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To increase the reliability of green fluorescent protein (GFP) use
http://hdl.handle.net/2237/24974
http://hdl.handle.net/2237/249746fe129fe-7751-43b9-8bb0-909ece0058b3
名前 / ファイル | ライセンス | アクション |
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nuresearch_e001.pdf (6.2 MB)
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Item type | その他 / Others(1) | |||||
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公開日 | 2016-10-20 | |||||
タイトル | ||||||
タイトル | To increase the reliability of green fluorescent protein (GFP) use | |||||
言語 | en | |||||
アクセス権 | ||||||
アクセス権 | open access | |||||
アクセス権URI | http://purl.org/coar/access_right/c_abf2 | |||||
権利 | ||||||
言語 | en | |||||
権利情報 | (c) Academic Research & Industry-Academia-Government Collaboration,Nagoya University | |||||
抄録 | ||||||
内容記述 | Green fluorescent protein (GFP) is one of the most important tools used in contemporary bioscience. Dr. Shoji Segami, Prof. Masayoshi Maeshima and his research group at the Graduate School of Bioagricultural Sciences, Nagoya University have found that when GFPs are genetically inserted into the proton pump in the vacuole membranes of plants, GFP-dimers artificially stuck to each membrane, resulting in odd structures in the vacuole known as "bulbs." Furthermore, by inserting monomeric GFP, which does not dimerize, the formation of these odd structures was completely inhibited. Thus, the vacuoles in living cells can dynamically change their shapes and form the artifact of bulbs when influenced by dimeric GFPs. Actual morphology of vacuole can be observed by visualization of the proton pump with monomeric GFP. The result has been published in The Plant Cell (vol. 26: 3416-3434, August 2014). | |||||
言語 | en | |||||
内容記述タイプ | Abstract | |||||
内容記述 | ||||||
内容記述 | Japanese version: http://hdl.handle.net/2237/23625 | |||||
言語 | en | |||||
内容記述タイプ | Other | |||||
出版者 | ||||||
言語 | en | |||||
出版者 | Academic Research & Industry-Academia-Government Collaboration,Nagoya University | |||||
言語 | ||||||
言語 | eng | |||||
資源タイプ | ||||||
資源 | http://purl.org/coar/resource_type/c_1843 | |||||
タイプ | other | |||||
関連情報 | ||||||
関連タイプ | isVersionOf | |||||
識別子タイプ | URI | |||||
関連識別子 | http://www.aip.nagoya-u.ac.jp/en/public/nu_research/highlights/detail/0001876.html | |||||
書誌情報 |
en : NU Research 巻 E-001, 発行日 2015-01-29 |
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著者版フラグ | ||||||
値 | publisher | |||||
URI | ||||||
識別子 | http://www.aip.nagoya-u.ac.jp/en/public/nu_research/highlights/detail/0001876.html | |||||
識別子タイプ | URI | |||||
URI | ||||||
識別子 | http://hdl.handle.net/2237/24974 | |||||
識別子タイプ | HDL |