2024-03-29T14:40:26Z
https://nagoya.repo.nii.ac.jp/oai
oai:nagoya.repo.nii.ac.jp:00030516
2023-01-16T04:24:04Z
336:695:696
Formation of the High-Spin S2 State Related to the Extrinsic Proteins in the Oxygen Evolving Complex of Photosystem II
Taguchi, Shota
101584
Shen, Liangliang
101585
Han, Guangye
101586
Umena, Yasufumi
101587
Shen, Jian-Ren
101588
Noguchi, Takumi
101589
Mino, Hiroyuki
101590
The high-spin S2 state was investigated with photosystem II (PSII) from spinach, Thermosynechococcus vulcanus, and Cyanidioschyzon merolae. In extrinsic protein-depleted PSII, high-spin electron paramagnetic resonance (EPR) signals were not detected in either species, whereas all species showed g ∼ 5 signals in the presence of a high concentration of Ca^2+ instead of the multiline signal. In the intact and PsbP/Q-depleted PSII from spinach, the g = 4.1 EPR signal was detected. These results show that formation of the high-spin S2 state of the manganese cluster is regulated by the extrinsic proteins through a charge located near the Mn4 atom in the Mn4CaO5 cluster but is independent of the intrinsic proteins. The shift to the g ∼ 5 state is caused by tilting of the z-axis in the Mn4 coordinates through hydrogen bonds or external divalent cations. The structural modification may allow insertion of an oxygen atom during the S2-to-S3 transition.
Published online: 29 September 2020 ファイル公開:2021-09-29
journal article
ACS Publications
2020-10
application/pdf
application/pdf
The Journal of Physical Chemistry Letters
20
11
8908
8913
1948-7185
https://nagoya.repo.nii.ac.jp/record/30516/files/200926_manuscript_JPCL_pr.pdf
https://nagoya.repo.nii.ac.jp/record/30516/files/jz0c02411_si_001.pdf
eng
https://doi.org/10.1021/acs.jpclett.0c02411
“This document is the Accepted Manuscript version of a Published Work that appeared in final form in [The Journal of Physical Chemistry B], copyright © American Chemical Society after peer review and technical editing by the publisher. To access the final edited and published work see [https://pubs.acs.org/articlesonrequest/AOR-UCBYCVYETEJEWMH7QIBW].”