2024-03-29T08:48:44Z
https://nagoya.repo.nii.ac.jp/oai
oai:nagoya.repo.nii.ac.jp:00013457
2023-11-16T06:36:58Z
499:508:509:1359
STRONG ANTIBODY REACTION AGAINST GLYCOSPHINGOLIPIDS INJECTED IN LIPOSOMEEMBEDDED FORMS IN β3GN-T5 KNOCKOUT MICE
FAN, XIAOYAN
42185
KONDO, YUJI
42186
TOKUDA, NORIYO
42187
OHMI, YUHSUKE
42188
ANDO, REIKO
42189
UMEZU, TOMOKAZU
42190
ZHANG, QING
42191
FURUKAWA, KEIKO
42192
SHIBATA, KIYOSUMI
42193
TOGAYACHI, AKIRA
42194
NARIMATSU, HISASHI
42195
OKAJIMA, TETSUYA
42196
KIKKAWA, KOJI
42197
FURUKAWA, KOICHI
42198
Immunization
Antibody
Knockout
Glycolipids
Gangliosides
Liposome
2011-08
It is known that mutant mice of the β-1,3-N-acetylglucosaminyltransferase gene (β3Gn-T5) respond well to T-cell dependent and independent antigens. Here, we examined the effectiveness of anti-ganglioside antibody generation by immunization of β3Gn-T5 mutant mice with liposome-embedded glycosphingolipids such as GD1a and GT1b. Consequently, the mutant mice showed a more efficient generation of anti-GD1a or anti-GT1b antibodies than wild-type mice in an enzyme-linked immunosorbent assay using sera during immunization. Thus, the β3Gn-T5 deficient mutant mice proved more responsive than wild-type mice to not only protein antigens, but also to carbohydrates in glycolipids. Furthermore, about 50% of monoclonal antibodies generated using splenocytes of the immunized mutant mice were of the IgG class. Besides general high responsiveness to proteins and glycolipids, it could be expected that the mutant mice of β3Gn-T5 would be useful in the generation of monoclonal antibodies towards lacto-/neolacto-series glycolipids, since these mutants lack lacto-/neolacto-series glycolipids. In fact, they showed a good serum response in immuno-fluorescence assay with cultured living cells when immunized by glycolipids extracted from ovarian cancer cell lines. These results suggested that β3Gn-T5 mutant mice are useful for the generation of anti-glycolipid antigens with lacto-/neolacto-core structures expressed in cancer cells.
departmental bulletin paper
Nagoya University School of Medicine
2011-08
Nagoya Journal of Medical Science
3-4
73
137
146
http://hdl.handle.net/2237/15356
2186-3326
0027-7622
eng
http://www.med.nagoya-u.ac.jp/medlib/nagoya_j_med_sci/7334/7334.html