@article{oai:nagoya.repo.nii.ac.jp:00013020,
 author = {KAMEGAYA, TAICHI and KURODA, KENJI and HAYAKAWA, YOSHIHIRO},
 issue = {1-2},
 journal = {Nagoya Journal of Medical Science},
 month = {Feb},
 note = {Here we show that bis(3’–5’) cyclic diadenylic acid (c-di-AMP) and a diadenylate cyclase (DAC) domain protein involved in the biosynthesis of c-di-AMP were identified in Streptococcus pyogenes. The matrix-assisted laser desorption ionization (MALDI)-time of flight (TOF) mass spectrum of the cell extract of S. pyogenes, which showed a fragment pattern very similar to that of the authentic sample of c-di-AMP, revealed that S. pyogenes produces c-di-AMP in the cell. Subsequently, we confirmed by an in vitro experiment that the production of c-di-AMP in the cell is due to the action of Spy1036 gene encoding a DAC domain protein named spyDAC, which is a new protein different from a well-known diadenylate cyclase. Moreover, the experiment gave a product with a molecular weight of 657.021, which is consistent with the molecular weight of c-di-AMP. Furthermore, the mass spectral fragment pattern of the product obtained by the in vitro biosynthesis is quite similar to that of the product produced by the above in vivo experiment. This in vitro production of c-di-AMP indicated that spyDAC in S. pyogenes actually catalyzes the in vivo biosynthesis of c-di-AMP from ATP., 名古屋大学博士学位論文 学位の種類 : 博士(医学)(課程) 学位授与年月日:平成23年3月25日 亀ヶ谷太一氏の博士論文として提出された},
 pages = {49--57},
 title = {IDENTIFICATION OF A STREPTOCOCCUS PYOGENES SF370 GENE INVOLVED IN PRODUCTION OF C-DI-AMP},
 volume = {73},
 year = {2011}
}