2024-03-28T10:28:04Z
https://nagoya.repo.nii.ac.jp/oai
oai:nagoya.repo.nii.ac.jp:00024057
2023-01-16T04:14:14Z
336:695:696
Hyperfine Sublevel Correlation Spectroscopy Studies of Iron–Sulfur Cluster in Rieske Protein from Green Sulfur Bacterium Chlorobaculum tepidum
Nagashima, Hiroki
Kishimoto, Hiraku
Mutoh, Risa
Terashima, Naotaka
Oh-oka, Hirozo
Kurisu, Genji
Mino, Hiroyuki
open access
“This document is the Accepted Manuscript version of a Published Work that appeared in final form in [The Journal of Physical Chemistry B], copyright © American Chemical Society after peer review and technical editing by the publisher. To access the final edited and published work see [http://pubs.acs.org/articlesonrequest/AOR-aI6FDDWFX3BNHMpYkfAK].”
The magnetic properties of the Rieske protein purified from Chlorobaculum tepidum were investigated using electron paramagnetic resonance and hyperfine sublevel correlation spectroscopy (HYSCORE). The g-values of the Fe2S2 center were gx = 1.81, gy = 1.90, and gz = 2.03. Four classes of nitrogen signals were obtained by HYSCORE. Nitrogens 1 and 2 had relatively strong magnetic hyperfine couplings and were assigned as the nitrogen directly ligated to Fe. Nitrogens 3 and 4 had relatively weak magnetic hyperfine couplings and were assigned as the other nitrogen of the His ligands and peptide nitrogen connected to the sulfur atom via hydrogen bonding, respectively. The anisotropy of nitrogen 3 reflects the different spin density distributions on the His ligands, which influences the electron transfer to quinone.
ACS Publications
2017-03-02
eng
journal article
AM
http://hdl.handle.net/2237/26261
https://nagoya.repo.nii.ac.jp/records/24057
https://doi.org/10.1021/acs.jpcb.6b12968
1520-6106
The Journal of Physical Chemistry B
121
12
2543
2553
https://nagoya.repo.nii.ac.jp/record/24057/files/resub_Rieske_manuscript_forlibrarydocx.pdf
application/pdf
1.4 MB
2018-03-02