2024-03-28T18:25:15Z
https://nagoya.repo.nii.ac.jp/oai
oai:nagoya.repo.nii.ac.jp:02005582
2023-04-24T00:34:39Z
1213:1620:1621:1681884086109
筋収縮とインスリンが骨格筋のglycolysisに及ぼす影響について : Microdialysis法による検討
Effects of muscle contraction and insulin on glycolysis in rats : Use of microdialysis technique
楊, 衛平
YANG, Wei-Ping
呉, 瑋
WU, Wei
押田, 芳治
OSHIDA, Yoshiharu
大沢, 功
OHSAWA, Isao
佐藤, 寿一
SATO, Juichi
佐藤, 祐造
SATO, Yuzo
open access
In order to elucidate the effects of skeletal muscle contraction and insulin on glucose uptake in exercised muscle, we studied 19 male Wistar rats by using microdialysis technique in both femoral muscles and jugular vein. Under the continuous somatostatin infusion (0.5 μg/kg·min) and bolus infusion of anti-insulin serum (0.3ml), which induced a complete suppresson of insulin secretion (plasma insulin levels were less than 3μU/ml), these rats were divided into 2 groups. One was that both femoral muscles were stimulated electrically (S). The other was that both thighs were kept resting without stimulation (C). The lactate concentration in dialysate were determined every 15minutes and the dialysate lactate concentration in muscle was a measure of glucose uptake. After somatostatin infusion (2 hours), insulin was infused continuously(6.0mU/kg·min) in both groups. The following results were obtained: 1. In S group., the dialysate lactate concentrations in muscle were significantly higher than that in jugular vein under the condition of complete suppression of insulin secretion. 2. In C group, the dialysate lactate levels in muscle were significantly lower than that in jugular vein under the above condition. 3. During inslin infusion, the increase in dialysate lactate concentration in muscle of S group was almost similar to that of C group. From these results, it could be confirmed that exercised muscle results in a large increase in glucose uptake even if insulin secretion is suppressed completely, and that insulin acts as an additive effect in exercised muscle.
名古屋大学総合保健体育科学センター
The Research Center of Health, Physical Fitness and Sports, Nagoya University
1992-03-30
jpn
departmental bulletin paper
VoR
https://doi.org/10.18999/njhpfs.15.1.23
http://hdl.handle.net/2237/0002005582
https://nagoya.repo.nii.ac.jp/records/2005582
10.18999/njhpfs.15.1.23
0289-5412
総合保健体育科学
Nagoya Journal of Health, Physical Fitness & Sports
15
1
23
25
https://nagoya.repo.nii.ac.jp/record/2005582/files/njhpfs_15_1_23.pdf
application/pdf
1.8 MB
2023-04-24