2024-03-28T17:39:35Z
https://nagoya.repo.nii.ac.jp/oai
oai:nagoya.repo.nii.ac.jp:00028697
2023-01-16T04:21:41Z
643:666:667
Clavariopsins C–I, Antifungal Cyclic Depsipeptides from the Aquatic Hyphomycete Clavariopsis aquatica
Soe, Thin Wut
94195
Han, Chunguang
94196
Fudou, Ryosuke
94197
Kaida, Kenichi
94198
Sawaki, Yuki
94199
Tomura, Tomohiko
94200
Ojika, Makoto
94201
Seven new cyclic depsipeptides, clavariopsins C–I (3–9), together with two known congeners, clavariopsins A and B (1 and 2), were isolated from the aquatic hyphomycete Clavariopsis aquatica. Their planar structures, which consist of nine amino acids and one α-hydroxy acid, were elucidated by NMR spectroscopy and HRESIMS. The absolute configurations were established by the advanced Marfey’s method and chiral-phase HPLC analysis. Their antifungal and cytotoxic activities were evaluated against six plant pathogenic fungi (Botrytis cinerea, Magnaporthe oryzae, Colletotrichum orbiculare, Fusarium oxysporum, Alternaria alternata, and Aspergillus niger) and a cancer cell line (HeLa-S3), respectively. The majority of the compounds exhibited potent antifungal activity against the fungi tested (minimum inhibition dose = 0.01–10 μg/disk) and induced hyphal swelling in A. niger (minimum effective dose = 0.3–3 μg/disk), whereas the compounds exhibited no cytotoxicity toward the cancer cell line. The results suggest that the clavariopsins could be a promising class of antifungal agents.
ファイル公開:2020-07-26
journal article
ACS Publications
2019-07-26
application/pdf
Journal of Natural Products
7
82
1971
1978
0163-3864
https://nagoya.repo.nii.ac.jp/record/28697/files/JNP_revised manuscript.pdf
eng
https://doi.org/10.1021/acs.jnatprod.9b00366
“This document is the Accepted Manuscript version of a Published Work that appeared in final form in [Journal of Natural Products], copyright © American Chemical Society after peer review and technical editing by the publisher. To access the final edited and published work see [https://pubs.acs.org/articlesonrequest/AOR-tUsIHfzPbDUzbGeAcMhM].”