2024-03-28T17:26:29Z
https://nagoya.repo.nii.ac.jp/oai
oai:nagoya.repo.nii.ac.jp:00001385
2023-01-26T06:04:34Z
329:333:334:335
Optimal Population of Embryonic Stem Cells in "Hanging Drop" Culture for in-vitro Differentiation to Cardiac Myocytes
MIWA, Keiko
3185
LEE, Jong-Kook
3186
HIDAKA, Kyoko
3187
SHI, Rong-qian
3188
MORISAKI, Takayuki
3189
KODAMA, Itsuo
3190
embryonic stem cell
fluorescence-activated cell sorting
2002-12
Pluripotent embryonic stem (ES) cells differentiate to cardiac myocytes in vitro by many other previous reports demonstrated "hanging-drop" method. In this study, the number of ES cells in each hanging-drop plays an important role in the cultivation of cardiac myocytes. We examined the optimal hanging-drop size to obtain embryonic stem cell-derived cardiac cells (ESCMs) in vitro using specific labeled mouse ES cells (hCGP7) which were stably transfected with the enhanced green fluorescent protein (GFP) under transcriptional control of the cardiac myocyte-specific promoter with fluorescence-activated cell sorting (FACS) analysis. GFP-positive cells were sorted efficiently in the hanging-drop cell number of approximately 800~1600 cells/drop. The present data will be an important element to establish the optimal methods of ES cellular differentiation in vitro.
国立情報学研究所で電子化したコンテンツを使用している。
departmental bulletin paper
Research Institute of Environmental Medicine, Nagoya University
2002-12
Environmental medicine : annual report of the Research Institute of Environmental Medicine, Nagoya University
1/2
46
92
94
http://hdl.handle.net/2237/2795
02870517
eng