2024-03-29T07:38:30Z
https://nagoya.repo.nii.ac.jp/oai
oai:nagoya.repo.nii.ac.jp:00015505
2023-11-16T05:51:20Z
499:508:509:1465
The Effect of Hypophysectomy on Proluminal Movement of 3H-Androgens Across the Epididymal Epithelium in the Rat
YAMAMOTO, MASANORI
47305
TSUJI, YOSHIKAZU
47306
NAGAI, TATSUYA
47307
MIYAKE, KOJI
47308
Testosterone
Epididymis
Hypophysectomy
Micropuncture
Microperifusion
1992-03
The effect of hypophysectomy on transepithelial movement of 3H-androgen in the rat epididymis was examined by using in vivo microperifusion of 3H-testosterone followed by in vivo micropuncture to obtain peritubular and intraluminal fluids. Experiments were performed on aminals without hypophysectomy or on aminals 5-6 days after hypophysectomy and 9-10 days after hypophysectomy. Tubules were perifused with Minimum Essential Medium containing 3H-testosterone. 14C-polyethyleneglycol was included in the perifusion fluid as a marker for contamination of the intraluminal fluid by peritubular fluid. Radioactivity of isotopes in the interstitial and intraluminal fluid was determined at 1 and 2 hours after perifusion and the percentage of peritubular isotopes appearing in the intraluminal fluid was determined. A sperm concentration microassay was performed on micropuncture samples from the epididymal tubules to assess testicular contribution to the lumen content. Proluminal movement of 3H-androgen and intratubular sperm concentrations in the caput epididymal tubules of rats 9-10 days after hypophysectomy were significantly decreased. Proluminal movement of 3H-androgen and intratubular sperm concentrations in the cauda epididymal tubules of rats 9-10 days after hypophysectomy were significantly increased. These results suggest that proluminal androgen movement is controlled by the presence of some testicular product in the epididymal lumen.
departmental bulletin paper
Nagoya University School of Medicine
1992-03
Nagoya Journal of Medical Science
1-4
54
67
76
http://hdl.handle.net/2237/17523
2186-3326
0027-7622
eng
http://www.med.nagoya-u.ac.jp/medlib/nagoya_j_med_sci/5414/5414.html