2024-03-28T17:18:28Z
https://nagoya.repo.nii.ac.jp/oai
oai:nagoya.repo.nii.ac.jp:00015448
2023-11-16T05:48:51Z
499:508:509:1456
Some Properties of Amine Oxidase from Soybean Seedlings
MATSUMOTO, TAKATOSHI
98713
NIMURA, YUJI
98714
FURUTA, TAMAKI
98715
HAYAKAWA, NAOKAZU
98716
ASAI, MASANORI
98717
KUROKAWA, YOSHIE
98718
HATTORI, TATSUO
98719
IYOMASA, YOTARO
98720
Soybean seedling
Amine oxidase
Diamine
Polyamine
1984-03
The purpose of the present study was to clarify the properties of amine oxidase partially purified from soybean seedlings, which the authors used as an enzyme sample for the assay of diamines (putrescine, cadaverine) and polyamines (spermidine, spermine). The enzyme activity was highest with cadaverine followed by putrescine, spermidine, 1,7-diaminoheptane and spermine. The optimum pH was 7.8 for putrescine and cadaverine and 8.0 for spermidine and spermine. Carbonyl reagents, such as semicarbazide, and chelating reagents, such as cuprizone, were effective inhibitots of soybean seedling amine oxidase (SSAO), suggesting that Cu++ and pyridoxal may be cofactors for SSAO. Therefore, SSAO appeared to be a diamine oxidase (EC 1.4.3.6). It was stable at 50°C for at least 2 hours, and the apparent Km values for all diamines and polyamines were extremely low. Since SSAO is present in great quantities in soybean seedlings, which can be easily obtained in every season in Japan, it is very suitable as an enzyme sample for the assay of diamines and polyamines.
departmental bulletin paper
Nagoya University School of Medicine
1984-03
Nagoya Journal of Medical Science
1-4
46
87
94
2186-3326
0027-7622
eng
http://www.med.nagoya-u.ac.jp/medlib/nagoya_j_med_sci/4614/4614.html