)18.8(Cs)-602.1(cu)19.7(lt)20.6(ur)15.1(ed)-596.6(in)]TJ
-13.2243 -1.1116 TD
[(SH)20.1(ED)18.8(-C)19.8(M)-307.3(e)0(x)13.9(h)0(i)20.7(b)0(i)20.7(t)0(e)19.5(d)-306.4(si)15.1(gn)20.8(i)20.9(ca)18.5(nt)-301.8(in)20.7(cr)19.7(ea)18.5(se)19.9(s)-311.9(i)0(n)-296.1(t)0(u)20.7(b)0(e)-302.9(l)0(e)19.5(n)0(g)20.8(t)0(h)-296.1(a)0(n)19.7(d)]TJ
0 -1.1174 TD
[(jo)20.7(in)20.7(t)-323.8(n)0(u)20.8(m)0(b)19.6(e)0(r)-314.4(\()0(*)]TJ
/F9 1 Tf
6.2781 0 TD
(P)Tj
/F6 1 Tf
.766 0 TD
(<)Tj
/F3 1 Tf
.7142 0 TD
[(0.)24.3(05)20.8(;)-323.8(F)0(i)15.1(g)0(.)-309.8(4)0(B)19.8(,)-326(C\))19.8(.)]TJ
ET
q
299.439 0 0 133.739 155.168 143.32 cm
/Im1 Do
Q
BT
/F10 1 Tf
9.845 0 0 9.845 59.7543 126.5385 Tm
[(FIG.)-336.7(1.)]TJ
/F3 1 Tf
4.1174 0 TD
[(Effects)-338.7(of)-336(SHED-CM)-339.6(on)-336(SC)-336.8(migration)-340(and)-338.1(proliferation.)]TJ
/F10 1 Tf
23.6964 0 TD
(\(A\))Tj
/F3 1 Tf
1.7218 0 TD
[(Transwell)-341.7(migration)-334.2(assay.)-338.6(The)-339.3(migration)-340(of)-336(SCs)]TJ
-29.5356 -1.0077 TD
[(cultured)-258.2(in)-261.3(SHED-CM)-259(was)-258.2(enhanced)-254.5(relative)-265.3(to)-255.5(those)-253.7(cultured)-258.2(in)-255.5(DMEM)-261(\()]TJ
/F6 1 Tf
30.1517 0 TD
(-)Tj
/F3 1 Tf
.5528 0 TD
[(\).)-252(DMEM)-261(\()]TJ
/F6 1 Tf
4.532 0 TD
(-)Tj
/F3 1 Tf
.5528 0 TD
[(\),)-252(SHED-CM,)-262.4(DMEM)-255.2(\(15%)-258.3(FBS\):)]TJ
/F9 1 Tf
-35.7894 -1.0135 TD
(n)Tj
/F6 1 Tf
.668 0 TD
(=)Tj
/F3 1 Tf
.7198 0 TD
[(5)-340.7(per)-343.9(group.)-336.6(Data)-347.1(are)-339.3(shown)-345.4(as)-342.7(mean)]TJ
/F6 1 Tf
15.6575 0 TD
()Tj
/F3 1 Tf
.7141 0 TD
[(SD.)-345.8(*)]TJ
/F9 1 Tf
2.361 0 TD
(P)Tj
/F6 1 Tf
.7832 0 TD
(<)Tj
/F3 1 Tf
.7198 0 TD
(0.05.)Tj
/F10 1 Tf
2.338 0 TD
(\(B\))Tj
/F3 1 Tf
1.6757 0 TD
[(Cell)-340.5(proliferation)-349.9(assay.)-338.6(SC)-342.6(proliferation)-344.1(is)-341.7(reported)-344.4(as)-342.7(the)]TJ
-25.637 -1.0135 TD
[(absorbance)-290.8(at)-285.5(450)-164.2(nm)-285.3(following)-286.7(MTT)-288.3(analysis.)-284.3(The)-287.5(proliferation)-286.6(of)-284.1(SCs)-285.8(cultured)-287(in)-284.3(SHED-CM)-287.8(was)-287(also)-287.3(enhanced)-289(relative)]TJ
T*
[(to)-295.8(those)-299.8(cultured)-292.8(in)-295.8(DMEM)-295.5(\()]TJ
/F6 1 Tf
11.8799 0 TD
(-)Tj
/F3 1 Tf
.5528 0 TD
[(\).)-298.1(Cells)-295.2(cultured)-298.5(in)-295.8(DMEM)-295.5(\(15%)-292.9(FBS\))-298.3(were)-295.1(used)-298.6(as)-296.7(a)-295.8(positive)-291.6(control.)-299.7(DMEM)-295.5(\()]TJ
/F6 1 Tf
32.9907 0 TD
(-)Tj
/F3 1 Tf
.5528 0 TD
[(\),)-292.3(SHED-)]TJ
-45.9762 -1.0077 TD
[(CM,)-485.1(DMEM)-479.8(\(15%)-482.9(FBS\):)]TJ
/F9 1 Tf
11.3789 0 TD
(n)Tj
/F6 1 Tf
.668 0 TD
(=)Tj
/F3 1 Tf
.7198 0 TD
[(5)-479(per)-482.1(group.)-480.5(Data)-485.3(are)-483.3(shown)-477.8(as)-480.9(mean)]TJ
/F6 1 Tf
16.6307 0 TD
()Tj
/F3 1 Tf
.7198 0 TD
[(SD.)-484(*)]TJ
/F9 1 Tf
2.4992 0 TD
(P)Tj
/F6 1 Tf
.7832 0 TD
(<)Tj
/F3 1 Tf
.7198 0 TD
[(0.05.)-481.9(SHED,)-482.2(stem)-478.5(cells)-485.4(from)-477.3(human)]TJ
-34.1194 -1.0135 TD
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