2024-03-29T11:36:28Z
https://nagoya.repo.nii.ac.jp/oai
oai:nagoya.repo.nii.ac.jp:00005956
2023-01-26T06:20:17Z
329:333:334:737
The Difference of mRNA Expression of ATP-Sensitive K^+ Channel Subunits in Embryonic and Adult Mouse Heart
Yasui, Kenji
15853
Hojo, Mayumi
15854
Kodama, Itsuo
15855
ATP-sensitive K channel
mRNA
mouse
heart
2003-12
ATP-sensitive K^+(K_<ATP>) channels is expressed in early-stage embryonic heart as well as at the adult stage. K_<ATP> channels are composed of pore-forming (Kir) and regulatory sulfonylurea receptor (SUR) subunits. We studied mRNA expression of Kir6.1, Kir6.2, SUR1, SUR2A, SUR2B and SUR2C mRNAs encoding for K_(ATP) channels in cardiac ventricles of embryonic (9.5 days postcoitum, dpc) and adult mice using RT-PCR. 30 cycles of PCR revealed mRNA expression of all genes at the adult-stage. At 9.5 dpc, Kir6.1 mRNA was undetectable, whereas mRNAs of other subunits were detected. At 9.5 dpc, SUR2A expression was higher than SUR1 and SUR2B. In the adulthood, mRNAs of all subunits were detected. This finding suggests that Kir6.2 is dominant pore-forming subunit from an early embryonic stage. The developmental changes of transcriptional profile of K_(ATP) channels may underlie the different functional properties of the channel in pre-and post-natal period.
国立情報学研究所で電子化したコンテンツを使用している。
departmental bulletin paper
Research Institute of Environmental Medicine, Nagoya University
2003-12
Environmental Medicine : annual report of the Research Institute of Environmental Medicine, Nagoya University
47
35
36
http://hdl.handle.net/2237/7570
0287-0517
eng