2024-03-28T23:46:51Z
https://nagoya.repo.nii.ac.jp/oai
oai:nagoya.repo.nii.ac.jp:00023208
2023-01-16T05:06:18Z
499:500:501
Induction of Epstein-Barr Virus Oncoprotein LMP1 by Transcription Factors AP-2 and Early B Cell Factor
Murata, Takayuki
68761
Noda, Chieko
68762
Narita, Yohei
68763
Watanabe, Takahiro
68764
Yoshida, Masahiro
68765
Ashio, Keiji
68766
Sato, Yoshitaka
68767
Goshima, Fumi
68768
Kanda, Teru
68769
Yoshiyama, Hironori
68770
Tsurumi, Tatsuya
68771
Kimura, Hiroshi
68772
Latent membrane protein 1 (LMP1) is a major oncogene essential for primary B cell transformation by Epstein-Barr virus (EBV). Previous studies suggested that some transcription factors, such as PU.1, RBP-Jκ, NF-κB, and STAT, are involved in this expression, but the underlying mechanism is unclear. Here, we identified binding sites for PAX5, AP-2, and EBF in the proximal LMP1 promoter (ED-L1p). We first confirmed the significance of PU.1 and POU domain transcription factor binding for activation of the promoter in latency III. We then focused on the transcription factors AP-2 and early B cell factor (EBF). Interestingly, among the three AP-2-binding sites in the LMP1 promoter, two motifs were also bound by EBF. Overexpression, knockdown, and mutagenesis in the context of the viral genome indicated that AP-2 plays an important role in LMP1 expression in latency II in epithelial cells. In latency III B cells, on the other hand, the B cell-specific transcription factor EBF binds to the ED-L1p and activates LMP1 transcription from the promoter.
journal article
American Society for Microbiology
2016-04
application/pdf
Journal of Virology
8
90
3873
3889
http://doi.org/10.1128/JVI.03227-15
http://hdl.handle.net/2237/25404
0022-538X
https://nagoya.repo.nii.ac.jp/record/23208/files/Murata_et_al_Jv2016.pdf
eng
https://doi.org/10.1128/JVI.03227-15