2024-03-28T10:17:05Z
https://nagoya.repo.nii.ac.jp/oai
oai:nagoya.repo.nii.ac.jp:00003944
2023-11-16T06:42:25Z
499:508:509:515
Effect of lactase preparations in asymptomatic individuals with lactase deficiency : gastric digestion of lactose and breath hydrogen analysis
Gao, Kai-Ping
Mitsui, Takahiro
Fujiki, Kotoyo
Ishiguro, Hiroshi
Kondo, Takaharu
open access
lactase deficiency
lactose intolerance
galactose
lactase
breath hydrogen
We compared two lactase preparations derived from Aspergillus orizae (AOL) and Penicillinase multicolor (PML) for stability in the stomach and overall enzymatic activity in 10 asymptomatic subjects with lactase deficiency. The subjects were given 10,000 FCC units of either AOL or PML 30 min prior to or simultaneously with 300 ml of milk. Gastric juice was withdrawn through a nasogastric tube immediately after and every 15 min for 60 min, and breath was sampled before and every 15 min for 6 h after the milk ingestion. When lactase was given simultaneously with the milk, gastric juice lactase activity and galactose concentration were significantly higher than the control levels. When lactase preparations were given 30 min prior to the milk, neither lactase activity nor galactose was detected in the gastric juice. The pH of the gastric juice was about 6.0 after the milk ingestion. Breath hydrogen did not increase when milk was ingested simultaneously with enzymes, but did increase if enzymes were given 30 min prior to milk ingestion. There were no significant differences in lactase activity, galactose concentration in gastric juice, or breath hydrogen when AOL and PML were compared. In conclusion, with exogenous lactase, digestion of lactose begins in the stomach when pH is raised to 6.0 by the buffering action of milk. Lactase preparations are effective assessed by breath hydrogen analysis in asymptomatic individuals with lactase deficiency if the enzymes are given simultaneously with milk.
Nagoya University School of Medicine
2002-05
eng
departmental bulletin paper
VoR
https://doi.org/10.18999/nagjms.65.1-2.21
http://hdl.handle.net/2237/5376
https://nagoya.repo.nii.ac.jp/records/3944
10.18999/nagjms.65.1-2.21
2186-3326
0027-7622
Nagoya Journal of Medical Science
65
1-2
21
28
https://nagoya.repo.nii.ac.jp/record/3944/files/nj6513.pdf
application/pdf
57.2 kB
2018-02-19