2024-03-28T18:27:51Z
https://nagoya.repo.nii.ac.jp/oai
oai:nagoya.repo.nii.ac.jp:00012571
2023-01-16T04:59:25Z
336:695:696
Reconstruction of Motile Actin Networks in Giant Liposome
Takiguchi, Kingo
Negishi, Makiko
Tanaka-Takiguchi, Yohko
Homma, Michio
Yoshikawa, Kenichi
open access
© 2011 IEEE. Personal use of this material is permitted. Permission from IEEE must be obtained for all other uses, in any current or future media, including reprinting/republishing this material for advertising or promotional purposes, creating new collective works, for resale or redistribution to servers or lists, or reuse of any copyrighted component of this work in other works.
To construct a simple model cellular system exhibiting the property of self-propelled motion, cell-sized giant liposomes encapsulating desired amounts of actoHMM, a mixture of actin filament (F-actin) and heavy meromyosin (HMM, an actin-related molecular motor), have been prepared. We adapted the methodology of spontaneous transfer of a water droplet through oil/water interface in the presence of phospholipid and successful obtained stable giant liposome with the inner physiological biopolymer solution. We introduced ATP to the bathing solution of liposome encapsulating actoHMM, in which bilayer membrane α-hemolysin, a bacterial membrane pore-forming toxin, is embedded. In this system, ATP is supplied into the inner volume of liposome through the protein pores in a passive manner. Accompanied by the ATP supply, actin networks or bundles that have encapsulated in the liposomes exhibited specific morphological change, being attributable to the active sliding between F-actin and HMM. Remarkable difference in the behavior of F-actins is found; i.e., inside the liposome, almost all the F-actins situate around the inner periphery of the liposome, whereas, in the bulk solution, actin bundles form an aster-like structure.
IEEE
2010
eng
journal article
AM
http://hdl.handle.net/2237/14455
https://nagoya.repo.nii.ac.jp/records/12571
https://doi.org/10.1109/MHS.2010.5669559
978-1-4244-7995-5
International Symposium on Micro-NanoMechatronics and Human Science (MHS)
150
155
https://nagoya.repo.nii.ac.jp/record/12571/files/1117.pdf
application/pdf
1.7 MB
2018-02-20