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Preparation of Medaka Hatching Enzyme
https://doi.org/10.18999/fisbjm.9.49
https://doi.org/10.18999/fisbjm.9.497444f438-b747-46cf-970f-d474dbe8befb
名前 / ファイル | ライセンス | アクション |
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Item type | 紀要論文 / Departmental Bulletin Paper(1) | |||||
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公開日 | 2010-06-21 | |||||
タイトル | ||||||
タイトル | Preparation of Medaka Hatching Enzyme | |||||
言語 | en | |||||
著者 |
Wakamatsu, Yuko
× Wakamatsu, Yuko |
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アクセス権 | ||||||
アクセス権 | open access | |||||
アクセス権URI | http://purl.org/coar/access_right/c_abf2 | |||||
権利 | ||||||
言語 | en | |||||
権利情報 | Laboratory of Freshwater Fish Stocks Bioscience Center Nagoya University | |||||
権利 | ||||||
言語 | ja | |||||
権利情報 | 本文データは学協会の許諾に基づきCiNiiから複製したものである | |||||
抄録 | ||||||
内容記述 | Medaka embryos are covered by a transparent but hard chorion. The chorion makes embryonic manipulation in this species difficult. The medaka hatching emzyme is secreted from the hatching enzyme gland at hatching. Dechorionation of embryos using the enzyme facilitates embryonic manipulation such as transplantation of embryonic cells between embryos, and enables removal of embryos from the chorion for detailed observation or direct treatment. Here, I introduce an easy method for preparation of the enzyme solution from medaka embryos. This method was developed by the staff and students of our laboratory by modification of the previous method reported by Yasumasu, et al. (1989). According to this method, 15 ml of the enzyme solution, which dissolves the chorion within 50 to 60 min, can be prepared using about 5,000 embryos. | |||||
言語 | en | |||||
内容記述タイプ | Abstract | |||||
内容記述 | ||||||
内容記述 | Technical Report | |||||
言語 | en | |||||
内容記述タイプ | Other | |||||
出版者 | ||||||
言語 | en | |||||
出版者 | Laboratory of Freshwater Fish Stocks Bioscience Center Nagoya University | |||||
言語 | ||||||
言語 | eng | |||||
資源タイプ | ||||||
資源 | http://purl.org/coar/resource_type/c_6501 | |||||
タイプ | departmental bulletin paper | |||||
出版タイプ | ||||||
出版タイプ | VoR | |||||
出版タイプResource | http://purl.org/coar/version/c_970fb48d4fbd8a85 | |||||
ID登録 | ||||||
ID登録 | 10.18999/fisbjm.9.49 | |||||
ID登録タイプ | JaLC | |||||
関連情報 | ||||||
関連タイプ | isVersionOf | |||||
識別子タイプ | URI | |||||
関連識別子 | http://ci.nii.ac.jp/naid/110007349034/ | |||||
書誌情報 |
en : The Fish Biology Journal Medaka 巻 9, p. 49-50, 発行日 1997 |
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フォーマット | ||||||
application/pdf | ||||||
著者版フラグ | ||||||
値 | publisher | |||||
URI | ||||||
識別子 | http://hdl.handle.net/2237/13823 | |||||
識別子タイプ | HDL |