2024-03-29T09:57:32Z
https://nagoya.repo.nii.ac.jp/oai
oai:nagoya.repo.nii.ac.jp:00016458
2023-11-16T05:38:49Z
499:508:509:1504
SIMPLE AND RAPID ASSAY METHOD FOR SIMULTANEOUS QUANTIFICATION OF URINARY NICOTINE AND COTININE USING MICRO-EXTRACTION BY PACKED SORBENT AND GAS CHROMATOGRAPHY-MASS SPECTROMETRY
Iwai, Masae
Ogawa, Tadashi
Hattori, Hideki
Zaitsu, Kei
Ishii, Akira
Suzuki, Osamu
Seno, Hiroshi
open access
micro-extraction by packed sorbent (MEPS)
gas chromatography-mass spectrometry (GC-MS)
nicotine
cotinine
A simple and rapid method for determination of nicotine and cotinine levels in urine was developed using samples prepared by micro-extraction by packed sorbent (MEPS) and subjected to gas chromatography- mass spectrometry (GC-MS) analysis. This method provided good reproducibility, as well as good linearity of calibration curves in the range of 1–100 and 50–1000 ng/mL for quality control samples spiked with nicotine and cotinine, respectively. The detection limit of nicotine and cotinine was as low as 0.25 and 20 ng/mL, respectively. An evaporation procedure is not suitable for nicotine determination, thus an advantage of the present MEPS assay method is direct testing with GC-MS without the need for evaporation to a dry solvent. Our findings show that it may be useful for determining nicotine levels in various types of research studies.
Nagoya University School of Medicine
2013-08
eng
departmental bulletin paper
VoR
https://doi.org/10.18999/nagjms.75.3-4.255
http://hdl.handle.net/2237/18475
https://nagoya.repo.nii.ac.jp/records/16458
10.18999/nagjms.75.3-4.255
http://www.med.nagoya-u.ac.jp/medlib/nagoya_j_med_sci/7534/7534.html
2186-3326
0027-7622
Nagoya Journal of Medical Science
75
3-4
255
261
https://nagoya.repo.nii.ac.jp/record/16458/files/11_Ogawa.pdf
application/pdf
193.2 kB
2018-02-20