2024-03-29T10:35:37Z
https://nagoya.repo.nii.ac.jp/oai
oai:nagoya.repo.nii.ac.jp:00001372
2023-01-26T05:58:38Z
329:333:334:335
Analysis of Interieukin-8 Gene Promoter function in Human Osteoblast-like Cells : Regulation by Ca^<2+>-signaling and Cyclosporin A
MITSUYAMA, Hirohito
3124
KAMBE, Fukushi
3125
MURAKAMI, Ryuichiro
3126
ISHIGURO, Naoki
3127
SEO, Hisao
3128
interleukin-8
cyclosporin A
human osteoblast
2002-12
We previously reported that an increase in intracellular calcium by calcium ionophore (A23187) and 4p-phorbol-12β- myristate-13α-acetate (PMA) induced the expression of IL-8 mRNA in human osteoblast-like HOS-TE85 cells and this induction was markedly suppressed by cyclosporin A (CsA). In this study we investigated whether the regulation by A23187, PMA and CsA was occured at a transcriptional level by reporter gene assays. A promoter region spanning from -1460 to +40 of IL-8 gene was PCR- amplified and inserted upstream of a luciferase reporter gene. Various deletion mutants were also constructed. Transfection of the plasmids into HOS-TE85 cells demonstrated that the nucleotides between -133 and -60 base pairs upstream of IL-8 gene are essential and sufficient for its induction by A23187/PMA, and suppression of this induction by CsA.
国立情報学研究所で電子化したコンテンツを使用している。
departmental bulletin paper
Research Institute of Environmental Medicine, Nagoya University
2002-12
Environmental medicine : annual report of the Research Institute of Environmental Medicine, Nagoya University
1/2
46
52
54
http://hdl.handle.net/2237/2782
02870517
eng