@article{oai:nagoya.repo.nii.ac.jp:00011941,
 author = {Cao, Yu-An and Blair, James B. and Ostrander, Gary K.},
 journal = {The Fish Biology Journal Medaka},
 month = {},
 note = {We report on the establishment of primary liver cell cultures from medaka, Oryzias latipes. We utilized serum-free media, positively-charged Primaria dishes, and collagenase for disassociation of medaka liver cells to establish initial culture conditions. Nearly homogenous (about 96%) primary hepatocyte cultures were initiated and remained viable and proliferative for 30 days. Cultured cells exhibited either a cuboidal/epithelial-like phenotype or spindle-shaped phenotype. Both cell types appeared to have been derived from liver epithelia, as verified by western blotting of cytokeratins and transmission electron microscopy. The predominate cell type was the spindle cell which proliferated to confluence in primary culture. The initial behavior, proliferation characteristics, and media requirements for attachment and spreading of these cells are described. Primary cultures of medaka liver cells have the potential to serve as a useful model for a variety of investigations including, but not limited to, liver cell development, function, and xenobiotic metabolism in an established fish model., Regular Paper},
 pages = {47--56},
 title = {The Initial Report of the Establishment of Primary Liver Cell Cultures from Medaka (Oryzias latipes)},
 volume = {8},
 year = {1996}
}