2024-03-29T11:54:18Z
https://nagoya.repo.nii.ac.jp/oai
oai:nagoya.repo.nii.ac.jp:00026086
2023-01-16T04:28:04Z
1935:1936:1937
Chemical hijacking of auxin signaling with an engineered auxin–TIR1 pair
Uchida, Naoyuki
Takahashi, Koji
Iwasaki, Rie
Yamada, Ryotaro
Yoshimura, Masahiko
Endo, Takaho A
Kimura, Seisuke
Zhang, Hua
Nomoto, Mika
Tada, Yasuomi
Kinoshita, Toshinori
Itami, Kenichiro
Hagihara, Shinya
Torii, Keiko U
open access
The phytohormone auxin indole-3-acetic acid (IAA) regulates nearly all aspects of plant growth and development. Despite substantial progress in our understanding of auxin biology, delineating specific auxin response remains a major challenge. Auxin regulates transcriptional response via its receptors, TIR1 and AFB F-box proteins. Here we report an engineered, orthogonal auxin–TIR1 receptor pair, developed through a bump-and-hole strategy, that triggers auxin signaling without interfering with endogenous auxin or TIR1/AFBs. A synthetic, convex IAA (cvxIAA) hijacked the downstream auxin signaling in vivo both at the transcriptomic level and in specific developmental contexts, only in the presence of a complementary, concave TIR1 (ccvTIR1) receptor. Harnessing the cvxIAA–ccvTIR1 system, we provide conclusive evidence for the role of the TIR1-mediated pathway in auxin-induced seedling acid growth. The cvxIAA–ccvTIR1 system serves as a powerful tool for solving outstanding questions in auxin biology and for precise manipulation of auxin-mediated processes as a controllable switch.
ファイル公開:2018-07-22
nature
2018-01-22
eng
journal article
AM
http://hdl.handle.net/2237/00028291
https://nagoya.repo.nii.ac.jp/records/26086
https://doi.org/10.1038/nchembio.2555
1552-4450
Nature Chemical Biology
14
3
299
305
https://nagoya.repo.nii.ac.jp/record/26086/files/Uchida_et_al.pdf
application/pdf
3.7 MB
2018-07-22