2024-03-28T19:07:28Z
https://nagoya.repo.nii.ac.jp/oai
oai:nagoya.repo.nii.ac.jp:00028340
2023-01-16T04:20:55Z
499:500:501
Spread of seb-Positive Methicillin-Resistant Staphylococcus aureus SCCmec Type II-ST764 Among Elderly Japanese in Nonacute Care Settings
Kawamura, Kumiko
Kitaoka, Kazuki
Kimura, Kouji
Wachino, Jun-ichi
Kondo, Takaaki
Iinuma, Yoshitsugu
Murakami, Nobuo
Fujimoto, Shuhei
Arakawa, Yoshichika
open access
“Final publication is available from Mary Ann Liebert, Inc., publishers http://dx.doi.org/10.1089/mdr.2018.0337”.
methicillin-resistant Staphylococcus aureus
SCCmec type
toxin gene
long-term care facility
ST764
We investigated the prevalence and molecular epidemiology of methicillin-resistant Staphylococcus aureus (MRSA) among 356 residents of nine long-term care facilities (LTCFs) in Japan during 2015 and 2017. In total, 800 specimens were tested and 39 MRSA isolates were recovered from 31 (8.71%) residents. PCR-based open reading frame typing (POT) and pulsed-field gel electrophoresis typing were performed for the 39 MRSA isolates; five of them showing identical pulsotypes, and POT scores were excluded in further analysis. Staphylococcal cassette chromosome mec (SCCmec) typing, multilocus sequence typing, and toxin gene detection were performed for one representative MRSA isolate per resident. Among the 34 unrelated MRSA isolates, 15 (44.1%) and 19 (55.9%) were of SCCmec types II and IV, respectively, and belonged to seven sequence types (STs). Among the 15 SCCmec II isolates, 11 (73.3%), 3, and 1 belonged to ST764 (clonal complex [CC]5), ST5 (CC5), and ST630 (CC8), respectively. Among the 19 SCCmec IV isolates, 13 (68.4%), 3, 2, and 1 belonged to ST1 (CC1), ST474 (CC1), ST8 (CC8), and ST380 (CC8), respectively. Among the 14 CC5 lineage-SCCmec II isolates, one ST5 isolate and 7 of the 11 ST764 isolates (63.6%) carried seb gene, and 14 (87.5%) of 16 CC1 lineage-SCCmec IV isolates had sea gene (p < 0.05). The results indicate that the seb-positive SCCmec type II-ST764 clone has spread in Japanese LTCF environments. As LTCF residents have multiple comorbidities and increased susceptibility to infections, it is necessary to monitor MRSA colonization in LTCFs through periodic screening to prevent dissemination.
Online Ahead of Print:March 21, 2019 ファイル公開:2020-03-21
Mary Ann Liebert
2019-07-11
eng
journal article
AM
http://hdl.handle.net/2237/00030535
https://nagoya.repo.nii.ac.jp/records/28340
https://doi.org/10.1089/mdr.2018.0337
1076-6294
Microbial Drug Resistance
25
6
915
924
https://nagoya.repo.nii.ac.jp/record/28340/files/MDR-2018-0337-Kawamura_1P.pdf
application/pdf
463.2 kB
2020-03-21