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  1. H180 生物機能開発利用研究センター
  2. H180b 紀要
  3. The fish biology journal Medaka
  4. 9

Preparation of Medaka Hatching Enzyme

https://doi.org/10.18999/fisbjm.9.49
https://doi.org/10.18999/fisbjm.9.49
7444f438-b747-46cf-970f-d474dbe8befb
名前 / ファイル ライセンス アクション
KJ00005673928.pdf KJ00005673928.pdf (147.2 kB)
Item type 紀要論文 / Departmental Bulletin Paper(1)
公開日 2010-06-21
タイトル
タイトル Preparation of Medaka Hatching Enzyme
言語 en
著者 Wakamatsu, Yuko

× Wakamatsu, Yuko

WEKO 37864

en Wakamatsu, Yuko

Search repository
アクセス権
アクセス権 open access
アクセス権URI http://purl.org/coar/access_right/c_abf2
権利
言語 en
権利情報 Laboratory of Freshwater Fish Stocks Bioscience Center Nagoya University
権利
言語 ja
権利情報 本文データは学協会の許諾に基づきCiNiiから複製したものである
抄録
内容記述 Medaka embryos are covered by a transparent but hard chorion. The chorion makes embryonic manipulation in this species difficult. The medaka hatching emzyme is secreted from the hatching enzyme gland at hatching. Dechorionation of embryos using the enzyme facilitates embryonic manipulation such as transplantation of embryonic cells between embryos, and enables removal of embryos from the chorion for detailed observation or direct treatment. Here, I introduce an easy method for preparation of the enzyme solution from medaka embryos. This method was developed by the staff and students of our laboratory by modification of the previous method reported by Yasumasu, et al. (1989). According to this method, 15 ml of the enzyme solution, which dissolves the chorion within 50 to 60 min, can be prepared using about 5,000 embryos.
言語 en
内容記述タイプ Abstract
内容記述
内容記述 Technical Report
言語 en
内容記述タイプ Other
出版者
言語 en
出版者 Laboratory of Freshwater Fish Stocks Bioscience Center Nagoya University
言語
言語 eng
資源タイプ
資源 http://purl.org/coar/resource_type/c_6501
タイプ departmental bulletin paper
出版タイプ
出版タイプ VoR
出版タイプResource http://purl.org/coar/version/c_970fb48d4fbd8a85
ID登録
ID登録 10.18999/fisbjm.9.49
ID登録タイプ JaLC
関連情報
関連タイプ isVersionOf
識別子タイプ URI
関連識別子 http://ci.nii.ac.jp/naid/110007349034/
書誌情報 en : The Fish Biology Journal Medaka

巻 9, p. 49-50, 発行日 1997
フォーマット
application/pdf
著者版フラグ
値 publisher
URI
識別子 http://hdl.handle.net/2237/13823
識別子タイプ HDL
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