2024-03-29T07:33:43Z
https://nagoya.repo.nii.ac.jp/oai
oai:nagoya.repo.nii.ac.jp:00022928
2023-01-16T04:11:36Z
320:321:322
Shape-controlled high cell-density microcapsules by electrodeposition
Liu, Zeyang
Takeuchi, Masaru
Nakajima, Masahiro
Hasegawa, Yasuhisa
Huang, Qiang
Fukuda, Toshio
open access
© 2016. This manuscript version is made available under the CC-BY-NC-ND 4.0 license http://creativecommons.org/licenses/by-nc-nd/4.0/
Cell encapsulation within alginate-poly-l-lysine (PLL) microcapsules has been developed to provide a miniaturized three-dimensional (3D) microenvironment with an aqueous core while promoting development of encapsulated cells into high cell-density structures. In this paper, a novel method for fabricating shape-controlled alginate-PLL microcapsules to construct 3D cell structures based on electrodeposition method is provided. Two-dimensional Ca-alginate cell-laden gel membranes were electrodeposited onto a micro-patterned electrode and further detached from the electrode. The PLL was coated onto the gel structures to form alginate-PLL complex as an outer shell and sodium citric solution was utilized to melt the internal alginate to achieve miniaturized 3D microcapsules (sphere, cuboid, and rod shape). By this proposed method, rat liver cells (RLC-18) formed multi-cellular aggregates with high cell-density after cultivation for 2 weeks.
Elsevier
2016-06
eng
journal article
AM
http://hdl.handle.net/2237/25108
https://nagoya.repo.nii.ac.jp/records/22928
https://doi.org/10.1016/j.actbio.2016.03.045
1742-7061
Acta Biomaterialia
37
93
100
https://nagoya.repo.nii.ac.jp/record/22928/files/Paper_for_nagoya.pdf
application/pdf
2.5 MB
2018-06-01