2024-03-28T13:27:45Z
https://nagoya.repo.nii.ac.jp/oai
oai:nagoya.repo.nii.ac.jp:00013020
2023-11-16T06:36:34Z
499:508:509:1326
IDENTIFICATION OF A STREPTOCOCCUS PYOGENES SF370 GENE INVOLVED IN PRODUCTION OF C-DI-AMP
KAMEGAYA, TAICHI
KURODA, KENJI
HAYAKAWA, YOSHIHIRO
open access
Streptococcus pyogenes
c-di-AMP
Diadenylate cyclase
spyDAC
Here we show that bis(3’–5’) cyclic diadenylic acid (c-di-AMP) and a diadenylate cyclase (DAC) domain protein involved in the biosynthesis of c-di-AMP were identified in Streptococcus pyogenes. The matrix-assisted laser desorption ionization (MALDI)-time of flight (TOF) mass spectrum of the cell extract of S. pyogenes, which showed a fragment pattern very similar to that of the authentic sample of c-di-AMP, revealed that S. pyogenes produces c-di-AMP in the cell. Subsequently, we confirmed by an in vitro experiment that the production of c-di-AMP in the cell is due to the action of Spy1036 gene encoding a DAC domain protein named spyDAC, which is a new protein different from a well-known diadenylate cyclase. Moreover, the experiment gave a product with a molecular weight of 657.021, which is consistent with the molecular weight of c-di-AMP. Furthermore, the mass spectral fragment pattern of the product obtained by the in vitro biosynthesis is quite similar to that of the product produced by the above in vivo experiment. This in vitro production of c-di-AMP indicated that spyDAC in S. pyogenes actually catalyzes the in vivo biosynthesis of c-di-AMP from ATP.
名古屋大学博士学位論文 学位の種類 : 博士(医学)(課程) 学位授与年月日:平成23年3月25日 亀ヶ谷太一氏の博士論文として提出された
Nagoya University School of Medicine
2011-02
eng
departmental bulletin paper
VoR
https://doi.org/10.18999/nagjms.73.1-2.49
http://hdl.handle.net/2237/14915
https://nagoya.repo.nii.ac.jp/records/13020
10.18999/nagjms.73.1-2.49
http://www.med.nagoya-u.ac.jp/medlib/nagoya_j_med_sci/7312/7312.html
2186-3326
0027-7622
Nagoya Journal of Medical Science
73
1-2
49
57
https://nagoya.repo.nii.ac.jp/record/13020/files/v73n12p49_57.pdf
application/pdf
189.7 kB
2018-02-20