2024-03-28T10:24:51Z
https://nagoya.repo.nii.ac.jp/oai
oai:nagoya.repo.nii.ac.jp:00016703
2023-11-16T05:31:28Z
499:508:509:1528
Uncoupling Effect of Tolbutamide on Oxidative Phosphorylation of Liver Mitochondria
KATSUMATA, KAZUO
HAGIHARA, MASAKO
open access
1. The effect of tolbutamide on the oxidation rate, respiratory control index and P/0 ratio of intact rat liver mitochondria was studied. 2. Addition of more than 30 mg% tolbutamide apparently increased the state 4 respiration rate and decreased RCI, whereas, 80 mg% tolbutamide decreased markedly the state 3 respiration rate than that of control. State 4 respiration rate was accelerated by adding 100 mg% (3.8 mM) tolbutamide, while state 3 respiration rate was decreased and P/ 0 ratio was reduced to zero showing complete uncoupling. 3. Uncoupling of oxidative phosphorylation by 100 mg% tolbutamide was similar to that of DNP, i.e., sensitive to the addition of rutamyin, 100 mg% tolbutamide can induce ATPase activity of intact rat liver mitochondria, and induced A TPase activity was reduced to zero by the addition of rutamyin just like DNP. Therefore, uncoupling site by tolbutamide may be close to the DNP sensitive site.
Nagoya University School of Medicine
1973-03
eng
departmental bulletin paper
VoR
https://doi.org/10.18999/nagjms.35.2-4.69
http://hdl.handle.net/2237/18715
https://nagoya.repo.nii.ac.jp/records/16703
10.18999/nagjms.35.2-4.69
http://www.med.nagoya-u.ac.jp/medlib/nagoya_j_med_sci/3524/3524.html
2186-3326
0027-7622
Nagoya Journal of Medical Science
35
2-4
69
77
https://nagoya.repo.nii.ac.jp/record/16703/files/v35n24p69_77.pdf
application/pdf
1.1 MB
2018-02-20