2024-03-29T09:46:15Z
https://nagoya.repo.nii.ac.jp/oai
oai:nagoya.repo.nii.ac.jp:00020880
2023-01-16T04:10:02Z
499:960:1165
Upregulations of P2X3 and ASIC3 involve in hyperalgesia induced by cisplatin administration in rats
Hori, Kiyomi
Ozaki, Noriyuki
Suzuki, Shigeyuki
Sugiura, Yasuo
open access
Cisplatin
TRPV1
TRPV2
P2X3
ASIC3
Hyperalgesia
The role of ion channels expressed in sensory neurons on mechanical and thermal hyperalgesia was examined in a rat model of cisplatin-induced peripheral neuropathy. The rats were injected with 3 mg/kg of cisplatin intraperitoneally once per week for five consecutive weeks. The von Frey test, pin-prick test and plantar test were performed to examine any noxious sensitivity of the skin. The Randall–Selitto test of the gastrocnemius muscle (GM) and the measurement of grip forces were performed to quantify muscle hyperalgesia. Coordination/motor was assessed by Rota-rod testing. Expressions of the ion channels TRPV1, TRPV2, P2X3 and ASIC3 were examined in dorsal root ganglion (DRG) neurons and the muscle afferent neurons innervating GM. Effects of antagonists against either P2X3 or ASICs on behavioral responses were evaluated. Mechanical hyperalgesia and allodynia of both skin and muscle were observed in cisplatin-treated animals. Expressions of TRPV2, P2X3, and ASIC3 increased in all DRG neurons. In addition, expressions of P2X3 and ASIC3 also increased in muscle afferent neurons in DRGs. Antagonists against P2X3,2/3 and ASICs showed a suppressive effect on both skin and muscle hyperalgesia induced by cisplatin administration. Upregulation of TRPV2, P2X3, and ASIC3 may play important roles in the mechanical hyperalgesia induced by cisplatin. Furthermore, cisplatin treatment also induced muscle hyperalgesia in muscle afferent neurons in connection with the upregulation of P2X3 and ASIC3.
名古屋大学博士学位論文 学位の種類 : 博士(医学)(課程) 学位授与年月日:平成22年4月30日 堀紀代美氏の博士論文として提出された
Elsevier
2010-04-30
eng
doctoral thesis
http://hdl.handle.net/2237/23011
https://nagoya.repo.nii.ac.jp/records/20880
https://doi.org/10.1016/j.pain.2010.03.005
0304-3959
PAIN
149
2
393
405
甲第8878号
博士(医学)
2010-04-30
13901
名古屋大学
Nagoya University
https://nagoya.repo.nii.ac.jp/record/20880/files/k8878_thesis.pdf
application/pdf
5.7 MB
2018-02-21