{"created":"2021-03-01T06:19:30.940753+00:00","id":12550,"links":{},"metadata":{"_buckets":{"deposit":"ca88fa7e-9ce9-428c-ab3c-115dc9cab78f"},"_deposit":{"id":"12550","owners":[],"pid":{"revision_id":0,"type":"depid","value":"12550"},"status":"published"},"_oai":{"id":"oai:nagoya.repo.nii.ac.jp:00012550","sets":["499:960:961"]},"author_link":["39468","39469"],"item_12_alternative_title_19":{"attribute_name":"その他のタイトル","attribute_value_mlt":[{"subitem_alternative_title":"ヒト神経芽細胞腫細胞株SH-SY5Y細胞のATRA誘導性転写因子COUP-TFIによるセラミドキナーゼ遺伝子の発現抑制","subitem_alternative_title_language":"ja"}]},"item_12_biblio_info_6":{"attribute_name":"書誌情報","attribute_value_mlt":[{"bibliographicIssueDates":{"bibliographicIssueDate":"2010-03-25","bibliographicIssueDateType":"Issued"}}]},"item_12_date_granted_64":{"attribute_name":"学位授与年月日","attribute_value_mlt":[{"subitem_dategranted":"2010-03-25"}]},"item_12_degree_grantor_62":{"attribute_name":"学位授与機関","attribute_value_mlt":[{"subitem_degreegrantor":[{"subitem_degreegrantor_language":"ja","subitem_degreegrantor_name":"名古屋大学"},{"subitem_degreegrantor_language":"en","subitem_degreegrantor_name":"NAGOYA University"}],"subitem_degreegrantor_identifier":[{"subitem_degreegrantor_identifier_name":"13901","subitem_degreegrantor_identifier_scheme":"kakenhi"}]}]},"item_12_degree_name_61":{"attribute_name":"学位名","attribute_value_mlt":[{"subitem_degreename":"博士(医療技術学)","subitem_degreename_language":"ja"}]},"item_12_description_4":{"attribute_name":"抄録","attribute_value_mlt":[{"subitem_description":"Ceramide is the central lipid in the sphingolipid metabolism. Ceramide kinase (CERK) and its product, ceramide 1-phosphate (C1P), have been implicated in various cellular functions. However, the regulatory mechanism of CERK gene expression remains to be determined. Here, we examined CERK mRNA level during all-trans retinoic acid (ATRA)-induced differentiation of a human neuroblastoma cell line, SH-SY5Y. ATRA reduced CERK mRNA and protein levels. Overexpression and siRNA of CERK revealed that CERK is inhibitory against ATRA-induced neuronal differentiation and cell growth arrest. ATRA inhibited the transcriptional activity of 5’-promoter of CERK. Truncation and mutation study suggests that ATRA-responsible region was mainly located in the tandem retinoic acid responsive elements (RARE) between -40 bp and the first exon. The electrophoresis mobility shift assay revealed that ATRA produced two retarded bands, which were erased by antibody against COUP-TFI, RARα and RXRα, respectively. DNA pull-down assay confirmed increased binding of these transcription factors to RARE. Transient expression of RAR, RXR and COUP-TFI and siRNA transfection of these genes revealed that COUP-TFI inhibited CERK mRNA. Furthermore, chromatin immunoprecipitation assay showed the recruitment of co-repressors as well as three transcription factors. These results suggest that COUP-TFI was the ATRA-responsive suppressive transcription factor of CERK gene transcription.","subitem_description_language":"en","subitem_description_type":"Abstract"}]},"item_12_description_5":{"attribute_name":"内容記述","attribute_value_mlt":[{"subitem_description":"名古屋大学博士学位論文 学位の種類:博士（医療技術学） (課程) 学位授与年月日　平成22年3月25日","subitem_description_language":"ja","subitem_description_type":"Other"}]},"item_12_dissertation_number_65":{"attribute_name":"学位授与番号","attribute_value_mlt":[{"subitem_dissertationnumber":"甲第8661号"}]},"item_12_identifier_60":{"attribute_name":"URI","attribute_value_mlt":[{"subitem_identifier_type":"HDL","subitem_identifier_uri":"http://hdl.handle.net/2237/14433"}]},"item_12_select_15":{"attribute_name":"著者版フラグ","attribute_value_mlt":[{"subitem_select_item":"publisher"}]},"item_12_text_63":{"attribute_name":"学位授与年度","attribute_value_mlt":[{"subitem_text_value":"2009"}]},"item_access_right":{"attribute_name":"アクセス権","attribute_value_mlt":[{"subitem_access_right":"open access","subitem_access_right_uri":"http://purl.org/coar/access_right/c_abf2"}]},"item_creator":{"attribute_name":"著者","attribute_type":"creator","attribute_value_mlt":[{"creatorNames":[{"creatorName":"村上, 真史","creatorNameLang":"ja"}],"nameIdentifiers":[{"nameIdentifier":"39468","nameIdentifierScheme":"WEKO"}]},{"creatorNames":[{"creatorName":"MURAKAMI, Masashi","creatorNameLang":"en"}],"nameIdentifiers":[{"nameIdentifier":"39469","nameIdentifierScheme":"WEKO"}]}]},"item_files":{"attribute_name":"ファイル情報","attribute_type":"file","attribute_value_mlt":[{"accessrole":"open_date","date":[{"dateType":"Available","dateValue":"2018-02-20"}],"displaytype":"detail","filename":"k8661.pdf","filesize":[{"value":"810.3 kB"}],"format":"application/pdf","licensetype":"license_note","mimetype":"application/pdf","url":{"label":"k8661.pdf","objectType":"fulltext","url":"https://nagoya.repo.nii.ac.jp/record/12550/files/k8661.pdf"},"version_id":"73285fb8-3f48-45fa-8772-c15da8a8b776"}]},"item_keyword":{"attribute_name":"キーワード","attribute_value_mlt":[{"subitem_subject":"All-trans retinoic acid","subitem_subject_scheme":"Other"},{"subitem_subject":"neuronal differentiation","subitem_subject_scheme":"Other"},{"subitem_subject":"SH-SY5Y cell","subitem_subject_scheme":"Other"},{"subitem_subject":"ceramide kinase","subitem_subject_scheme":"Other"},{"subitem_subject":"promoter analysis","subitem_subject_scheme":"Other"},{"subitem_subject":"COUP-TFI","subitem_subject_scheme":"Other"}]},"item_language":{"attribute_name":"言語","attribute_value_mlt":[{"subitem_language":"eng"}]},"item_resource_type":{"attribute_name":"資源タイプ","attribute_value_mlt":[{"resourcetype":"doctoral thesis","resourceuri":"http://purl.org/coar/resource_type/c_db06"}]},"item_title":"ATRA inhibits ceramide kinase transcription through an ATRA-related transcription factor, COUP-TFI, in a human neuroblastoma cell line, SH-SY5Y","item_titles":{"attribute_name":"タイトル","attribute_value_mlt":[{"subitem_title":"ATRA inhibits ceramide kinase transcription through an ATRA-related transcription factor, COUP-TFI, in a human neuroblastoma cell line, SH-SY5Y","subitem_title_language":"en"}]},"item_type_id":"12","owner":"1","path":["961"],"pubdate":{"attribute_name":"PubDate","attribute_value":"2011-01-07"},"publish_date":"2011-01-07","publish_status":"0","recid":"12550","relation_version_is_last":true,"title":["ATRA inhibits ceramide kinase transcription through an ATRA-related transcription factor, COUP-TFI, in a human neuroblastoma cell line, SH-SY5Y"],"weko_creator_id":"1","weko_shared_id":-1},"updated":"2023-01-16T03:59:15.911402+00:00"}