@article{oai:nagoya.repo.nii.ac.jp:00011949,
 author = {Wakamatsu, Yuko},
 journal = {The Fish Biology Journal Medaka},
 month = {},
 note = {Medaka embryos are covered by a transparent but hard chorion. The chorion makes embryonic manipulation in this species difficult. The medaka hatching emzyme is secreted from the hatching enzyme gland at hatching. Dechorionation of embryos using the enzyme facilitates embryonic manipulation such as transplantation of embryonic cells between embryos, and enables removal of embryos from the chorion for detailed observation or direct treatment. Here, I introduce an easy method for preparation of the enzyme solution from medaka embryos. This method was developed by the staff and students of our laboratory by modification of the previous method reported by Yasumasu, et al. (1989). According to this method, 15 ml of the enzyme solution, which dissolves the chorion within 50 to 60 min, can be prepared using about 5,000 embryos., Technical Report},
 pages = {49--50},
 title = {Preparation of Medaka Hatching Enzyme},
 volume = {9},
 year = {1997}
}