2024-03-28T13:36:37Z
https://nagoya.repo.nii.ac.jp/oai
oai:nagoya.repo.nii.ac.jp:00016603
2023-11-16T05:29:37Z
499:508:509:1515
Effects of Palmitic Acid on the Binding of Insulin^-131I to the Rat Liver Mitochondria
KATSUMATA, KAZUO
open access
Effects of palmitic acid, inhibitors, uncouplers, and reduced and oxidized glutathione on the binding of insulin^-131I and 131I to the isolated fresh or aged mitochondria were studied. The binding of insulin^-1311 to the fresh mitochondria was unexpectedly increased to a greater degree with the exogenous addition of palmitic acid, glutathione, acetaldehyde and 2,4-dinitrophenol to the medium. The fact indicated that the intactness of mitochondria was not prerequisite for the binding of insulin^-131I to the isolated mitochondria. The binding of insulin^-131I was compared to the fresh mitochondria and the aged mitochondria. Insulin^-131[ bouud to the aged mitochondria in a lesser degree. It is suggested that the binding of insulin^-131I to the mitochondria might be related to the mitochondrial functional and conformational state. The binding of 131I to the mitochondria was apparently differrnt from that of insulin^-131I. And the binding of 131I was not altered by these uncouplers except in the case of acetaldehyde and ethyl alcohol. This implies that the process of binding of insulin^-131I is different from that of 131I. In the case of 131I, it may have some connections with its ion transport mechanism.
Nagoya University School of Medicine
1970-03
eng
departmental bulletin paper
VoR
https://doi.org/10.18999/nagjms.32.3-4.445
http://hdl.handle.net/2237/18651
https://nagoya.repo.nii.ac.jp/records/16603
10.18999/nagjms.32.3-4.445
2186-3326
0027-7622
Nagoya Journal of Medical Science
32
3-4
445
451
https://nagoya.repo.nii.ac.jp/record/16603/files/v32n34p445_451.pdf
application/pdf
783.7 kB
2018-02-20