2024-03-28T15:25:42Z
https://nagoya.repo.nii.ac.jp/oai
oai:nagoya.repo.nii.ac.jp:00022971
2023-01-16T04:11:31Z
499:500:501
Protein arginine methyltransferase 5 is associated with malignant phenotype and peritoneal metastasis in gastric cancer
Kanda, Mitsuro
Shimizu, Dai
Fujii, Tsutomu
Tanaka, Haruyoshi
Shibata, Masahiro
Iwata, Naoki
Hayashi, Masamichi
Kobayashi, Daisuke
Tanaka, Chie
Yamada, Suguru
Nakayama, Goro
Sugimoto, Hiroyuki
Koike, Masahiko
Fujiwara, Michitaka
Kodera, Yasuhiro
open access
Identification of novel gastric cancer (GC)-related molecules is necessary to improve management of patients with GC in both diagnostic and therapeutic aspects. The aim of the present study was to determine whether protein arginine methyltransferase 5 (PRMT5) acts as an oncogene in the progression of GC and whether it serves as a novel diagnostic marker and therapeutic target. We conducted global expression profiling of GC cell lines and RNA interference experiments to evaluate the effect of PRMT5 expression on the phenotype of GC cells. We analysed tissues of 179 patients with GC to assess the association of PRMT5 mRNA levels with clinicopathological factors. Differential expression of PRMT5 mRNA by GC cell lines correlated positively with the levels of GEMIN2, STAT3 and TGFB3. PRMT5 knockdown reduced the proliferation, invasion and migration of a GC cell line. PRMT5 mRNA levels were significantly higher in GC tissues than the corresponding adjacent normal tissues and were independent of tumour depth, differentiation and lymph node metastasis. High PRMT5 expression was an independent risk factor of positive peritoneal lavage cytology (odds ratio 3.90, P=0.003) and decreased survival. PRMT5 enhances the malignant phenotype of GC cell lines and its expression in gastric tissues may serve as a biomarker for patient stratification and a potential target of therapy.
Spandidos Publications
2016-09
eng
journal article
VoR
http://hdl.handle.net/2237/25152
https://nagoya.repo.nii.ac.jp/records/22971
https://doi.org/10.3892/ijo.2016.3584
1019-6439
International Journal of Oncology
49
3
1195
1202
https://nagoya.repo.nii.ac.jp/record/22971/files/PRMT5-MK_2016_IJO.pdf
application/pdf
537.0 kB
2017-02-01