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PRDM14 and BLIMP1 control the development of chicken primordial germ cells
http://hdl.handle.net/2237/00031185
http://hdl.handle.net/2237/00031185576395e5-f4f3-43d8-a749-97dd8e534a4e
名前 / ファイル | ライセンス | アクション |
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Okuzaki et al-DevBiol.revise2nd (7.2 MB)
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Item type | 学術雑誌論文 / Journal Article(1) | |||||
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公開日 | 2020-01-15 | |||||
タイトル | ||||||
タイトル | PRDM14 and BLIMP1 control the development of chicken primordial germ cells | |||||
言語 | en | |||||
著者 |
Okuzaki, Yuya
× Okuzaki, Yuya× Kaneoka, Hidenori× Suzuki, Takayuki× Hagihara, Yota× Nakayama, Yuki× Murakami, Seitaro× Murase, Yusuke× Kuroiwa, Atsushi× Iijima, Shinji× Nishijima, Ken-ichi |
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アクセス権 | ||||||
アクセス権 | open access | |||||
アクセス権URI | http://purl.org/coar/access_right/c_abf2 | |||||
権利 | ||||||
言語 | en | |||||
権利情報 | © 2019. This manuscript version is made available under the CC-BY-NC-ND 4.0 license http://creativecommons.org/licenses/by-nc-nd/4.0/ | |||||
キーワード | ||||||
主題Scheme | Other | |||||
主題 | Chicken | |||||
キーワード | ||||||
主題Scheme | Other | |||||
主題 | Primordial germ cells | |||||
キーワード | ||||||
主題Scheme | Other | |||||
主題 | PRDM14 | |||||
キーワード | ||||||
主題Scheme | Other | |||||
主題 | BLIMP1 | |||||
キーワード | ||||||
主題Scheme | Other | |||||
主題 | CVH | |||||
抄録 | ||||||
内容記述 | The differentiation of primordial germ cells (PGCs) is a fundamental step in development. PR domain-containing protein 14 (PRDM14) and B lymphocyte-induced maturation protein 1 (BLIMP1) play pivotal roles in mouse PGC specification. In the present study, we assessed the roles of chicken orthologs of PRDM14 and BLIMP1 in PGC development. PRDM14 and BLIMP1 were expressed in blastodermal cells and PGCs. The in vivo knockdown of PRDM14 or BLIMP1 by introducing a replication-competent retroviral vector expressing shRNAs to the blastodermal stage of embryos reduced the number of SSEA-1 or chicken vasa homologue-positive PGCs on day 5.5–6.5. Since the inhibition of Activin receptor-like kinase 4/5/7 in cultured PGCs reduced the expression of PRDM14, BLIMP1, and NANOG, and that of MEK inhibited PRDM14 expression, the expression of these genes seems to be controlled by Activin A and FGF2 signaling. Overall, PRDM14, BLIMP1, and NANOG seem to be involved in the self-renewal of PGCs in cultured PGCs and embryos. | |||||
言語 | en | |||||
内容記述タイプ | Abstract | |||||
内容記述 | ||||||
内容記述 | ファイル公開:2020-11-01 | |||||
言語 | ja | |||||
内容記述タイプ | Other | |||||
出版者 | ||||||
言語 | en | |||||
出版者 | Elsevier | |||||
言語 | ||||||
言語 | eng | |||||
資源タイプ | ||||||
資源タイプresource | http://purl.org/coar/resource_type/c_6501 | |||||
タイプ | journal article | |||||
出版タイプ | ||||||
出版タイプ | AM | |||||
出版タイプResource | http://purl.org/coar/version/c_ab4af688f83e57aa | |||||
DOI | ||||||
関連タイプ | isVersionOf | |||||
識別子タイプ | DOI | |||||
関連識別子 | https://doi.org/10.1016/j.ydbio.2019.06.018 | |||||
ISSN(print) | ||||||
収録物識別子タイプ | PISSN | |||||
収録物識別子 | 0012-1606 | |||||
書誌情報 |
en : Developmental Biology 巻 455, 号 1, p. 32-41, 発行日 2019-11-01 |
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著者版フラグ | ||||||
値 | author |