{"_buckets": {"deposit": "1d82d818-400f-4b04-9386-63a1b11e4877"}, "_deposit": {"id": "11949", "owners": [], "pid": {"revision_id": 0, "type": "depid", "value": "11949"}, "status": "published"}, "_oai": {"id": "oai:nagoya.repo.nii.ac.jp:00011949", "sets": ["1257"]}, "author_link": ["37864"], "item_1615768549627": {"attribute_name": "出版タイプ", "attribute_value_mlt": [{"subitem_version_resource": "http://purl.org/coar/version/c_970fb48d4fbd8a85", "subitem_version_type": "VoR"}]}, "item_9_biblio_info_6": {"attribute_name": "書誌情報", "attribute_value_mlt": [{"bibliographicIssueDates": {"bibliographicIssueDate": "1997", "bibliographicIssueDateType": "Issued"}, "bibliographicPageEnd": "50", "bibliographicPageStart": "49", "bibliographicVolumeNumber": "9", "bibliographic_titles": [{"bibliographic_title": "The Fish Biology Journal Medaka", "bibliographic_titleLang": "en"}]}]}, "item_9_description_4": {"attribute_name": "抄録", "attribute_value_mlt": [{"subitem_description": "Medaka embryos are covered by a transparent but hard chorion. The chorion makes embryonic manipulation in this species difficult. The medaka hatching emzyme is secreted from the hatching enzyme gland at hatching. Dechorionation of embryos using the enzyme facilitates embryonic manipulation such as transplantation of embryonic cells between embryos, and enables removal of embryos from the chorion for detailed observation or direct treatment. Here, I introduce an easy method for preparation of the enzyme solution from medaka embryos. This method was developed by the staff and students of our laboratory by modification of the previous method reported by Yasumasu, et al. (1989). According to this method, 15 ml of the enzyme solution, which dissolves the chorion within 50 to 60 min, can be prepared using about 5,000 embryos.", "subitem_description_language": "en", "subitem_description_type": "Abstract"}]}, "item_9_description_5": {"attribute_name": "内容記述", "attribute_value_mlt": [{"subitem_description": "Technical Report", "subitem_description_language": "en", "subitem_description_type": "Other"}]}, "item_9_identifier_60": {"attribute_name": "URI", "attribute_value_mlt": [{"subitem_identifier_type": "HDL", "subitem_identifier_uri": "http://hdl.handle.net/2237/13823"}]}, "item_9_identifier_registration": {"attribute_name": "ID登録", "attribute_value_mlt": [{"subitem_identifier_reg_text": "10.18999/fisbjm.9.49", "subitem_identifier_reg_type": "JaLC"}]}, "item_9_publisher_32": {"attribute_name": "出版者", "attribute_value_mlt": [{"subitem_publisher": "Laboratory of Freshwater Fish Stocks Bioscience Center Nagoya University", "subitem_publisher_language": "en"}]}, "item_9_relation_43": {"attribute_name": "関連情報", "attribute_value_mlt": [{"subitem_relation_type": "isVersionOf", "subitem_relation_type_id": {"subitem_relation_type_id_text": "http://ci.nii.ac.jp/naid/110007349034/", "subitem_relation_type_select": "URI"}}]}, "item_9_rights_12": {"attribute_name": "権利", "attribute_value_mlt": [{"subitem_rights": "Laboratory of Freshwater Fish Stocks Bioscience Center Nagoya University", "subitem_rights_language": "en"}, {"subitem_rights": "本文データは学協会の許諾に基づきCiNiiから複製したものである", "subitem_rights_language": "ja"}]}, "item_9_select_15": {"attribute_name": "著者版フラグ", "attribute_value_mlt": [{"subitem_select_item": "publisher"}]}, "item_9_text_14": {"attribute_name": "フォーマット", "attribute_value_mlt": [{"subitem_text_value": "application/pdf"}]}, "item_access_right": {"attribute_name": "アクセス権", "attribute_value_mlt": [{"subitem_access_right": "open access", "subitem_access_right_uri": "http://purl.org/coar/access_right/c_abf2"}]}, "item_creator": {"attribute_name": "著者", "attribute_type": "creator", "attribute_value_mlt": [{"creatorNames": [{"creatorName": "Wakamatsu, Yuko", "creatorNameLang": "en"}], "nameIdentifiers": [{"nameIdentifier": "37864", "nameIdentifierScheme": "WEKO"}]}]}, "item_files": {"attribute_name": "ファイル情報", "attribute_type": "file", "attribute_value_mlt": [{"accessrole": "open_date", "date": [{"dateType": "Available", "dateValue": "2018-02-20"}], "displaytype": "detail", "download_preview_message": "", "file_order": 0, "filename": "KJ00005673928.pdf", "filesize": [{"value": "147.2 kB"}], "format": "application/pdf", "future_date_message": "", "is_thumbnail": false, "licensetype": "license_note", "mimetype": "application/pdf", "size": 147200.0, "url": {"label": "KJ00005673928.pdf", "objectType": "fulltext", "url": "https://nagoya.repo.nii.ac.jp/record/11949/files/KJ00005673928.pdf"}, "version_id": "e9553826-b1df-4207-bd2e-aaa9ab64262d"}]}, "item_language": {"attribute_name": "言語", "attribute_value_mlt": [{"subitem_language": "eng"}]}, "item_resource_type": {"attribute_name": "資源タイプ", "attribute_value_mlt": [{"resourcetype": "departmental bulletin paper", "resourceuri": "http://purl.org/coar/resource_type/c_6501"}]}, "item_title": "Preparation of Medaka Hatching Enzyme", "item_titles": {"attribute_name": "タイトル", "attribute_value_mlt": [{"subitem_title": "Preparation of Medaka Hatching Enzyme", "subitem_title_language": "en"}]}, "item_type_id": "9", "owner": "1", "path": ["1257"], "permalink_uri": "https://doi.org/10.18999/fisbjm.9.49", "pubdate": {"attribute_name": "PubDate", "attribute_value": "2010-06-21"}, "publish_date": "2010-06-21", "publish_status": "0", "recid": "11949", "relation": {}, "relation_version_is_last": true, "title": ["Preparation of Medaka Hatching Enzyme"], "weko_shared_id": -1}