2024-03-29T06:45:46Z
https://nagoya.repo.nii.ac.jp/oai
oai:nagoya.repo.nii.ac.jp:00030335
2023-01-16T04:23:43Z
499:500:501
A new method with an explant culture of the utricle for assessing the influence of exposure to low-frequency noise on the vestibule
Ohgami, Nobutaka
He, Tingchao
Oshino-Negishi, Reina
Gu, Yishuo
Li, Xiang
Kato, Masashi
open access
This is an Accepted Manuscript of an article published by Taylor & Francis Group in Journal of Toxicology and Environmental Health, Part A on 05/04/2020, available online: http://www.tandfonline.com/10.1080/15287394.2020.1746945.
low frequency noise
risk assessment
vestibule
otoconial membrane
balance
Health risks attributed to low-frequency noise (LFN) exposure are a serious global issue. Therefore, the development of a method for a prevention based upon risk assessments for LFN is important. Previously in vivo exposure of mice to LFN at 100 Hz, 95 dB for 1 hr produced imbalance with breakage of the otoconial membrane, which covers hair cells as well as impaired activity of hair cells in the vestibule. However, methods for inhibition of LFN-mediated imbalance have not been developed. At present, there are no apparent techniques available with in vitro or ex vivo assessments to evaluate LFN-mediated imbalance by direct administration of preventive chemicals into the vestibule. Our findings demonstrated the usefulness of an explant culture of the utricle with a fluorescent styryl dye, FM1-43FX. In addition, examination of the morphology of the otoconial membrane with explant cultures of utricles was conducted to determine the risk of LFN. Ex vivo exposure of the utricle to LFN at 100 Hz, 95 dB for 1 hr induced breaks in the otoconial membrane as well as decreased uptake of FM1-43FX in hair cells. Taken together, the results of this study provide a novel technique for assessing the risk of LFN exposure using an ex vivo experiment.
ファイル公開:2021/04/05
Taylor & Francis
2020-04-05
eng
journal article
AM
http://hdl.handle.net/2237/00032521
https://nagoya.repo.nii.ac.jp/records/30335
https://doi.org/10.1080/15287394.2020.1746945
1528-7394
1087-2620
Journal of Toxicology and Environmental Health, Part A
83
5
215
218
https://nagoya.repo.nii.ac.jp/record/30335/files/UTEH-2020-0106_R1_repository.pdf
application/pdf
4.8 MB
2021-04-05