2024-03-28T23:06:11Z
https://nagoya.repo.nii.ac.jp/oai
oai:nagoya.repo.nii.ac.jp:00031023
2023-01-16T04:30:25Z
2081:2082:2083
Structural insights into gap junction channels boosted by cryo-EM
Oshima, Atsunori
open access
© 2020. This manuscript version is made available under the CC-BY-NC-ND 4.0 license http://creativecommons.org/licenses/by-nc-nd/4.0/
Regulating intercellular communication is essential for multicellular organisms. Gap junction channels are the major components mediating this function, but the molecular mechanisms underlying their opening and closing remain unclear. Single-particle cryo-electron microscopy (cryo-EM) is a powerful tool for investigating high-resolution protein structures that are difficult to crystallize, such as gap junction channels. Membrane protein structures are often determined in a detergent solubilized form, but lipid bilayers provide a near native environment for structural analysis. This review focuses on recent reports of gap junction channel structures visualized by cryo-EM. An overview of the differences observed in gap junction channel structures in the presence and absence of lipids is described, which may contribute to elucidating the regulation mechanisms of gap junction channel function.
ファイル公開:2021-08-01
Elsevier
2020-08
eng
journal article
AM
http://hdl.handle.net/2237/00033206
https://nagoya.repo.nii.ac.jp/records/31023
https://doi.org/10.1016/j.sbi.2020.03.008
0959-440X
Current Opinion in Structural Biology
63
42
48
https://nagoya.repo.nii.ac.jp/record/31023/files/Oshima_2020COSBT.pdf
application/pdf
348.4 kB
2021-08-01