2024-03-29T06:51:29Z
https://nagoya.repo.nii.ac.jp/oai
oai:nagoya.repo.nii.ac.jp:00015126
2023-01-16T04:22:37Z
499:960:1165
Epidermal Hyperplasia and Appendage Abnormalities in Mice Lacking CD109
Mii, Shinji
Murakumo, Yoshiki
Asai, Naoya
Jijiwa, Mayumi
Hagiwara, Sumitaka
Kato, Takuya
Asai, Masato
Enomoto, Atsushi
Ushida, Kaori
Sobue, Sayaka
Ichihara, Masatoshi
Takahashi, Masahide
三井, 伸二
open access
NOTICE: This is the author's version of a work that was accepted for publication in The American Journal of Pathology. Changes resulting from the publishing process, such as peer review, editing, corrections, structural formatting, and other quality control mechanisms, may not be reflected in this document. Changes may have been made to this work since it was submitted for publication. A definitive version was subsequently published in The American Journal of Pathology. v.181, n.4, 2012, p.1180–1189, DOI: http://dx.doi.org/10.1016/j.ajpath.2012.06.021
CD109, a glycosylphosphatidylinositol-anchored glycoprotein, is highly expressed in several types of human cancer tissues, in particular, squamous cell carcinomas. In normal human tissues, human CD109 expression is limited to certain cell types including myoepithelial cells of the mammary, lacrimal, salivary, and bronchial glands and basal cells of the prostate and bronchial epithelium. Although CD109 has been reported to negatively regulate transforming growth factor-β signaling in keratinocytes in vitro, its physiologic role in vivo remains largely unknown. To investigate the function of CD109 in vivo, we generated CD109-deficient (CD109−/−) mice. Although CD109−/− mice were born normally, transient impairment of hair growth was observed. At histologic analysis, kinked hair shafts, ectatic hair follicles with an accumulation of sebum, and persistent hyperplasia of the epidermis and sebaceous glands were observed in CD109−/− mice. Immunohistochemical analysis revealed thickening of the basal and suprabasal layers in the epidermis of CD109−/− mice, which is where endogenous CD109 is expressed in wild-type mice. Although CD109 was reported to negatively regulate transforming growth factor-β signaling, no significant difference in levels of Smad2 phosphorylation was observed in the epidermis between wild-type and CD109−/− mice. Instead, Stat3 phosphorylation levels were significantly elevated in the epidermis of CD109−/− mice compared with wild-type mice. These results suggest that CD109 regulates differentiation of keratinocytes via a signaling pathway involving Stat3.
名古屋大学博士学位論文 学位の種類 : 博士(医学)(課程) 学位授与年月日:平成24年7月31日 三井伸二氏の博士論文として提出された
Elsevier
2012-10
eng
doctoral thesis
http://hdl.handle.net/2237/17140
https://nagoya.repo.nii.ac.jp/records/15126
https://doi.org/10.1016/j.ajpath.2012.06.021
0002-9440
The American Journal of Pathology
181
4
1180
1189
甲第9808号
博士(医学)
2012-10-01
13901
名古屋大学
Nagoya University
https://nagoya.repo.nii.ac.jp/record/15126/files/k9808.pdf
application/pdf
29.5 MB
2018-02-20