2024-03-29T06:49:59Z
https://nagoya.repo.nii.ac.jp/oai
oai:nagoya.repo.nii.ac.jp:02001587
2023-01-16T05:02:54Z
336:695:696
A type 2C protein phosphatase activates high-affinity nitrate uptake by dephosphorylating NRT2.1
Ohkubo, Yuri
Kuwata, Keiko
Matsubayashi, Yoshikatsu
open access
The nitrate transporter NRT2.1, which plays a central role in high-affinity nitrate uptake in roots, is activated at the post-translational level in response to nitrogen (N) starvation. However, the critical enzymes required for the post-translational activation of NRT2.1 remain to be identified. Here, we show that a type 2C protein phosphatase, designated CEPD-induced phosphatase (CEPH), activates high-affinity nitrate uptake by directly dephosphorylating Ser501 of NRT2.1, a residue that functions as a negative phospho-switch in Arabidopsis. CEPH is predominantly expressed in epidermal and cortex cells in roots and is upregulated by N starvation via a CEPDL2/CEPD1/2-mediated long-distance signalling from shoots. The loss of CEPH leads to marked decreases in high-affinity nitrate uptake, tissue nitrate content and plant biomass. Collectively, our results identify CEPH as a crucial enzyme in the N-starvation-dependent activation of NRT2.1 and provide molecular and mechanistic insights into how plants regulate high-affinity nitrate uptake at the post-translational level in response to the N environment.
nature
2021-11-11
2021-03
eng
journal article
AM
http://hdl.handle.net/2237/0002001587
https://nagoya.repo.nii.ac.jp/records/2001587
https://doi.org/10.1038/s41477-021-00870-9
2055-0278
Nature Plants
7
3
310
316
https://nagoya.repo.nii.ac.jp/record/2001587/files/CEPH_manuscript.pdf
application/pdf
3.9 MB
2021-11-11