2024-03-29T01:42:02Z
https://nagoya.repo.nii.ac.jp/oai
oai:nagoya.repo.nii.ac.jp:00014756
2023-01-16T04:37:29Z
499:960:1165
Alteration of N-linked oligosaccharide structures of human chorionic gonadotropin β -subunit by disruption of disulfide bonds
MORIWAKI, TAKAYUKI
SUGANUMA, NOBUHIKO
FURUHASHI, MADOKA
KIKKAWA, FUMITAKA
TOMODA, YUTAKA
BOIME, IRVING
NAKATA, MUNEHIRO
MIZUOCHI, TSUGUO
open access
The final publication is available at www.springerlink.com
human chorionic gonadotropin
hCG β-subunit
disulfide bond
N-linked oligosaccharide processing
The human chorionic gonadotropin β-subunit (hCGβ) is a glycoprotein in which 12 cysteine residues pair to form six intramolecular disulfide bonds. In order to elucidate the effect of each disulfide bond on glycosylation of the molecule, we analysed structures of asparagine-linked oligosaccharides of various recombinant hCGβ produced in Chinese hamster ovary (CHO) cells: wild-type hCGβ (βWT) and mutants in which any one of the six intramolecular disulfide bonds had been disrupted by site-directed mutagenesis. SDS-PAGE analysis of βWT and these mutants before and after digestion with endoglycosidase F and H revealed structural changes in the oligosaccharide moieties of some mutants. In addition, structural analysis of oligosaccharides obtained from metabolically labeled βWT and a mutant showed that the mutant contained additional high mannose type oligosaccharides. These results suggest that elimination of a specific disulfide bond, resulting in a change in the protein conformation, disturbs the normal assembly of the mature complex type oligosaccharides in the hCGβ molecule. Abbreviations: hCGβ, human chorionic gonadotropin β-subunit; βWT, wild type hCGβ; CHO, Chinese hamster ovary; Endo-H, endoglycosidase H; Endo-F, endoglycosidase F
名古屋大学博士学位論文 学位の種類 : 博士(医学)(論文) 学位授与年月日:平成9年2月28日 森脇崇之氏の博士論文として提出された
Springer
1997-02
eng
doctoral thesis
http://hdl.handle.net/2237/16683
https://nagoya.repo.nii.ac.jp/records/14756
https://doi.org/10.1023/A:1018593805890
0282-0080
Glycoconjugate Journal
14
2
225
229
乙第5164号
博士(医学)
1997-02-01
13901
名古屋大学
NAGOYA University
https://nagoya.repo.nii.ac.jp/record/14756/files/o5164.pdf
application/pdf
1.7 MB
2018-02-20