2024-03-28T16:44:10Z
https://nagoya.repo.nii.ac.jp/oai
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2023-01-16T04:59:11Z
336:695:696
Identification and characterization of Drosophila homolog of Rho-kinase
Mizuno, Tomoaki
Amano, Mutsuki
Kaibuchi, Kozo
Nishida, Yasuyoshi
open access
Rho effector
actin cytoskeleton
morphogenesis
low stringency PCR
two-hybrid analysis
in vitro kinase assay
The Rho family of small GTPases and their associated regulators and targets are essential mediators of diverse morphogenetic events in development. Mammalian Rho‐kinase/ROKα, one of the targets of Rho, has been shown to bind to Rho in GTP-bound form and to phosphorylate the myosin light chain (MLC) and the myosin binding subunit (MBS) of myosin phosphatase, resulting in the activation of myosin. Thus, Rho‐kinase/ROKα has been suggested to play essential roles in the formation of stress fibers and focal adhesions. We have identified the Drosophila homolog of Rho‐kinase/ROKα, DRho‐kinase, which has conserved the basic structural feature of Rho‐kinase/ROKα consisting of the N‐terminal kinase, central coiled-coil and C‐terminal pleckstrin homology (PH) domains. A two‐hybrid analysis demonstrated that DRho‐kinase interacts with the GTP‐bound form of the Drosophila Rho, Drho1, at the conserved Rho-binding site. DRho‐kinase can phosphorylate MLC and MBS, preferable substrates for bovine Rho‐kinase, in vitro. DRho‐kinase is ubiquitously expressed throughout development, in a pattern essentially identical to that of Drho1. These results suggest that DRho‐kinase is an effector of Drho1.
Elsevier
1999-10-01
eng
journal article
VoR
http://hdl.handle.net/2237/7297
https://nagoya.repo.nii.ac.jp/records/5691
https://doi.org/10.1016/S0378-1119(99)00351-0
0378-1119
Gene
238
2
437
444
https://nagoya.repo.nii.ac.jp/record/5691/files/DRho_kinase_final.pdf
application/pdf
417.5 kB
2018-02-19