2024-03-29T13:11:40Z
https://nagoya.repo.nii.ac.jp/oai
oai:nagoya.repo.nii.ac.jp:00022965
2023-01-16T04:11:36Z
320:321:322
Constitutively expressed Siglec-9 inhibits LPS-induced CCR7, but enhances IL-4-induced CD200R expression in human macrophages
Higuchi, Hiroshi
67772
Shoji, Toru
67773
Iijima, Shinji
67774
Nishijima, Ken-ichi
67775
human
lectin
macrophages
sialic acid
Siglec
Siglecs recognize the sialic acid moiety and regulate various immune responses. In the present study, we compared the expression levels of Siglecs in human monocytes and macrophages using a quantitative real-time reverse transcription–polymerase chain reaction analysis. The differentiation of monocytes into macrophages by macrophage colony-stimulating factor or granulocyte macrophage colony-stimulating factor enhanced the expression of Siglec-7 and Siglec-9. The differentiated macrophages were stimulated by lipopolysaccharide (LPS) plus interferon (IFN)-γ or interleukin (IL)-4. The expression of Siglec-10 was enhanced by IL-4, whereas that of Siglec-7 was reduced by LPS plus IFN-γ. The expression of Siglec-9 was not affected by these stimuli. The knockdown of Siglec-9 enhanced the expression of CCR7 induced by the LPS or the LPS plus IFN-γ stimulation, and decreased the IL-4-induced expression of CD200R. These results suggest that Siglec-9 is one of the main Siglecs in human blood monocytes/macrophages and modulates innate immunity.
journal article
Taylor & Francis
2016-02-29
application/pdf
Bioscience, Biotechnology, and Biochemistry
6
80
1141
1148
http://doi.org/10.1080/09168451.2016.1146070
http://hdl.handle.net/2237/25146
0916-8451
https://nagoya.repo.nii.ac.jp/record/22965/files/Higuchi_et_al.pdf
eng
https://doi.org/10.1080/09168451.2016.1146070
(c)日本農芸化学会. This is an Accepted Manuscript of an article published by Taylor & Francis Group in Bioscience, Biotechnology, and Biochemistry on 29/02/2016, available online: http://www.tandfonline.com/10.1080/09168451.2016.1146070.