2024-03-28T10:35:02Z
https://nagoya.repo.nii.ac.jp/oai
oai:nagoya.repo.nii.ac.jp:00003913
2023-11-16T06:58:41Z
499:508:509:510
Effects of sodium hyaluronate on experimental osteoarthritis in rabbit knee joints
Han, Fei
Ishiguro, Naoki
Ito, Takayasu
Sakai, Tadahiro
Iwata, Hisashi
open access
anterior cruciate ligament (ACL)
osteoarthritis
matrix metalloproteinase (MMP)
Tissue inhibitor of metalloproteinase (TIMP)
proteoglycan
The aim of this study was to examine the effects of intraarticular administration of hyaluronan (HA) on cartilage degradation. Using a partial menisectomy model of osteoarthritis (OA) in the rabbit knee, the authors investigated the catabolic and anabolic changes induced by intraarticular injection of HA. To analyze anabolic changes, the authors assessed cell proliferation by measuring [3H] thymidine uptake, and proteoglycan biosynthesis by noting [35S] sulfate incorporation. For catabolic changes, messenger ribonucleic acid (mRNA) expression of interstitial collagenase (MMP-1), stromelysin-1 (MMP-3), and tissue inhibitor of metalloproteinase-1 (TIMP-1) in cartilage and synovium were detected with reverse transcriptase polymerase chain reaction (RT-PCR). Of significance for blocking the development of early OA in chondrocytes was the finding that total proteoglycan synthesis in the HA treatment group was significantly higher than in the controls. At the mRNA level in cartilage and synovium, HA inhibited MMP-3 and TIMP-1 production in the same way in the HA treatment group, while not affecting MMP-1 production. Thus it can be concluded that HA affects cartilage catabolism and anabolism to prevent the progress of OA.
Nagoya University School of Medicine
1999-11
eng
departmental bulletin paper
VoR
https://doi.org/10.18999/nagjms.62.3-4.115
http://hdl.handle.net/2237/5343
https://nagoya.repo.nii.ac.jp/records/3913
10.18999/nagjms.62.3-4.115
2186-3326
0027-7622
Nagoya Journal of Medical Science
62
3-4
115
126
https://nagoya.repo.nii.ac.jp/record/3913/files/nj6235.pdf
application/pdf
137.7 kB
2018-02-19