2024-03-29T11:36:41Z
https://nagoya.repo.nii.ac.jp/oai
oai:nagoya.repo.nii.ac.jp:00015455
2023-11-16T05:49:08Z
499:508:509:1457
Quantitation of Red-Cell-Bound IgG in Normal and Pathologic States by an Enzyme Immunoassay (EIA) Technique
HIRANO, AKIHITO
47074
YAMADA, HIDEO
47075
KATO, KANEFUSA
47076
Enzyme Immunoassay
Red-Cell-Bound IgG
AIHA
1985-01
A simple EIA technique for the quantitation of red-cell-bound immunoglobulin (IgG) was devised and applied to normal and pathologic states, including autoimmune hemolytic anemia (AIHA) and other disorders. The mean red-cell-bound IgG value for normal subjects was 73.2 ± 25.8 ng/l0^10 red cells (mean ± ISD). Fifteen patients with AIHA had an increased level of red-cell-bound IgG ranging from 109 to 10,000 ng/l0^10 red cells. The concentration of red-cell-bound IgG in AIHA patients was well correlated with the percentage of reticulocytes in the peripheral blood and showed a rapid fall after the initiation of steroid therapy. Red-cell-bound IgG levels in Fisher-Evans syndrome, idiopathic thrombocytopenic purpura (ITP), systemic lupus erythematosus (SLE), rheumatoid arthritis (RA) and IgG myeloma measured by this technique are also presented. A significant correlation was found between red-cell-bound IgG levels and serum IgG levels in normal subjects and in patients with these pathologic conditions, except in the cases of AIHA and Fisher-Evans syndrome. The quantitation of red-cell-bound IgG by this EIA technique is of great value for clarifying the pathophysiologic significance of red-cell-bound IgG in health and disease.
departmental bulletin paper
Nagoya University School of Medicine
1985-01
Nagoya Journal of Medical Science
1-2
47
5
16
http://hdl.handle.net/2237/17472
2186-3326
0027-7622
eng
http://www.med.nagoya-u.ac.jp/medlib/nagoya_j_med_sci/4712/4712.html