2024-03-29T09:47:46Z
https://nagoya.repo.nii.ac.jp/oai
oai:nagoya.repo.nii.ac.jp:02001629
2023-05-08T00:29:05Z
886:2329:2330
Rice MEDIATOR25, OsMED25, is an essential subunit for jasmonate-mediated root development and OsMYC2-mediated leaf senescence
Suzuki, Go
Lucob-Agustin, Nonawin
Kashihara, Keita
Fujii, Yumi
Inukai, Yoshiaki
Gomi, Kenji
open access
© 2021. This manuscript version is made available under the CC-BY-NC-ND 4.0 license http://creativecommons.org/licenses/by-nc-nd/4.0/
Jasmonate
Leaf senescence
OsMYC2
Rice
Root
The Mediator multiprotein complex acts as a universal adaptor between transcription factors (TFs) and RNA polymerase II. MEDIATOR25 (MED25) has an important role in jasmonic acid (JA) signaling in Arabidopsis. However, no research has been conducted on the role of MED25 in JA signaling in rice, which is one of the most important food crops globally and is a model plant for molecular studies in other monocotyledonous species. In the present study, we isolated the loss-of function mutant of MED25, osmed25, through the map-based cloning and phenotypic complementation analysis by the introduction of OsMED25 and investigated the role of OsMED25 in JA signaling in rice. The osmed25 mutants had longer primary (seminal) roots than those of the wild-type (WT) and exhibited JA-insensitive phenotypes. S-type lateral root densities in osmed25 mutants were lower than those in the WT, whereas L-type lateral root densities in osmed25 mutants were higher than those in the WT. Furthermore, the osmed25 mutants retarded JA-regulated leaf senescence under dark-induced senescence. Mutated osmed25 protein could not interact with OsMYC2, which is a positive TF in JA signaling in rice. The expression of JA-responsive senescence-associated genes was not upregulated in response to JA in the osmed25 mutants. The results suggest that OsMED25 participates in JA-mediated root development and OsMYC2-mediated leaf senescence in rice.
Elsevier
2023-05-01
2021-05
eng
journal article
AM
http://hdl.handle.net/2237/0002001629
https://nagoya.repo.nii.ac.jp/records/2001629
https://doi.org/10.1016/j.plantsci.2021.110853
01689452
Plant Science
306
110853
https://nagoya.repo.nii.ac.jp/record/2001629/files/20210211_Suzuki_et_al.pdf
application/pdf
2 MB
2023-05-01