2024-03-28T13:15:54Z
https://nagoya.repo.nii.ac.jp/oai
oai:nagoya.repo.nii.ac.jp:00007682
2023-01-16T03:46:56Z
336:695:696
Mi-2 chromatin remodeling factor functions in sensory organ development through proneural gene repression in Drosophila
YAMASAKI, Yasutoyo
NISHIDA, Yasuyoshi
open access
The definitive version is available at www.blackwell-synergy.com
NuRD complex
chromatin remodeling factor
sensory organ development
proneural gene
macrochaete
Mi-2, the central component of the nucleosome remodeling and histone deacetylation (NuRD) complex, is known as an SNF2-type ATP-dependent nucleosome remodeling factor. No morphological mutant phenotype of Drosophila Mi-2 (dMi-2) had been reported previously, however, we found that rare escapers develop into adult flies showing an extra bristle phenotype. The dMi-2 enhanced the phenotype of ac^Hw49c, which is a dominant gain-of-function allele of achaete (ac) and produces extra bristles. Consistent with these observations, the ac-expressing proneural clusters were expanded, and extra sensory organ precursors (SOPs) were formed in the dMi-2 mutant wing discs. Immunostaining of polytene chromosome showed that dMi-2 binds to the ac locus, and dMi-2 and acetylated hisotones distribute on polytene chromosomes in a mutually exclusive manner. The chromatin immunoprecipitation assay of the wing imaginal disc also demonstrated a binding of dMi-2 on the ac locus. These results suggest that the Drosophila Mi-2/NuRD complex functions in neuronal differentiation through the repression of proneural gene expression by chromatin remodeling and histone deacetylation.
Blackwell
2006
eng
journal article
AM
http://hdl.handle.net/2237/9393
https://nagoya.repo.nii.ac.jp/records/7682
https://doi.org/10.1111/j.1440-169X.2006.00880.x
Development, Growth & Differentiation
48
7
411
418
https://nagoya.repo.nii.ac.jp/record/7682/files/DGD-dMi-2.pdf
application/pdf
2.9 MB
2018-02-19