2024-03-29T07:19:47Z
https://nagoya.repo.nii.ac.jp/oai
oai:nagoya.repo.nii.ac.jp:00024856
2023-01-16T04:30:25Z
1179:2033:2034
Alpha-2 macroglobulin as a region-specific secretory protein in male reproductive tract, and its dynamics during sperm transit toward the female spermatheca in the blue crab
yaporn, Senarai
73932
Rapeepun, Vanichviriyakit
73933
Shinji, Miyata
73934
Chihiro, Sato
73935
Prapee, Sretarugsa
73936
Wattana, Weerachatyanukul
73937
Ken, Kitajima
73938
crab sperm
male reproductive tract
protease inhibitor
seminal fluid protein
sperm maturation
A 250-kDa protein was isolated from fluid in the middle spermatic duct (MSD) of the blue crab (Portunus pelagicus). N-terminal and partial amino acid sequences revealed that this MSD-specific protein is highly similar to the plasma-enriched protein Alpha-2 macroglobulin (α2M). The P. pelagicus ortholog (Ppα2M) is a large glycoprotein possessing mannose and N-acetylglucosamine residues. Ppa2m mRNA was detected in the spermatic duct, androgenic gland, and hematopoietic tissue, whereas the protein was primarily observed in the apical cytoplasm of MSD epithelium and in the matrix of the acrosome of MSD sperm; distally within spermatic duct, Ppα2M was lost from the sperm membrane but remained in the sperm acrosome. These results suggest that Ppα2M is expressed and glycosylated in the epithelium of spermatic ducts, secreted into MSD fluid, taken up by sperm in the MSD, and removed from the surface of sperm during its transit towards the female spermatheca. Given that Ppα2M also exhibits protease inhibitor activity, we hypothesize that acrosome localized Ppα2M may suppress premature acrosome reaction during post-testicular sperm maturation in this crab.
journal article
Wiley
2017-07
application/pdf
Molecular Reproduction and Development
7
84
585
595
http://doi.org/10.1002/mrd.22817
http://hdl.handle.net/2237/27076
1040-452X
https://nagoya.repo.nii.ac.jp/record/24856/files/TSenarai_FinalMRD2017.pdf
eng
https://doi.org/10.1002/mrd.22817
This is the peer reviewed version of the following article: [MOLECULAR REPRODUCTION AND DEVELOPMENT. v.84, n.7, 2017, p.585-595], which has been published in final form at [http://doi.org/10.1002/mrd.22817]. This article may be used for non-commercial purposes in accordance with Wiley Terms and Conditions for Self-Archiving.