2024-03-28T14:15:43Z
https://nagoya.repo.nii.ac.jp/oai
oai:nagoya.repo.nii.ac.jp:00022928
2023-01-16T04:11:36Z
320:321:322
Shape-controlled high cell-density microcapsules by electrodeposition
Liu, Zeyang
67524
Takeuchi, Masaru
67525
Nakajima, Masahiro
67526
Hasegawa, Yasuhisa
67527
Huang, Qiang
67528
Fukuda, Toshio
67529
Cell encapsulation within alginate-poly-l-lysine (PLL) microcapsules has been developed to provide a miniaturized three-dimensional (3D) microenvironment with an aqueous core while promoting development of encapsulated cells into high cell-density structures. In this paper, a novel method for fabricating shape-controlled alginate-PLL microcapsules to construct 3D cell structures based on electrodeposition method is provided. Two-dimensional Ca-alginate cell-laden gel membranes were electrodeposited onto a micro-patterned electrode and further detached from the electrode. The PLL was coated onto the gel structures to form alginate-PLL complex as an outer shell and sodium citric solution was utilized to melt the internal alginate to achieve miniaturized 3D microcapsules (sphere, cuboid, and rod shape). By this proposed method, rat liver cells (RLC-18) formed multi-cellular aggregates with high cell-density after cultivation for 2 weeks.
journal article
Elsevier
2016-06
application/pdf
Acta Biomaterialia
37
93
100
http://dx.doi.org/10.1016/j.actbio.2016.03.045
http://hdl.handle.net/2237/25108
1742-7061
https://nagoya.repo.nii.ac.jp/record/22928/files/Paper_for_nagoya.pdf
eng
https://doi.org/10.1016/j.actbio.2016.03.045
© 2016. This manuscript version is made available under the CC-BY-NC-ND 4.0 license http://creativecommons.org/licenses/by-nc-nd/4.0/