2024-03-28T18:23:04Z
https://nagoya.repo.nii.ac.jp/oai
oai:nagoya.repo.nii.ac.jp:00008855
2023-01-16T05:03:58Z
329:330:331
Paracrine factors of vascular endothelial cells facilitate cardiomyocyte differentiation of mouse embryonic stem cells
角, 三和子
24908
Kado, Miwako
24909
李, 鍾國
24910
Lee, Jong-Kook
24911
日高, 京子
24912
Hidaka, Kyoko
24913
三輪, 佳子
24914
Miwa, Keiko
24915
室原, 豊明
24916
Murohara, Toyoaki
24917
笠井, 謙次
24918
Kasai, Kenji
24919
佐賀, 信介
24920
Saga, Shinsuke
24921
森崎, 隆幸
24922
Morisaki, Takayuki
24923
上田, 裕一
24924
Ueda, Yuichi
24925
児玉, 逸雄
24926
Kodama, Itsuo
24927
Embryonic stem cell
Cardiac cell
Vascular endothelial cell
Paracrine factor
Cardiomyogenesis
Nkx2.5
Bone morphogenic protein
Wnt
Cyclooxygenase
Nitric oxide synthase
For myocardial regeneration therapy, the low differentiation capability of functional cardiomyocytes sufficient to replace the damaged myocardial tissue is one of the major difficulties. Using Nkx2.5-GFP knock-in ES cells, we show a new efficient method to obtain cardiomyocytes from embryonic stem (ES) cells. The proportion of GFP-positive cells was significantly increased when ES cells were cultured with a conditioned medium from aortic endothelial cells (ECs), accompanied by upregulation of cardiac-specific genes as well as other mesodermal genes. The promotion was more prominent when EC-conditioned medium was added at an early stage of ES cell differentiation culture (Day 0–3). Inhibitors of bone morphogenic protein (BMP), cyclooxygenase (COX), and nitric oxide synthetase (NO) prevented the promotion of cardiomyogenesis by EC-conditioned medium. These results suggest that supplementation of EC-conditioned medium enables cardiomyocytes to be obtained efficiently through promotion of mesoderm induction, which is regulated by BMP, COX, and NOS.
journal article
Elsevier
2008
application/pdf
Biochemical and Biophysical Research Communications
2
377
413
418
http://hdl.handle.net/2237/10608
0006291X
https://nagoya.repo.nii.ac.jp/record/8855/files/Paracrine_factors_of_vascular_endothelial_cells_facilitate_cardiomyocyte_differentiation_of_mouse_embryonic_stem_cells.pdf
eng
https://doi.org/10.1016/j.bbrc.2008.09.160
This is the author's version of work accepted for publication by Elsevier. Changes resulting from the publishing process, including peer review, editing, corrections, structural formatting and other quality control mechanisms, may not be reflected in this document. Changes may have been made to this work since it was submitted for publication. A definitive version was subsequently published in [Biochemical and Biophysical Research Communications, Article in Press, DOI:10.1016/j.bbrc.2008.09.160]