2024-03-29T06:00:19Z
https://nagoya.repo.nii.ac.jp/oai
oai:nagoya.repo.nii.ac.jp:00024631
2023-01-16T04:15:04Z
499:500:501
Exposure to diphtheria toxin during the juvenile period impairs both inner and outer hair cells in C57BL/6 mice
Konishi, Hiroyuki
72915
Ohgami, Nobutaka
72916
Matsushita, Aika
72917
Kondo, Yuki
72918
Aoyama, Yuki
72919
Kobayashi, Masaaki
72920
Nagai, Taku
72921
Ugawa, Shinya
72922
Yamada, Kiyofumi
72923
Kato, Masashi
72924
Kiyama, Hiroshi
72925
cochlea
degeneration
diphtheria toxin
hair cell
hearing loss
ototoxicity
Diphtheria toxin (DT) administration into transgenic mice that express the DT receptor (DTR) under control of specific promoters is often used for cell ablation studies in vivo. Because DTR is not expressed in mice, DT injection has been assumed to be nontoxic to cells in vivo. In this study, we demonstrated that DT application during the juvenile stage leads to hearing loss in wild-type mice. Auditory brainstem response measurement showed severe hearing loss in C57BL/6 mice administered DT during the juvenile period, and the hearing loss persisted into adulthood. However, ototoxicity did not occur when DT was applied on postnatal day 28 or later. Histological studies demonstrated that hearing loss was accompanied by significant degeneration of inner and outer hair cells (HCs), as well as spiral ganglion neurons. Scanning electron microscopy showed quick degeneration of inner HCs within 3 days and gradual degeneration of outer HCs within 1 week. These results demonstrated that DT has ototoxic action on C57BL/6 mice during the juvenile period, but not thereafter, and the hearing loss was due to degeneration of inner and outer HCs by unknown DT-related mechanisms.
journal article
Elsevier
2017-05-20
application/pdf
Neuroscience
351
15
23
https://doi.org/10.1016/j.neuroscience.2017.03.028
http://hdl.handle.net/2237/26850
0306-4522
https://nagoya.repo.nii.ac.jp/record/24631/files/Konishi_Neuroscience_2017.pdf
eng
https://doi.org/10.1016/j.neuroscience.2017.03.028
© 2017. This manuscript version is made available under the CC-BY-NC-ND 4.0 license http://creativecommons.org/licenses/by-nc-nd/4.0/