2024-03-28T13:12:01Z
https://nagoya.repo.nii.ac.jp/oai
oai:nagoya.repo.nii.ac.jp:00005841
2023-01-16T04:54:30Z
499:500:501
Macrophages in Muscle Layer of Gastrointestinal Tract : Impairment of Muscle Contraction by Treatment with Lipopolysaccharide
Torihashi, Shigeko
鳥橋, 茂子
open access
Copyright (c) 2001 By the Japan Society of Histochemistry and Cytochemistry
Macrophage
Gastrointestinal tract
Smooth muscle
Inducible nitric oxide synthase
Cyclooxygenase-2
The morphology and function of resident macrophages in the muscle layer and in the subserosa of the gastrointestinal tract are described. FITC-dextran injected via the tail vein of a mouse or rat demonstrated a regular arrangement of macrophages on a whole mount preparation of the muscle layer. Electron microscopy revealed inactive phagocytosis under normal conditions. After a 4-8 hr incubation of rat ileal muscle with lipopolysaccharide (LPS) to investigate its effect on muscle contraction, macrophages began to express inducible nitric oxide synthase (iNOS) and released nitric oxide. This macrophage response was accompanied by the expression of cyclooxygenase-2 (COX-2) immunoreactivity and the production of prostaglandins. The muscarinic agonist-induced contraction was greatly inhibited by LPS treatment, and this inhibition was reversed by either iNOS or COX-2 inhibitors. We concluded that one of the functions of muscular macrophages was the modulation of smooth muscle contraction under pathological conditions. The mechanism of iNOS upregulation indicated by our experiment is discussed.
Japan Society of Histochemistry and Cytochemistry
2001
eng
journal article
VoR
http://hdl.handle.net/2237/7457
https://nagoya.repo.nii.ac.jp/records/5841
https://doi.org/10.1267/ahc.34.219
0044-5991
Acta Histochem. Cytochem
34
4
219
222
https://nagoya.repo.nii.ac.jp/record/5841/files/acta_his_cyt_34_219.pdf
application/pdf
151.4 kB
2018-02-19