2024-03-28T16:14:49Z
https://nagoya.repo.nii.ac.jp/oai
oai:nagoya.repo.nii.ac.jp:00005828
2023-01-16T04:54:38Z
499:500:501
Crucial transcription factors in endoderm and embryonic gut development are expressed in gut-like structures from mouse ES cells
Matsuura, Rie
15464
Kogo, Hiroshi
15465
Ogaeri, Takunori
15466
Miwa, Takashi
15467
Kuwahara, Masaki
15468
Kanai, Yoshiakira
15469
Nakagawa, Takumi
15470
Kuroiwa, Atsushi
15471
Fujimoto, Toyoshi
15472
Torihashi, Shigeko
15473
鳥橋, 茂子
15474
embryonic stem cell
embryoid body
transcription factors
development
gastrointestinal motility
Mouse embryonic stem cells (ES cells) are pluripotent and retain the potential to form an organ similar to gut showing spontaneous contractions in vitro. The morphological features and their formation process using the hanging drop method to compose embryoid bodies (EBs) seem to be similar to those in vivo. To determine whether the same molecular mechanisms are involved in the formation process, the expressions of transcription factors regulating endoderm and gut development in the mouse embryo were examined by in situ hybridization and compared to those in vivo. The expressions of gene products were also examined by immunohistochemistry and their co-localization was analyzed with double staining. The results showed that all factors examined, i.e., Sox17, Id2, HNF3β/Foxa2, and GATA4, were expressed in both EBs and the gut-like structures. Moreover, their expression patterns were similar to those in the mouse embryo. EBs, after the hanging drop and before outgrowth, already expressed all factors co-localized with each other at the EB epithelial structures. These findings suggest that the origin of gut-like structure is determined and formed as the epithelial structure in EB during the hanging drop, and they also indicate that the in vitro system using mouse ES cells mimics the development in vivo and should prove useful in the study of molecular mechanisms for endoderm and gut development.
journal article
Alpha Med Press
2006-03
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Stem Cells
3
24
624
630
http://hdl.handle.net/2237/7444
1066-5099
https://nagoya.repo.nii.ac.jp/record/5828/files/Fig7.pdf
https://nagoya.repo.nii.ac.jp/record/5828/files/Fig6.pdf
https://nagoya.repo.nii.ac.jp/record/5828/files/Fig5.pdf
https://nagoya.repo.nii.ac.jp/record/5828/files/Fig4.pdf
https://nagoya.repo.nii.ac.jp/record/5828/files/Fig3.pdf
https://nagoya.repo.nii.ac.jp/record/5828/files/Fig2.pdf
https://nagoya.repo.nii.ac.jp/record/5828/files/Fig1.pdf
https://nagoya.repo.nii.ac.jp/record/5828/files/Stem_Cells_accepted.pdf
eng
https://doi.org/10.1634/stemcells.2005-0344