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https://nagoya.repo.nii.ac.jp/oai
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2023-01-16T04:54:44Z
643:666:667
The mRNA-binding protein Serbp1 as an auxiliary protein associated with mammalian cytoplasmic ribosomes
Muto, Akiko
Sugihara, Yoshihiko
Shibakawa, Minami
Oshima, Kenzi
Matsuda, Tsukasa
Nadano, Daita
open access
This is the peer reviewed version of the following article: [Muto A, Sugihara Y, Shibakawa M, Oshima K, Matsuda T, Nadano D. The mRNA‐binding protein Serbp1 as an auxiliary protein associated with mammalian cytoplasmic ribosomes. Cell Biochem Funct. 2018;36:312–322. https://doi.org/10.1002/cbf.3350], which has been published in final form at [https://doi.org/10.1002/cbf.3350]. This article may be used for non-commercial purposes in accordance with Wiley Terms and Conditions for Use of Self-Archived Versions.
cytoplasmic ribosome
gene expression
isoform
malignant tumour
mammal
mRNA‐binding protein
polysome
translational control
While transcription plays an obviously important role in gene expression, translation has recently been emerged as a key step that defines the composition and quality of the proteome in the cell of higher eukaryotes including mammals. Selective translation is supposed to be regulated by the structural heterogeneity of cytoplasmic ribosomes including differences in protein composition and chemical modifications. However, the current knowledge on the heterogeneity of mammalian ribosomes is limited. Here, we report mammalian Serbp1 as a ribosome‐associated protein. The translated products of Serbp1 gene, including the longest isoform, were found to be localized in the nucleolus as well as in the cytoplasm. Subcellular fractionation indicated that most of cytoplasmic Serbp1 molecules were precipitated by ultracentrifugation. Proteomic analysis identified Serbp1 in the cytoplasmic ribosomes of the rodent testis. Polysome profiling suggested that Serbp1, as a component of the small 40S subunit, was included in translating ribosomes (polysomes). Cosedimentation of Serbp1 with the 40S subunit was observed after dissociation of the ribosomal subunits. Serbp1 was also included in the ribosomes of human cancer cells, which may lead to a mechanistic understanding of an emerging link between Serbp1 and tumour progression.
ファイル公開:2019/08/01
Wiley
2018-08
eng
journal article
AM
http://hdl.handle.net/2237/00028538
https://nagoya.repo.nii.ac.jp/records/26335
https://doi.org/10.1002/cbf.3350
0263-6484
Cell Biochemistry and Function
36
6
312
322
https://nagoya.repo.nii.ac.jp/record/26335/files/Serbp1-201808-op.pdf
application/pdf
1.3 MB
2019-08-01