2024-03-28T11:01:46Z
https://nagoya.repo.nii.ac.jp/oai
oai:nagoya.repo.nii.ac.jp:00015492
2023-11-16T05:50:45Z
499:508:509:1463
Receptor Binding of Epidermal Growth Factor in Cultured Human Choriocarcinoma Cell Lines: Effects of Actinomycin-D and Methotrexate
CHEN, FAN
GOTO, SETSUKO
NAWA, AKIHIRO
OKAMOTO, TOMOMITSU
TOMODA, YUTAKA
open access
Epidermal Growth Factor
Receptor Binding
Choriocarcinoma
Actinomycin-D
Methotrexate
Binding of epidermal growth factor (EGF) to its receptor was evaluated in the four cultured choriocarcinoma cell lines BeWo, NaUCC-1, NaUCC-2. and NaUCC-3. Also, the effect of the anti-tumor drugs actinomycin-D (Act-D) and methotrexate (MTX) on the EGF receptor binding was investigated in these cell lines. Incubation of these cells with [125I]EGF at 37°C resulted in a higher binding than that at 22°C or at 4°C. These bindings were saturable during 30- to 60-min incubation, and were specific and reversible. Scatchard analysis showed that the maximal number of receptor binding sites was 2.89 x 10^3/cell in BeWo cells, 2.04 x 10^3/cell in NaUCC-1 cells, 1.84 x 10^3/cell in NaUCC-2 cells, and 1.01 x 10^3/cell in NaUCC-3 cells. Preincubation with Act-D or MTX for 24 hr decreased the number of receptor binding sites (26 %-53 %) and slightly increased the receptor binding affinities. Combination of the two drugs resulted in a further diminution of EGF receptor binding sites in BeWo, NaUCC-1, and NaUCC-3 cells, respectively, but reversed the Act-D effect in NaUCC-2 cells. These results indicated that choriocarcinoma tissue is rich in EGF receptors, that the anti-tumor drugs Act-D and MTX diminish the receptor binding sites in the tissue. and suggest that MTX might induce a drug resistance to Act-D in some choriocarcinoma tissue.
Nagoya University School of Medicine
1990-03
eng
departmental bulletin paper
VoR
https://doi.org/10.18999/nagjms.52.1-4.5
http://hdl.handle.net/2237/17510
https://nagoya.repo.nii.ac.jp/records/15492
10.18999/nagjms.52.1-4.5
http://www.med.nagoya-u.ac.jp/medlib/nagoya_j_med_sci/5214/5214.html
2186-3326
0027-7622
Nagoya Journal of Medical Science
52
1-4
5
11
https://nagoya.repo.nii.ac.jp/record/15492/files/v52n14p5_11.pdf
application/pdf
452.4 kB
2018-02-20