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  1. B200 工学部/工学研究科
  2. B200a 雑誌掲載論文
  3. 学術雑誌

Optical pH Regulation Using Photochromic Material for Selective Cell Injection of Nanosensors

http://hdl.handle.net/2237/14476
http://hdl.handle.net/2237/14476
f86bf067-2b1a-444f-8e20-3d449a224ad7
名前 / ファイル ライセンス アクション
1051.pdf 1051.pdf (1.3 MB)
Item type 学術雑誌論文 / Journal Article(1)
公開日 2011-02-15
タイトル
タイトル Optical pH Regulation Using Photochromic Material for Selective Cell Injection of Nanosensors
言語 en
著者 Maruyama, Hisataka

× Maruyama, Hisataka

WEKO 39615

en Maruyama, Hisataka

Search repository
Inoue, Naoya

× Inoue, Naoya

WEKO 39616

en Inoue, Naoya

Search repository
Arai, Fumihito

× Arai, Fumihito

WEKO 39617

en Arai, Fumihito

Search repository
アクセス権
アクセス権 open access
アクセス権URI http://purl.org/coar/access_right/c_abf2
権利
言語 en
権利情報 © 2011 IEEE. Personal use of this material is permitted. Permission from IEEE must be obtained for all other uses, in any current or future media, including reprinting/republishing this material for advertising or promotional purposes, creating new collective works, for resale or redistribution to servers or lists, or reuse of any copyrighted component of this work in other works.
抄録
内容記述 We developed Optical pH regulation using gel tool impregnated with photochromic material for selective cell injection of nanosensor for intracellular measurement. The nanosensor was included in the fusogenic liposome. Membrane fusion of the liposome to the cell membrane was used for invasive cell injection of the nanosensor. The liposome fuses to the cell in weak acidic condition. Local pH regulation inside the liposome was developed using photochromic material included in the nanotool. The nanosensor was modified by fluorescent dye for environment measurement. The nanosensor was also modified by Leuco crystal violet (LCV). LCV is photochromic material. LCV emits the proton by ultraviolet (UV) illumination. The emitted proton decreases the pH value in the liposome. This pH regulation is reversible by UV/VIS illumination. The nanosensor included in the liposome was manipulated by optical tweezers. After contact of the liposome to the cell membrane, the liposome was adhered to the cell membrane by UV induced membrane fusion. The nanosensor was injected into the cell. Injected nanosensor was manipulated by optical tweezers. Intracellular temperature was detected by measuring the fluorescence intensity of the nanosensor. We demonstrated optical pH regulation, UV induced cell injection of the nanosensor, and manipulation of the nanosensor in the cell.
言語 en
内容記述タイプ Abstract
出版者
言語 en
出版者 IEEE
言語
言語 eng
資源タイプ
資源タイプresource http://purl.org/coar/resource_type/c_6501
タイプ journal article
出版タイプ
出版タイプ AM
出版タイプResource http://purl.org/coar/version/c_ab4af688f83e57aa
DOI
関連タイプ isVersionOf
識別子タイプ DOI
関連識別子 https://doi.org/10.1109/NMDC.2010.5652526
ISBN
関連タイプ isPartOf
識別子タイプ ISBN
関連識別子 978-1-4244-8896-4
書誌情報 en : IEEE Nanotechnology Materials and Devices Conference (NMDC)

p. 57-60, 発行日 2010
著者版フラグ
値 author
URI
識別子 http://hdl.handle.net/2237/14476
識別子タイプ HDL
URI
識別子 http://dx.doi.org/10.1109/NMDC.2010.5652526
識別子タイプ DOI
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